Hayrettin Kendir

In vItro plAnT regenerATIon froM HungArIAn veTcH ( vIcIa pannonIca crAnTz) usIng coTyledonAry node explAnTs

ABSTRACT Hungarian vetch is an important forage plant of Southern Europe and South Western Asia. It is progressively gaining popularity as forage plant among farmers of Turkey. In vitro plantlet regeneration in the plant was achieved from cotyledonary node explants on MS medium containing various concentrations of TDZ. The results showed that TDZ concentrations affected frequency of shoot regeneration, mean number of shoots per explants and shoot length in the range of 66.67–100%, 3.28–9.33 and 1.28–3.84 cm respectively. The maximum number of shoots was recorded on MS medium containing 0.45 mg/l TDZ. These were rooted by pulse treatment with 50 mg/l IBA for 7 min and then culturing on 1/2 concentration of MS micro and macro salts and vitamins or MS medium containing 0.30, 0.60, 0.90, 1.20 or 1.60 mg/l IBA. Rooting was more successful on non pulse treated shoots. All of them were put into pots, transferred to growth chamber and then to fields, where they flowered and set seeds.

In vitro regeneration of Turkish dwarf chickling ( Lathyrus cicera L ) using immature zygotic embryo explant

Dwarf chickling (Lathyrus cicera L) is an important forage legume crop. However, due to the presence of neurotoxin amino acid (b-N-oxalyl-L-a,b-diaminopropionic acid) in the dwarf chickling, its cultivation is limited. There is need to carry out studies for the development of varieties with reduced or none of this neurotoxin for beneficial and prolong consumption by animals. The study reports tissue culture approaches for multiplication of the plant using thidiazuron (TDZ), 6-benzylaminopurine (BAP), and �naphthalene acetic acid (NAA) from immature zygotic embryos of the plant. The highest number (16.25) of shoots per explant was recorded on MS medium supplemented with 0.45 mg/l TDZ and 0.4 mg/l ascorbic acid. Much reduced shoot regeneration was recorded on MS medium containing different combinations BAP+NAA+ascorbic acid. The TDZ-regenerated shoots were rooted by pulse treatment with 50 mg/l IBA for 7 min. The plantlets were successfully acclimatized under the greenhouse conditions.

In Vitro Plant Regeneration from Turkish Grasspea (Lathyrus Sativus L.) Using Immature Zygotic Embryo Explant

ABSTRACT Grasspea (Lathyrus sativus L.) is an important grain legume crop cultivated in many countries of the world as animal feed. There is need to improve the plant through various available techniques. The study is aimed to develop an efficient and reproducible protocol for in vitro rapid and large-scale propagation of the plant from immature zygotic embryos using various concentrations of Thidiazuron (TDZ) and 6 benzylaminopurine (BAP)- α Naphthalene acetic acid (NAA) with and without ascorbic acid. The results showed that both TDZ and BAP-NAA without ascorbic acid were ineffective to induce shoot regeneration from explants due to excretion of phenolic compounds. The TDZ (with ascorbic acid) was more potent for axillary shoot regeneration compared to BAP-NAA (with ascorbic acid) with the highest shoot regeneration on MS medium containing 0.45 mg/l TDZ. The shoots regenerated on MS medium containing 0.45 mg/l TDZ (with ascorbic acid) were rooted on MS medium supplemented with 0.30, 0.60, 0.90 and 1.20 mg/l NAA. The best rooting was achieved on MS medium containing 0.90 mg/l NAA. It was not difficult to acclimatize all of the rooted plants in soil in greenhouse.