Hidemi Hosogi | Researchain

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Experimental Animals | 1978

Measurement of Rat Serum Corticosterone Using Radiostereoassay Kit for Cortisol

Hidemi Hosogi; Toyoko Uneki; Jiro Takahara; Tadashi Ofuji

A very simple technique for measurement of rat serum corticosterone with radiostereoassy kit using 75Se-cortisol was devised. Only 25--50 microliter serum was required for this assay. The serum sample was first diluted to 300 microliter with water and heated in a glass tube to denature the endogeneous cortisol binding protein. Then an aliquot of the sample was transferred into an assay vial containing 75Se-cortisol, cortisol binding protein and adsorbent granules. After rotated, an aliquot of the supernatant was taken for counting. High level of rat serum corticosterone was measured with satisfying accuracy by dilution with water. Serum corticosterone levels in 7 normal female Sprague-Dawley rats (7 weeks old, Ca 200g body weight) at 2.00 pm were 29.5 +/- 12.2 microgram/dl (mean +/- SD). The levels were lower and higher in rats treated with dexamethasone and with 1-18 ACTH, respectively.

Endocrinology | 1980

Concomitant Increases in Serum Growth Hormone and Hypothalamic Somatostatin in Rats after Injection of γ-Aminobutyric Acid, Aminooxyacetic Acid, or γ-Hydroxybutyric Acid

Jiro Takahara; Sho Yunoki; Hidemi Hosogi; Wataru Yakushiji; Jingo Kageyama; Tadashi Ofuji

Endocrinologia Japonica | 1976

Hypothalamic pituitary adrenal function in patients with anorexia nervosa.

Jiro Takahara; Hidemi Hosogi; Sho Yunoki; Kozo Hashimoto; Toyoko Uneki; Tadashi Ofuji

Endocrinologia Japonica | 1977

Effects of prostaglandin E1 and indomethacin on ACTH, prolactin, GH and LH from rat pituitary in vitro.

Jiro Takahara; Masahiko Mori; Nobuko Ofuji; Kozo Hashimoto; Jiro Yamauchi; Yukio Yamane; Hidemi Hosogi; Tadashi Ofuji

Endocrinologia Japonica | 1976

Plasma immunoassayable ACTH levels during and after hydrocortisone infusion in patients with Cushing's disease.

Kozo Hashimoto; Jiro Takahara; Hidemi Hosogi; Sho Yunoki; Tadashi Ofuji

Acta Medica Okayama | 1979

Specificity of cultured anterior pituitary cells in detecting corticotropin releasing factor(s): the effect of biologically active peptides and neurotransmitter substances on ACTH release in pituitary cell cultures.

Kozo Hashimoto; Sho Yunoki; Hidemi Hosogi; Jiro Takahara; Tadashi Ofuji

The Journal of Clinical Endocrinology and Metabolism | 1977

Transient appearance of a provocative growth hormone response to L-dopa following incomplete adenomectomy in an acromegalic patient.

Jiro Takahara; Wataru Yakushiji; Jiro Yamauchi; Masanori Miyoshi; Hidemi Hosogi; Tadashi Ofuji

Internal Medicine | 1995

Non-Insulin-Dependent Diabetes Mellitus Complicated with Idiopathic Hypoparathyroidism

Hiroyuki Itoh; Yuzo Matsuda; Mitsuru Nishiyama; Takao Kitagawa; Tomoko Kobayashi; Yutaka Nio; Satoshi Hama; Hidemi Hosogi; Tadashi Hiroi; Kozo Hashimoto

Endocrinologia Japonica | 1975