Herman Bernitz

An Integrated Technique for the Analysis of Skin Bite Marks

Abstract: The high number of murder, rape, and child abuse cases in South Africa has led to increased numbers of bite mark cases being heard in high courts. Objective analysis to match perpetrators to bite marks at crime scenes must be able to withstand vigorous cross‐examination to be of value in conviction of perpetrators. An analysis technique is described in four stages, namely determination of the mark to be a human bite mark, pattern association analysis, metric analysis and comparison with the population data, and illustrated by a real case study. New and accepted techniques are combined to determine the likelihood ratio of guilt expressed as one of a range of conclusions described in the paper. Each stage of the analysis adds to the confirmation (or rejection) of concordance between the dental features present on the victim and the dentition of the suspect. The results illustrate identification to a high degree of certainty.

A Technique to Capture, Analyze, and Quantify Anterior Teeth Rotations for Application in Court Cases Involving Tooth Marks

ABSTRACT: Anterior teeth within the human dentition have a specific numerical rotation value. Bite marks show an array of angled indentations, abrasions, microlacerations, and contusions. These marks generally represent the incisal surfaces of the suspects dentition reflecting the rotation values of the teeth in the dental arch. This study described a method for capturing and analyzing anterior dental rotations. The rotations of individual anterior teeth within the study population were categorized as common, uncommon, and very uncommon according to Allens classification. In the absence of a large number of incisal patterns present in a bite mark, a single but heavily weighted tooth rotation could be of equal discriminatory potential to several common rotation values. No prevalence studies quantifying individual tooth rotations are available. The measurement of each individual tooth rotation together with its individual discrimination potential will enhance the evaluation of the concordant features observed in bite marks.

Flow cytometric evaluation of postmortem pulp DNA degradation.

During postmortem autolysis, cellular organelles and nuclear DNA break down into their constituent parts. DNA flow cytometric analysis was applied to study the denaturation of splenic cell DNA as a possible method for postmortem interval determination. DNA denaturation continued for 72 hours at a constant rate, with no intact DNA peaks thereafter. The value of using dental pulp tissue for flow cytometric determination of postmortem interval was investigated. The pulps of 57 routinely removed impacted third molars from patients 15 to 30 years of age were obtained. Pulp tissue was removed at 24, 48, 72, 96, 120, and 144 hours postextraction. Debris (degraded DNA) was defined as all signals left of the standardized mean 2 n peak and expressed as a percentage of the total number of signals. In contrast to the splenic cell DNA, dental pulp tissue exhibited minimal DNA degradation by 144 hours postextraction, and no constant relation was found between time and DNA degradation during this time. In this study, pulp tissue was found to be unreliable to determine the early postmortem interval but might be of greater value in the later stages.

Erratum to: The individualisation of a dog bite mark: a case study highlighting the bite mark analysis, with emphasis on differences between dog and human bite marks

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Reconstruction of Deformed Bite Marks Using Affine Transformations

Abstract:  Bite marks inflicted on animate and inanimate objects will undergo a certain degree of deformation. This fact remains one of the biggest stumbling blocks when analyzing evidence for court presentation. It has been demonstrated that the reliability of pattern association analysis will not be affected by minimal degrees of warping, shrinkage, and distortion. In this study, affine transformations were applied to bite marks to establish if minimal distortions would affect the mathematically determined relationships of the defined features. In a real case study, it was then tested whether the distorted bite mark matched the dentition of the perpetrator by applying an affine transformation. This was confirmed to be the case. Affine transformations will thus not affect the relationships of the individual features found in bite marks. The numerical calculations validate the reliability of pattern association analysis in the presence of minimal amounts of warping, shrinkage, and distortion.

The application of affine transformations in matching distorted forensic samples with a common origin

The practice of forensic odontology regularly requires the forensic dental expert to establish a degree of concordance between two objects, one or both of which have undergone minimal degrees of warping, shrinkage and distortion collectively described as deformations. These comparisons can be between the suspects or victims dentition and bite marks on inanimate objects/skin, two radiological images, palatal rugae patterns, dental arches, lip prints and other unique scenarios which are presented in forensic odontological cases. This paper will define the mathematical concept of affine transformation as it pertains to forensic matters and explains how it can be applied to a case study involving pattern deformations. Although the deformation of the evidence may be minimal in extent, it creates a measure of uncertainty when expert evidence is given in court cases. Any metric discrepancy caused by deformation of the evidence will necessitate the application of pattern association and negate the use of metric analysis. It has been shown that a pattern association analysis of evidence will not be affected by minimal amounts of deformation. The mathematical limits of these deformations which will significantly affect the comparisons have not yet been determined. In the case study presented the deformations were considered minimal and thus explainable by a pattern association analysis. The mathematical concepts will empower the expert to explain to a court of law how two samples which were not a perfect match, could in fact be deformed equivalents of common origin.

Reply to letter received from Prof Michael Bohnert regarding article on “Tongue protrusion as an indicator of vital burning”

In the forensic assessment of burned bodies, the question of whether the victim was exposed to the fire before or after death is of crucial importance. Several external signs have been used in the past to indicate heat exposure prior to the death of the victim but these did not include tongue protrusion. The internal signs of heat exposure are generally regarded as much more important than those observed externally. No one factor has been found to be totally reliable. This study found a statistically significant dependence between tongue protrusion and presence of soot in the respiratory tract and stomach, the latter being a good indicator of vital burning. The results of this study confirm that tongue protrusion can be used as an additional indicator of vital burning.

Legal Aspects Related to Healthcare Insurance, Dental Practitioners, and Forensic Odontologists in South Africa

Healthcare insurance providers, dental practitioners, and forensic odontologists are highly regulated in South Africa. Healthcare providers are regulated by the Council for Medical Schemes, CMS (Council for Medical Schemes), while dental practitioners and forensic odontologists are regulated by the Health Professions Council of South Africa (HPCSA) (Health Professions Council of South Africa).

Louse (Insecta: Phthiraptera) infestations of the Amur Falcon (Falco amurensis) and the Red-footed Falcon

Abstract Little is known about the louse species harboured by Red-footed and Amur Falcons despite the fact that various life-history traits of these hosts make them good model species to study host-parasite interactions. We collected lice samples from fully grown Amur (n=20) and Red-footed Falcons (n=59), and from nestlings of Red-footed Falcons (n=179) in four countries: Hungary, India, Italy and South Africa. We identified 3 louse species on both host species, namely Degeeriella rufa, Colpocephalum subzerafae and Laembothrion tinnunculi. The latter species has never been found on these hosts. Comparing population parameters of lice between hosts we found significantly higher prevalence levels of D. rufa and C. subzerafae on Amur Falcons. Adult Red-footed Falcons had higher D. rufa prevalence compared to C. subzerafae. For the first time we also show inter-annual shift in prevalence and intensity levels of these species on Red-footed Falcons; in 2012 on adult hosts C. subzerafae had higher intensity levels than D. rufa, however in 2014 D. rufa had significantly higher intensity compared to C. subzerafae. In case of nestlings both louse species had significantly higher preva lence levels than in 2014. The exact causes of such inter-annual shifts are yet to be understood.