Helen F Dallas

Critical Thermal Maxima of aquatic macroinvertebrates: towards identifying bioindicators of thermal alteration

Water temperature is an important abiotic driver of aquatic ecosystems. It influences many aspects of an organism’s existence including its growth, feeding and metabolic rates; emergence; fecundity; behaviour and ultimately survival. All organisms have an optimum temperature range within which they survive and are able to thrive. Determining upper thermal limits provides insight into the relative sensitivity of organisms to elevated temperatures. Thermally sensitive taxa may be useful as bioindicators of thermal alteration and used in the generation of target thermal thresholds for aquatic systems. This study determined the upper thermal limit (CTmax) of a range of aquatic macroinvertebrates from rivers in the south-western Cape, South Africa, using the dynamic Critical Thermal Method. The study focused on the taxonomic level of family as an initial screening tool for ranking thermal sensitivity. Of the 27 families examined, four were both thermally sensitive and highly suitable as test organisms, including Paramelitidae, Notonemouridae, Teloganodidae and Philopotamidae. Five families were moderately sensitive and highly suitable, including Palaemonidae, Heptageniidae, Leptophlebiidae, Corydalidae and Aeshnidae. Preliminary experiments to determine potential sources of variation in CTmax revealed that thermal sensitivity was relatively uniform within families, but that acclimation temperature influenced CTmax. Further investigation of the influence of thermal history, acclimation temperature and rate of temperature change on CTmax is necessary. Target water temperatures for river management will be derived using CTmax data, in addition to longer duration experimental data, which will be linked to in situ temperature data.

Bioassessment of the ecological integrity of river ecosystems using aquatic macroinvertebrates: an overview with a focus on South Africa

An overview is given of the bioassessment of the ecological integrity of river ecosystems using aquatic macroinvertebrates, focussing on the South African situation within a global context. Biotic indices and their use in aquatic bioassessment are covered, and comparative descriptions of the more important and/or widely used macroinvertebrate-based biotic indices are given. For each biotic index described, information is provided on the biotopes sampled, the sampling equipment used, the sampling protocol followed, the level of taxonomic resolution, whether identifications are laboratory- or field-based, the range of the final index value, and its current usage. A discussion on using biotic indices and interpreting bioassessment data follows. The multimetric and multivariate approaches to bioassessment are compared, as are the regional and multivariate approaches to the derivation of reference conditions. The use of invertebrate habitat assessment in river bioassessment studies is briefly discussed and the South African spatial framework for analysing bioassessment data is described. In conclusion, a number of potential avenues for further research regarding the use of macroinvertebrates in the bioassessment of river ecosystems are identified.

A comparison of rapid bioassessment protocols used in 2 regions with Mediterranean climates, the Iberian Peninsula and South Africa

Abstract The ability of 2 Rapid Bioassessment Protocols (RBPs) to assess stream water quality was compared in 2 Mediterranean-climate regions. The most commonly used RBPs in South Africa (SA-protocol) and the Iberian Peninsula (IB-protocol) are both multihabitat, field-based methods that use macroinvertebrates. Both methods use preassigned sensitivity weightings to calculate metrics and biotic indices. The SA- and IB-protocols differ with respect to sampling equipment (mesh size: 1000 μm vs 250–300 μm, respectively), segregation of habitats (substrate vs flow-type), and sampling and sorting procedures (variable time and intensity). Sampling was undertaken at 6 sites in South Africa and 5 sites in the Iberian Peninsula. Forty-four and 51 macroinvertebrate families were recorded in South Africa and the Iberian Peninsula, respectively; 77.3% of South African families and 74.5% of Iberian Peninsula families were found using both protocols. Estimates of community similarity compared between the 2 protocols were >60% similar among sites in South Africa and >54% similar among sites in the Iberian Peninsula (Bray–Curtis similarity), and no significant differences were found between protocols (Multiresponse Permutation Procedure). Ordination based on Non-metric Multidimensional Scaling grouped macroinvertebrate samples on the basis of site rather than protocol. Biotic indices generated with the 2 protocols at each site did not differ. Thus, both RBPs produced equivalent results, and both were able to distinguish between biotic communities (mountain streams vs foothills) and detect water-quality impairment, regardless of differences in sampling equipment, segregation of habitats, and sampling and sorting procedures. Our results indicate that sampling a single habitat may be sufficient for assessing water quality, but a multihabitat approach to sampling is recommended where intrinsic variability of macroinvertebrate assemblages is high (e.g., in undisturbed sites in regions with Mediterranean climates). The RBP of choice should depend on whether the objective is routine biomonitoring of water quality or autecological or faunistic studies.

Seasonal variability of macroinvertebrate assemblages in two regions of South Africa: implications for aquatic bioassessment

Seasonal variability of macroinvertebrate assemblages was examined in two regions of South Africa: Western Cape and Mpumalanga. Sampling was undertaken at reference sites using the rapid bioassessment method SASS4 (South African Scoring System, Version 4). This study examined the influence of sampling season on the occurrence of taxa, macroinvertebrate assemblages and SASS scores. In the Western Cape a few taxa were more common at certain times of the year, and multivariate analysis of macroinvertebrate assemblages showed that assemblages grouped by season. The number of taxa and the Average Score per Taxon (ASPT) were significantly different among seasons in the Western Cape, with fewer taxa recorded in winter compared to summer, and significantly higher ASPT values recorded in winter and spring than summer and autumn. Whereas more taxa were recorded in autumn than in spring, a higher proportion of sensitive and high-scoring taxa were recorded in spring. In Mpumalanga macroinvertebrate assemblages did not group by season, and differences in SASS scores were not significant, although more taxa were recorded in winter than in spring or autumn, and ASPTs were slightly higher in winter than in spring. Results are discussed in relation to ecological reference conditions and the interpretation of bioassessment data.

Spatial variability in macroinvertebrate assemblages: comparing regional and multivariate approaches for classifying reference sites in South Africa

Spatial variability in macroinvertebrate assemblages was examined with the aim of evaluating the utility of regional classification systems in aquatic bioassessment. Sampling was undertaken at reference sites in the Western Cape and Mpumalanga, South Africa, using the rapid bioassessment method SASS4 (South African Scoring System Version 4). Multivariate analysis of macroinvertebrate assemblages showed that assemblages varied regionally with differences most apparent in upland areas, i.e. mountain streams and foothill-cobble beds, with lowland areas less regionally distinct. Within regions, longitudinal zonation into upland and lowland areas was important, with sites grouping on the basis of broad geomorphological zones or subregions. Of the upland sites, differentiation into mountain streams and foothill-cobble beds was not evident, although overall variability of assemblages within upland areas, in particular the Western Cape, was very high. In general, a priori regional classification of sites using the hierarchical spatial framework developed in South Africa provided a useful framework for preliminary classification of reference sites. Groups of sites based on a posteriori analysis of macroinvertebrate data, however, provided a more robust classification than any regional classification. Spatial classifications therefore offer geographic partitions within which to expect somewhat similar conditions, and regional reference sites selected within the context of the spatial framework are likely to be more representative of specific river types than those selected without using the framework. Classification of sites needs to be an iterative process that allows for subjective a priori regional classifications to be modified on the basis of independent, objective a posteriori classification of biological assemblages.

The effect of biotope-specific sampling for aquatic macroinvertebrates on reference site classification and the identification of environmental predictors in Mpumalanga, South Africa

The classification of reference sites based on macroinvertebrate assemblages sampled in different aquatic biotopes in Mpumalanga, South Africa, was examined. Environmental variables that best predicted group membership for each of four classifications (stones, vegetation, sand and these three biotopes combined) were identified. Variables at several scales, including catchment, site and habitat, contributed to observed spatial patterns in macroinvertebrate assemblages. Altitude and longitude were important for combined, stones, and vegetation classifications, reflecting the importance of longitudinal zonation and geographic location, respectively. The characteristics of the substratum, including type (bedrock rapid versus cobble riffle), depth, and quality (deposition of silt on stones), were important at habitat scale. Canopy cover was identified as an important predictor in the stones classification. Geological type, which affects overall water chemistry, was important in the stones and combined classification, and temperature was important in the combined classification. Whilst this study is based on correlative data only, it demonstrates that the structure of macroinvertebrate assemblages is a function of both large-scale variables measured at catchment scale and smaller-scale variables measured at site and habitat scale. The study highlights the importance of measuring environmental variables within bioassessment programmes, especially when classifying and assessing reference sites.

Preliminary testing of the Integrated Habitat Assessment System (IHAS) for aquatic macroinvertebrates

Preliminary testing of the Integrated Habitat Assessment System (IHAS), a widely-used aquatic macroinvertebrate habitat assessment method in South Africa, was undertaken. Based on the sensitivity of the South African Scoring System (SASS) to biotope availability and assuming that SASS Scores at minimally-impacted reference sites are the highest Scores attainable, one would expect to find a positive relationship between SASS Scores and IHAS scores at reference sites. The preliminary testing undertaken is based on the assumption that this relationship should be linear. For data obtained from reference sites in Mpumalanga and the Western Cape, non-parametric correlation analyses were undertaken between SASS4/5 Scores and IHAS scores, using Kendalls Rank-correlation Coefficient. Separate analyses were undertaken for different geomorphological zones and biotope groups. Results were generally unsatisfactory, with non-significant correlations (P > 0.05) for two-thirds of the data sets analysed. Performance of the IHAS varied between geomorphological zones and biotope groups, with best results obtained for the Foothill: Gravel-bed Zone in Mpumalanga, particularly with the stones-in-current biotope analysed separately. Further testing is required to confirm the relative performance in different bioregions/ecoregions, geomorphological zones and biotope groups.