Hattler Bg

Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation: II. Cellular

Mixed lymphocyte culture (MLC) reactivity has been followed in dogs before, during, and after rejection of heterotopically transplanted kidney allografts. During the time of active rejection, there was a loss of cellular reactivity in recipient (R) peripheral blood which could be ascribed to entrapment of immunoreactive cells in the rejecting kidneys. In this period, lymphocytes removed from blood draining the allograft had diminished MLC reactivity when compared to samples obtained from normal venous and arterial blood. Lymphocytes recovered from the rejecting organ demonstrated an accelerated MLC responsiveness to stimulating donor (D) lymphocytes at a time when lymphocytes from peripheral blood had diminished reactivity. Similar reactivity with kidney lymphocytes was also seen using stimulating lymphocytes of indifferent (I) dogs. Following rejection, when MLC responsiveness returned, a state of increased immunity could be demonstrated by an acceleration in the onset of reactivity after incubation in culture for shorter time periods.

Graft donor selection based upon single locus (haplotype) analysis within families.

Families can be genotyped with respect to their inheritance of the units of inheritance of the HL-A system (haplotypes). The conclusion that skin exchanged between siblings who are genotypically identical with respect to HL-A is accepted for prolonged periods has been confirmed. The antigenicity of a haplotype can be determined by skin transplantation between siblings. Some haplotypes are rapidly reacted against, others elicit little effect alone but may be effective in combination. A possible recombinant was recognized serologically. The hypothesis was strengthened by the rejection times of skin grafts. Guidelines are suggested for the confirmation of recombination by (a) skin grafting and (b) the production of antibodies against the recombinant haplotypes, and for starting a linkage map.

Cellular and humoral factors governing canine mixed lymphocyte cultures after renal transplantation: III. Further studies with antibody

Further studies have been performed on the unidirectional mixed lymphocyte culture (MLC) in dogs after renal transplantation. A high sensitivity of this assay as a test for antibody was demonstrated. Specific inhibitory activity against donor cells was found in recipient sera beginning 5 days after transplantation and was also present in eluates obtained from rejecting kidneys removed at this time. No cytotoxic antibody was found in the serum or eluates at this time using the microcytotoxicity test. The duration of serum MLC inhibitory activity (in months) correlated with the time in days (2–14) that the allograft was allowed to remain in the recipient. Absorption with donor spleen cells removed MLC inhibitory activity. Finally, a discriminatory test for the presence of blocking antibody in contrast to cytotoxic antibody is described.