Hasan Khatib

The distribution of the vWF alleles and genotypes in the Palestinian population.

Short tandem repeat (STR) loci amplified by PCR are known as a useful tool for individual identification and paternity testing. Direct PCR amplification from small amounts of whole blood is a rapid and convenient method for population screening for STR and VNTR markers. The allele frequencies of the vWF locus were determined for 127 unrelated Palestinians. Co-dominant segregation was observed in 20 mother/child pairs. Nine alleles were observed, with frequencies ranging from 0.004 to 0.327. Heterozygosity was 79%, and discrimination power was 0.927.

Allele frequencies of seven short tandem repeat loci in the Ashkenazi Jewish population

Allele frequencies of seven short tandem repeats (STRs) (THO1, CSF1PO, CD4, LPL, PLA2A, F13A01 and ARA) were obtained from a sample of unrelated Ashkenazi Jews living in Israel.

Tetranucleotide repeat polymorphism at the CCSP gene.

Technical comments: Blood samples were obtained in EDTA vacutainer from 95 unrelated Palestinian Arabs living in Jerusalem. Fifty p1 of whole blood were added to 500 p1 of 50 mM NaOH and boiled for 15 min. Then 100 pl of Tris-HCI 1 M (pH 8.0) were added to neutralize the solution. The samples were vortexed for 10 s, centrifuged for 2 min. and stored at 4°C till use. One p1 of the neutralized solution was amplified in each polymerase chain reaction (PCR) reaction as described (2) with the exception that the volume reaction was 13 pl. Table 1. Allele frequency of CCSP locus in a sample of 180 chromosomes of the Palestinian Arab population