Hideo Mitsuya

Transperineal 12‐core systematic biopsy in the detection of prostate cancer

The present study was designed to determine the clinical value of transperineal 12‐core systematic prostate biopsy guided by transrectal ultrasonography (TRUS) in the detection of prostate cancer.

Evidence for contractility of the human seminiferous tubule confirmed by its response to noradrenaline and acetylcholine

The experiments reported here demonstrate for the first time that the isolated human seminiferous tubule is capable of undergoing contraction after exposure to noradrenaline and acetylcholine. Isoproterenol produces a relaxation of the seminiferous tubule. It is indicated that there are the adrenergic alpha and beta receptors and muscarinic receptors in the myoid cells of human seminiferous tubules.

An immunochemical and immunohistochemical study of S100 protein in renal cell carcinoma

The authors localized S100 protein in renal tubules and renal cell carcinoma by immunohistochemical study and quantitative analysis by enzyme immunoassay. The α subunit of S100 protein (S100‐α) was localized in epithelial cells of proximal tubules, thin limbs of loops of Henle, collecting tubules, and a few of Bowmans capsules. The β subunit (S100‐β) was immunostained in the distal tubules, thick and thin loops of Henle, collecting tubules, and a few proximal tubules. In renal cell carcinoma, S100‐α was immunohistochemically demonstrated in 82% (41/50) of patients including sarcomatoid variants, whereas S100‐β was detected in 46% (23/50). Both the number of positively stained tumor cells and the staining intensity were greater in S100‐α than in S100‐β. Concentrations of S100‐α in the cortex were 80.3 ± 22.5 ng/mg protein (n = 7), whereas those of renal cell carcinoma were 387 ± 533 ng/mg protein (n = 19), i.e., about five times higher. Concentrations of S100‐β in both normal kidney (1.96 ± 0.74 ng/mg protein) and renal cell carcinoma (2.05 ± 2.16 ng/mg protein) were much lower than those of S100‐α. The authors also localized S100‐α and S100‐β in tissues of other renal tumors and tumors arising from other organs. S100ao appears to be a useful immunohistochemical marker for renal cell carcinoma.

Signet-Ring Cell Carcinoma Involving the Urinary Bladder

A case of signet-ring cell carcinoma involving the urinary bladder, which is a rare variant of adenocarcinoma, is presented. 21 cases have so far been reported in the occidental and Japanese journals.

Children With Unstable Bladder: Clinical and Urodynamic Observation

A clinical analysis was done on 303 patients diagnosed by urodynamic study as having an unstable bladder, which was characterized by uninhibited contraction of the detrusor without any overt neurogenic lesions. An age distribution histogram demonstrated 2 peaks: children less than 10 years old and patients between 60 and 80 years old. Sex was not significant in the children but men predominated in the adults. There were 68 children less than 15 years old with the chief complaints of enuresis (54 per cent), urge incontinence (41 per cent) and fever (25 per cent). The objective findings were vesicoureteral reflux in 29 children, urinary tract infection in 20 and distal urethral stenosis in 8. An unstable bladder type I, the occurrence of uninhibited contractions during the filling phase of cystometry, was found in 53 children (78 per cent). We noted several postures that the children characteristically used to resist unheralded urgency and to prevent urine from leaking. The main therapeutic regimen consisted of anticholinergics with or without antimicrobials. Several children underwent surgical intervention. Once the antireflux operation was performed followup with anticholinergics was of primary importance. The unstable bladders of 63 of the 68 children (93 per cent) have been controlled satisfactorily. The etiology of bladder instability is discussed for both groups of patients.

Intrascrotal extratesticular neurofibroma

Abstract A case of intrascrotal, extratesticular neurofibroma in an eight-year-old boy is reported. These tumors, which arise from perineural and Schwann cells, commonly occur throughout the body but have not been reported previously to originate from the subcutaneous tissue of the scrotum. No stigmas of von Recklinghausen disease are identified. Histopathology of neurofibroma is discussed.

Malignant Fibrous Histiocytoma Arising from the Renal Capsule: Report of a Case

We report a case of malignant fibrous histiocytoma arising from the renal capsule. The tumor was found during screening ultrasonography. Surgical excision and adjuvant chemotherapy consisting of cyclophosphamide, vincristine, adriamycin and actinomycin D were performed. There has been no recurrence as of 2 years and 4 months after the operation. We reviewed the 5 other cases of malignant fibrous histiocytoma arising from the renal capsule.

Crossed Ectopia of Solitary Kidney

Two cases of crossed ectopia of solitary kidney found in two boys are present. This rare anomaly has been accompanied by imperforate anus and inguinal hernia in one boy and by imperforate anus, urethr

Elastic fibers in tunica propria of undescended and contralateral scrotal testes from cryptorchid patients

Elastic fibers in the tunica propria of the testes from cryptorchid patients and normal fertile adults were examined by light (Weigert resorcin-fuchsin stain) and electron microscopic techniques. In the testes from normal fertile adults, elastic fibers were proved to exist in the tunica propria by light and electron microscopy, and were located in the fibrous and cellular layers of the tunica propria. In the undescended testes from the cryptorchid patients, during prepubertal and pubertal periods, elastic fibers could not be visualized in the tunica propria, but were found after puberty. The positive Weigert reaction of the tunica propria in the undescended testes from postpubertal cryptorchid patients, however, was markedly weaker than that in normal control patients, suggesting diminution of elastic fibers. This diminution of elastic fibers in the undescended testes from postpubertal cryptorchid patients was also substantiated by electron microscopy. However, in the contralateral scrotal testes, elastic fibers appeared during puberty and were observed after puberty in the same manner as in normal testes. Thus, the present study suggested retarded appearance of elastic fibers in puberty and impaired development of those fibers after puberty, in the undescended testes of cryptorchid patients.

Leydig cell hyperplasia in cryptorchid patients: Quantitative evaluation of Leydig cells in undescended and contralateral scrotal testes

SummaryLeydig cell number was evaluated quantitatively in testicular biopsies from post-pubertal cryptorchid patients and normal controls. For this quantitative evaluation we used the following method. This is based on the determination of the total number of Leydig cells, Leydig cell clusters and seminiferous tubules in the entire histologic sections of each biopsy and the determination of the following indices; mean Leydig cells per tubule, mean Leydig cell clusters per tubule and mean Leydig cells per cluster. In addition, the numbers of Sertoli cells were counted, and Leydig-Sertoli cell ratio was also determined. These indices were correlated with each other. All indices were significantly elevated not only in undescended but in contralateral scrotal testes of the cryptorchid patients in comparison to those in normal controls. Between undescended and descended scrotal testes of the same individual patients, those indices were significantly higher in the descended scrotal testes than in the undescended ones. Thus, Leydig cell hyperplasia was noted in the testes of post-pubertal cryptorchid patients, and was more prominent in the contralateral scrotal testes than in the undescended ones.