Hendrik R. Stapert

Anke Pierik; M Moamfa; M. van Zelst; D. Clout; Hendrik R. Stapert; Johan Frederik Dijksman; D. Broer; Reinhold Wimberger-Friedl

Quantitative real-time polymerase chain reaction (qrtPCR) is widely used as a research and diagnostic tool. Notwithstanding its many powerful features, the method is limited in the degree of multiplexing to about 6 due to spectral overlap of the available fluorophores. A new method is presented that allows quantitative amplification detection at higher multiplexing by the integration of amplification in solution and monitoring via hybridization to a microarray in real-time. This method does not require any manipulation of the PCR product and runs in a single closed chamber. Employing labeled primers, one of the main challenges is to measure surface signals against a high fluorescence background from solution. A compact, confocal scanner is employed, based on miniaturized optics from DVD technology and combined with a flat thermocycler for simultaneous scanning and heating. The feasibility of this method is demonstrated in singleplex with an analytical sensitivity comparable to routine qrtPCR.

Nobuhiro Nishiyama; Hendrik R. Stapert; Guo Dong Zhang; Daisuke Takasu; Dong Lin Jiang; Tetsuo Nagano; Takuzo Aida; Kazunori Kataoka

Hendrik R. Stapert; Nobuhiro Nishiyama; Dong Lin Jiang; Takuzo Aida; Kazunori Kataoka

Derk Jan Wilfred Klunder; Maarten Marinus Johannes Wilhelm Van Herpen; Hendrik R. Stapert

Erik Robbert Vossenaar; Marc Wilhelmus Gijsbert Ponjee; Hendrik R. Stapert; Mark Thomas Johnson

Reinhold Wimberger-Friedl; Hendrik R. Stapert

Emile Johannes Karel Verstegen; Arjen Gerben Van Der Sijde; Edwin Maria Wolterink; Hendrik R. Stapert

Marcel Rene Boehmer; Hendrik R. Stapert; Paulus Cornelis Duineveld

Helmar Van Santen; Hendrik R. Stapert; Johan George Kloosterboer

Emile Johannes Karel Verstegen; Hendrik R. Stapert