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Cytometry | 2000

High‐throughput pretreatment system in automated urinary sediment analyzer

Ryo Miyake; Isao Yamazaki; Yasuaki Kojima; M. Kurimura; Hideyuki Horiuchi

BACKGROUND Urine contains microscopically observable particles that can indicate certain types of disease in the urinary tract system. Determining these various types of sediments by manual operation is a cumbersome and time-consuming task. To eliminate this labor, we developed an automated urinary sediment analyzer with high-throughput pretreatment system. METHODS The pretreatment system mainly consists of four reaction vessels for dying samples (urine), a sheath flow chamber, and an unique sample carrier mechanism from the reaction vessel to the flow chamber, which enables overlapped processing, and rapid transfer of samples with small dispersion and a short buildup time. RESULTS The buildup time was experimentally found to be 1.8 s, and the extra-sample volume beside that for measurement was only 4.9 microl (1/20 of the total sample volume). CONCLUSIONS Short buildup time results in high throughput of 120 samples per hour, and relatively small extra-volume contributes to reduce carryover.


Archive | 1994

Flow cell apparatus

Masaetsu Matsumoto; Isao Yamazaki; Ryo Miyake; Masaharu Ishii; Ryohei Yabe; Hiroshi Ohki; Hideyuki Horiuchi; Shinichi Sakuraba


Archive | 1989

Analyzing system using sheath flow of sample

Ryo Miyake; Hiroshi Ohki; Isao Yamazaki; Toshio Kaneko; Hideyuki Horiuchi; Shinichi Sakuraba; Kaori Yasuda


Archive | 1994

Flow type particle image analyzing method and apparatus

Hideyuki Horiuchi; Koji Suda; Masaetsu Matsumoto


Archive | 1993

An apparatus for investigating particles in a fluid, and a method of operation thereof

Isao Yamazaki; Hiroshi Ohki; Masaetsu Matsumoto; Ryo Miyake; Ryohei Yabe; Hideyuki Horiuchi; Shinichi Sakuraba


Archive | 1982

Laser surgical equipment

Tamotsu Shimada; Chiaki Shinbo; Hideyuki Horiuchi; Masamoto Takatsuji


Archive | 1997

Method and apparatus for particle image analysis using flow cell

Hideyuki Horiuchi


Archive | 1996

Picture reproducing apparatus

Akira Hashimoto; Hideyuki Horiuchi; Manabu Yamamoto


Archive | 1989

Method and apparatus for analysis of particles contained in a liquid sample

Ryo Miyake; Hiroshi Ohki; Isao Yamazaki; Toshio Kaneko; Hideyuki Horiuchi; Shinichi Sakuraba; Kaori Yasuda


Archive | 1992

Cell analysis apparatus

Hideyuki Horiuchi; Shinichi Sakuraba; Toshio Kaneko; Nobuyuki Tatara; Ryohei Yabe; Hiroshi Ohki; Isao Yamazaki; Ryo Miyake

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