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Dive into the research topics where Helen Rees is active.

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Featured researches published by Helen Rees.


Extremophiles | 2004

Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods

Helen Rees; William D. Grant; Brian E. Jones; Shaun Heaphy

DNA was extracted from water and sediment samples taken from soda lakes of the Kenyan-Tanzanian Rift Valley. DNA was also extracted from microbial enrichment cultures of sediment samples. 16S rRNA genes were amplified by the polymerase chain reaction and microbial diversity was studied using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA amplicons. Cloning and sequencing of single DGGE bands showed that they usually contained mixed amplicons. Several of the amplicon sequences had high identities, up to 99%, with 16S rRNA genes of organisms previously isolated from soda lakes, while others were only distantly related, with identities as low as 82%. Phylogenetic analysis of the sequenced amplicons indicated that sequences were related to the haloarchaeal, Bacillus/Clostridium, Rhodobacterium/Thioalcalovibrio/ Methylobacter, and Cytophaga/Flavobacterium/Bacteroides (CFB) groups and the enterobacteria/Aeromonas/Vibrio part of the γ3 subdivision of the Proteobacteria.


Extremophiles | 2003

Detecting cellulase and esterase enzyme activities encoded by novel genes present in environmental DNA libraries

Helen Rees; Susan Grant; Brian E. Jones; William D. Grant; Shaun Heaphy

A genomic DNA library was made from the alkaliphilic cellulase-producing Bacillus agaradhaerans in order to prove our technologies for gene isolation prior to using them with samples of DNA isolated directly from environmental samples. Clones expressing a cellulase activity were identified and sequenced. A new cellulase gene was identified. Genomic DNA libraries were then made from DNA isolated directly from the Kenyan soda lakes, Lake Elmenteita and Crater Lake. Crater Lake clones expressing a cellulase activity and Lake Elmenteita clones expressing a lipase/esterase activity were identified and sequenced. These were encoded by novel genes as judged by DNA sequence comparisons. Genomic DNA libraries were also made from laboratory enrichment cultures of Lake Nakuru and Lake Elmenteita samples. Selective enrichment cultures were grown in the presence of carboxymethylcellulose (CMC) and olive oil. A number of new cellulase and lipase/esterase genes were discovered in these libraries. Cellulase-positive clones from Lake Nakuru were isolated at a frequency of 1 in 15,000 from a library made from a CMC enrichment as compared to 1 in 60,000 from a minimal medium enrichment. Esterase/lipase-positive clones from Lake Elmenteita were isolated with a frequency of 1 in 30,000 from a library made from an olive-oil enrichment as compared to 1 in 100,000 from an environmental library.


Archive | 2004

Novel Lipolytic Enzyme ELIP

Brian E. Jones; William D. Grant; Shaun Heaphy; Susan Grant; Helen Rees


Archive | 2009

Lipolytic enzyme ELIP

Brian E. Jones; William D. Grant; Shaun Heaphy; Susan Grant; Helen Rees


Archive | 2012

LIPOLYTIC ENZYME LIP1

Brian E. Jones; William D. Grant; Shaun Heaphy; Susan Grant; Helen Rees


Archive | 2010

***WITHDRAWN PATENT AS PER THE LATEST USPTO WITHDRAWN LIST***Lipolytic enzyme LIP2

Brian E. Jones; William D. Grant; Shaun Heaphy; Susan Grant; Helen Rees


Archive | 2010

Lipolytic enzyme LIP2

Brian E. Jones; William D. Grant; Shaun Heaphy; Susan Grant; Helen Rees


Proceedings of International Symposium on Extremophiles and Their Applications International Symposium on Extremophiles and Their Applications 2005 | 2007

Cloning Microbial Metagenomes

Shaun Heaphy; Susan Grant; Alan J. Cann; Helen Rees; Bill Grant


Archive | 2004

Une nouvelle enzyme lipolytique dite elip

Brian E. Jones; William D. Grant; Shaun Heaphy; Helen Rees; Susan Grant


Archive | 2004

Nouvelle enzyme lipolytique lip2

Brian E. Jones; William D. Grant; Shaun Heaphy; Helen Rees; Susan Grant

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Susan Grant

University of Leicester

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Alan J. Cann

University of Leicester

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