Hideo Bando

Two new diterpenoid alkaloids from Delphinium pacific giant and revised 13C-NMR assignment of delpheline

Two new C 19 -diterpenoid alkaloids, paciline and pacinine, and a known alkaloid, delpheline were isolated from Delphinium pacific giant. Structures of those alkaloids were determined on the basis of their spectral data and chemical correlation with delpheline

Determination and quantitative analysis of Aconitum alkaloids in plants by liquid chromatography—atmospheric pressure chemical ionization mass spectrometry

Abstract A high-performance liquid chromatography—atmospheric pressure chemical ionization mass spectrometry method was useful for the simultaneous determination of Aconitum alkaloids, kobusine, pseudokobusine, dehydrolucidusculine, lucidusculine, delcosine and 14-acetyldelcosine, found in the Aconitum yesoense var. macroyesoense. These compounds were chromatographed by definite elution in 10 min and were quantitated by selected-ion monitoring of the protonated molecules. The method is linear over the range 10 ng to 10 μg of alkaloids per injection. The detection limits of alkaloids were ca. 1–5 ng per injection.

Studies on the constituents of aconitum species. VII: On the components of Aconitum japonicum Thunb.

Two new diterpenoid alkaloids, subcumine and subcusine, were isolated from the roots of Aconitum japonicum Thunb. The structures of these alkaloids were determined on the basis of spectral data, chemical evidence, and X-ray analysis. We also have isolated two known alkaloids, ezochasmaconitine and anisoezochasmaconitine

Four diterpenoid alkaloids from Delphinium elatum

Abstract Four new C 19 -diterpenoid alkaloids, pacifidine, pacifiline, pacifinine and pacidine, and five known alkaloids were isolated from Delphinium elatum cv. Pacific Giant. The structures of these alkaloids were determined on the basis of their spectral data.

Studies on Aconitum species. XI: Two new diterpenoid alkaloids from Aconitum yesoense var. Macroyesoense (Nakai) Tamura. V

A new C 20 -diterpenoid alkaloid, yesonine, a new C 19 -diterpenoid alkaloid, α-oxobrowniine, and five known alkaloids were isolated from Aconitum yesoense var. macroyesoense (Nakai) Tamura. Structures of those new alkaloids were determined on the basis of their spectral and chemical correlation with known alkaloids. Hydrolysis of yesoline and permanganate oxidation of browniine afforded yesonine and α-oxobrowniine, respectively

Guluronate content of alginate in Laminaria japonica and Laminaria angustata fronds in laboratory culture

The guluronate (G) content of alginate in the fronds of Laminaria japonica and Laminaria angustata, cultured in the laboratory from zoospore via gametophyte using PESI medium, was determined by the 1H‐nuclear magnetic resonance spectroscopic method. The G content of alginates in young fronds cultured in various conditions were shown to exceed 55%. These values were remarkably higher than those in field kelp. The G content increased with extending culture period and at low temperature.

Chapter 3 Aconitum Alkaloids

Publisher Summary This chapter provides an overview of the recent developments in the study of alkaloids obtained from Aconitum species. The diterpenoid alkaloids isolated from Aconitum species can be divided into two groups: those based on a hexacyclic C 19 skeleton and those based on a C 20 skeleton. The C 19 diterpenoid alkaloids can be further divided into two types: aconitine-type alkaloids, represented by aconitine, and lycoctonine type alkaloids, represented by lycoctonine. The C 20 diterpenoid alkaloids are divided into atisinetype alkaloids, represented by atisine, and veatchine-type alkaloids, represented by veatchine. Glaucine, an aporphine alkaloid isolated from Aconitum yesoense, is known to have antitussive activity. Isoboldine, obtained from aerial parts of A.karakolicum, possesses antifeeding activity in Trimerisia miranda and Prodenia litura. Several methods, such as UV spectroscopy, paper electrophoresis , thin-layer chromatography , and multibuffered paper partition chromatography are developed for quantitative determination of the toxic alkaloid aconitine.