Hatice Öge

Quantitative comparison of various methods for detecting eggs of Toxocara canis in samples of sand

Six techniques for recovering unembryonated Toxocara canis eggs from sand samples were tested for efficiency and suitability for routine use. The tests were done under standardised conditions on 50g of sand samples contaminated experimentally with 10, 100 and 500 eggs of T. canis. Best result was achieved by the method of Dunsmore et al. [Dunsmore, J.D., Thompson, R.C.A., Bates, I.A., 1984. Vet. Parasitol. 16, 303-311]. The results were expressed as the number of T. canis eggs recorded and percentage rates of recovery in sand samples.

The effect of doramectin, moxidectin and netobimin against natural infections of Syphacia muris in rats.

In this study, the effect of doramectin, moxidectin and netobimin was investigated in naturally infected Syphacia muris in rats. The natural infection was determined by the use of cellophane tape method on the perianal region and by the technique of centrifugal flotation of feces. The infected rats were divided into three treated and one control group (N = 10). Doramectin and moxidectin at the dose of 0.2 mg/kg per day and netobimin at the dose of 7.5 mg/kg per day were given in the diet for 4 days. Cellophane tape preparations were performed in all groups on 4th and 7th day after the last treatment. The rats of treated groups were necropsied on 7th day after the last treatment together with that of control group. While doramectin and netobimin were highly effective against S. muris, moxidectin was not found to be effective for eradication of S. muris.

Setaria equina infection of turkish equines: estimates of prevalence based on necropsy and the detection of microfilaraemia

Abstract Necropsies on 43 horses, 35 donkeys and two mules slaughtered in Ankara, Turkey, revealed that 12 (15%) of the equines harboured adult Setaria equina. When blood samples were checked for microfilariae, using Knotts method and a combination of membrane filtration followed by histochemical staining for acid phosphatase (AP), only three (4%) of the animals were found to be microfilaraemic. When stained for AP, the S. equina microfilariae exhibited diffuse red staining over the entire body, including the sheath, with brighter staining around the anal and excretory pores. Application of Knotts method revealed only two of the three microfilaraemias detected using the combination of filtration and AP staining. The combination may represent a useful technique for the detection of equine microfilaraemia.