Hh Gerhartz

Effect of low-dose whole-body irradiation on granulopoietic progenitor cell subpopulations: implications for CFUc release

Abstract. The reaction of the granulopoietic system to whole body irradiation with 0.80 and 1.60 Gy was studied in dogs by means of colony formation assays in combination with velocity sedimentation and tritiated thymidine cytocidal techniques. Depression of circulating CFUc was associated with a marked shift in the size distribution of granulopoietic progenitors in the bone marrow. This effect lasted much longer in those animals receiving the lower X‐ray dose. We conclude that circulating CFUc are not a random proportion of the bone marrow but a subpopulation of cells which are smaller in size. These cells are in an equilibrium with the larger marrow CFUc, which is very sensitive to perturbations.

Granulozytär determinierte Stammzellen (CFU-C) aus Knochenmark und Blut: Größenunterschiede vor und nach Dextransulfat-Mobilisation

SummaryGranulopoietic progenitor cells (CFU-C) from beagle bone marrow and blood were compared by velocity sedimentation separation. Blood CFU-C are significantly smaller than their bone marrow counterparts. Mobilization by dextran sulfate leads to release of only small CFU-C, indicating the existence of a pool of CFU-C which are ready to enter the peripheral circulation.

GRANULOPOIETIC PROGENITOR CELLS (CFU-C) IN BONE-MARROW AND BLOOD - DIFFERENCE IN SIZE AND INFLUENCE OF MOBILIZATION BY DEXTRAN SULFATE

SummaryGranulopoietic progenitor cells (CFU-C) from beagle bone marrow and blood were compared by velocity sedimentation separation. Blood CFU-C are significantly smaller than their bone marrow counterparts. Mobilization by dextran sulfate leads to release of only small CFU-C, indicating the existence of a pool of CFU-C which are ready to enter the peripheral circulation.

Granulozytär determinierte Vorläuferzellen (CFU-C) und hämopoetisches Potential: Eine Studie im Hundemodell

SummaryFour beagles received a standardized dose of 33000–44000 CFU-C per kg b.w. isolated from the peripheral blood of DLA-identical donors. Three had permanent reconstitution of hemopoiesis while in one regeneration was abortive. This indicates that the CFU-C assay is able to predict for the hemopoietic potential of a graft. However, considerable differences in the regeneration pattern of CFU-C in the bone marrow demonstrated that the number of repopulating stem cells was variable.Four beagles received a standardized dose of 33000-44000 CFU-C per kg b.w. isolated from the peripheral blood of DLA-identical donors. Three had permanent reconstitution of hemopoiesis while in one regeneration was abortive. This indicates that the CFU-C assay is able to predict for the hemopoietic potential of a graft. However, considerable differences in the regeneration pattern of CFU-C in the bone marrow demonstrated that the number of repopulating stem cells was variable.

Granulopoietic progenitor cells and the hematopoietic potential of a graft - A study in the dog-model

SummaryFour beagles received a standardized dose of 33000–44000 CFU-C per kg b.w. isolated from the peripheral blood of DLA-identical donors. Three had permanent reconstitution of hemopoiesis while in one regeneration was abortive. This indicates that the CFU-C assay is able to predict for the hemopoietic potential of a graft. However, considerable differences in the regeneration pattern of CFU-C in the bone marrow demonstrated that the number of repopulating stem cells was variable.Four beagles received a standardized dose of 33000-44000 CFU-C per kg b.w. isolated from the peripheral blood of DLA-identical donors. Three had permanent reconstitution of hemopoiesis while in one regeneration was abortive. This indicates that the CFU-C assay is able to predict for the hemopoietic potential of a graft. However, considerable differences in the regeneration pattern of CFU-C in the bone marrow demonstrated that the number of repopulating stem cells was variable.

Granulozytr determinierte Vorluferzellen (CFU-C) und hmopoetisches Potential: Eine Studie im Hundemodell@@@Granulopoietic progenitor cells and the hemopoietic Potential of a graft: a study in the dog model

SummaryFour beagles received a standardized dose of 33000–44000 CFU-C per kg b.w. isolated from the peripheral blood of DLA-identical donors. Three had permanent reconstitution of hemopoiesis while in one regeneration was abortive. This indicates that the CFU-C assay is able to predict for the hemopoietic potential of a graft. However, considerable differences in the regeneration pattern of CFU-C in the bone marrow demonstrated that the number of repopulating stem cells was variable.Four beagles received a standardized dose of 33000-44000 CFU-C per kg b.w. isolated from the peripheral blood of DLA-identical donors. Three had permanent reconstitution of hemopoiesis while in one regeneration was abortive. This indicates that the CFU-C assay is able to predict for the hemopoietic potential of a graft. However, considerable differences in the regeneration pattern of CFU-C in the bone marrow demonstrated that the number of repopulating stem cells was variable.