Hasan Hüseyin Hadimli

A comparative study on detection of Ornithobacterium rhinotracheale antibodies in meat-type turkeys by dot immunobinding assay, rapid agglutination test and serum agglutination test

The objectives of the present study were to develop a dot-immunobinding assay (DIA) and a serum agglutination test (SAT) for detection of Ornithobacterium rhinotracheale , to compare the rapid agglutination test (RAT) and the SAT, and to make a serosurvey of O. rhinotracheale exposure on turkey farms in Turkey. Antiserum against O. rhinotracheale bacterin was prepared in rabbits and 72 serum samples were collected from turkeys with respiratory signs on four farms. Comparison of the tests showed that 55.5, 48.6 and 40.3% of serum samples were positive by RAT, SAT and DIA, respectively. The sensitivity of the DIA appeared to be lower than that of the agglutination tests but the specificity is not known.

Rapid diagnosis of ovine Brucella, Campylobacter and Salmonella infections from fetal stomach contents by coagglutination test

Abstract A coagglutination test (CT) was developed to detect Brucella , Campylobacter and Salmonella antigens in stomach contents of 58 aborted sheep fetuses and compared with conventional culture techniques. Brucella melitensis , Campylobacter fetus and Salmonella abortus ovis were isolated from 31 (53.4%), 13 (22.4%) and 4 (6.9%) fetuses, respectively. Thirty-five of the fetal stomach contents were positive with the CT test, including 19 (61.3%) with Brucella , 12 (92.3%) with Campylobacter and 4 (100%) with Salmonella CT tests, respectively. Seven samples were negative with all three CT reagents. Cross-reactions were seen with C ampylobacter CT (11 samples) and S almonella CT (six samples). The sensitivity and specificity of the CT tests were 61 and 80% for Brucella , 92 and 80% for Campylobacter , and 100 and 90% for Salmonella , respectively. The CT was found to be technically simple and rapid in diagnosing Salmonella , Campylobacter or Brucella infections by comparison with conventional culture methods.

Detection and molecular typing of Clostridium perfringens isolates from beef, chicken and turkey meats

Here we describe a study investigating the presence of Clostridium perfringens strains in meat samples and the toxin genes in the isolates by PCR. This study, for the first time, demonstrated the presence of C. perfringens type E in turkey meats, while C. perfringens type C strains isolated from chicken meats.

Field evaluation of a PCR for the diagnosis of chlamydial abortion in sheep

A total of 94 vaginal swab samples and 195 serum samples collected from aborted ewes in 15 flocks were examined by pcr and a complement fixation test, respectively. In addition, 172 samples of stomach contents from fetuses from different flocks submitted for the diagnosis of abortion during the four lambing periods between 2000 and 2004 were tested by pcr. Chlamydial dna was detected in seven vaginal swabs obtained from five of the 15 flocks and in six samples of fetal stomach contents. The results of pcr and flock serology for Chlamydia were positive in five of the 15 flocks and negative in eight.

Antimicrobial effect of ozonated water, sodium hypochlorite and chlorhexidine gluconate in primary molar root canals

Objective: The aim was to determine the antimicrobial effect of ozonated water, ozonated water with ultrasonication, sodium hypochloride and chlorhexidine (CHX) in human primary root canals contaminated by Enterococcus faecalis (E. faecalis). Materials and Methods: Fifty-eight extracted human primary molar teeth were used. Crowns were cut off using a diamond saw under water-cooling. One hundred roots were obtained and mechanically prepared. The roots were then sterilized by autoclaving in water for 15 min at 121°C. All samples were contaminated with E. faecalis for 24 h and the root canals were randomly divided into five groups (n = 20). Group I: 25 mg/L of Ozonated water (O 3 aq), Group II: 25 mg/L of O 3 aq with ultrasonication, Group III: 2.5% Sodium hypochloride (NaOCl), Group IV: 2% CHX and Group V: Positive control. The canal of each specimen was irrigated for 4 min and positive control was untreated. All root canals were agitated with sterile saline solution. The saline solution was collected from canals with sterile paper points. For each specimen, the paper points were transposed to eppendorf vials containing 2 ml of brain heart infusion. According to bacterial proliferation, the mean values of optical density were achieved by ELέSA (Biotek EL ×800, Absorbance Microplate Reader, ABD) and the data were analyzed. Results: NaOCI, CHX and two types of O 3 aq were found statistically different than positive control group. NaOCI irrigation was found significantly most effective. Conclusions: NaOCl, CHX and O 3 aq applications provide antibacterial effect in vitro conditions in primary root canals.