Hideyuki Kobayashi

A Point Mutation of Tyr-759 in Interleukin 6 Family Cytokine Receptor Subunit gp130 Causes Autoimmune Arthritis

We generated a mouse line in which the src homology 2 domain–bearing protein tyrosine phosphatase (SHP)-2 binding site of gp130, tyrosine 759, was mutated to phenylalanine (gp130 F759/F759). The gp130 F759/F759 mice developed rheumatoid arthritis (RA)-like joint disease. The disease was accompanied by autoantibody production and accumulated memory/activated T cells and myeloid cells. Before the disease onset, the T cells were hyperresponsive and thymic selection and peripheral clonal deletion were impaired. The inhibitory effect of IL-6 on Fas ligand expression during activation-induced cell death (AICD) was augmented in gp130 F759/F759 T cells in a manner dependent on the tyrosine residues of gp130 required for signal transducer and activator of transcription 3 activation. Finally, we showed that disease development was dependent on lymphocytes. These results provide evidence that a point mutation of a cytokine receptor has the potential to induce autoimmune disease.

Calcitonin gene related peptide stimulates adenylate cyclase activity in rat striated muscle

Rat calcitonin gene related peptide (CGRP) and salmon calcitonin (CT) stimulated adenylate cyclase activity in a dose-dependent manner in the rat diaphragm and in the kidney. The ED50 value of rat CGRP was lower and that of salmon CT was higher in the diaphragm than in the kidney. These results suggest that CGRP stimulates adenylate cyclase activity in the striated muscle by reacting with sites distinct from the site in the kidney.

Enhanced production of osteopontin in multiple myeloma: clinical and pathogenic implications

Summary. In this study, we examined osteopontin (OPN) production in myeloma cells and plasma OPN levels in multiple myeloma (MM) patients. We assessed OPN production in bone marrow cells (BMCs) by immunocytochemistry and enzyme‐linked immunosorbent assay (ELISA). We also assessed OPN production in various B‐cell malignant cell lines, including three myeloma cell lines by reverse transcription polymerase chain reaction (RT‐PCR) and Western blotting. In addition, we measured plasma OPN concentrations by ELISA in 30 MM patients, 21 monoclonal gammopathy of undetermined significance (MGUS) patients and 30 healthy volunteers. As a result, in an immunocytochemical study, abundant OPN was detected in BMCs from overt MM patients, whereas no OPN was detected in BMCs from patients with other haematological diseases, including MGUS. Cultured BMCs from overt MM patients produced more OPN than those from patients with either smouldering MM or MGUS. Myeloma cell lines spontaneously produced OPN. Plasma OPN levels of MM patients were significantly higher than those of MGUS patients and healthy volunteers (Pu2003<u20030·05). Moreover, they correlated with both progression and bone destruction of the disease (Pu2003<u20030·05). These suggest that myeloma cells actively produce OPN, which possibly contributes to osteoclastic bone resorption in MM. Plasma OPN levels may be a useful biomarker for assessing bone destruction in MM and distinguishing MM from MGUS or smouldering MM.

Infiltrating eosinophils and eotaxin: their association with idiopathic eosinophilic esophagitis

BACKGROUNDnIdiopathic eosinophilic esophagitis (IEE) is a very rare disease characterized by thickening and eosinophil infiltration of the esophagus. The most potent chemotactic factor for eosinophils is eotaxin, and its pathophysiologic significance in IEE needs to be elucidated.nnnOBJECTIVEnTo study the association between eotaxin and IEE.nnnMETHODSnWe examined eotaxin expression in the esophagus of an IEE patient in comparison to controls by immunohistochemistry using a monoclonal antibody for human eotaxin. We also measured the free eotaxin level and the total (free and bound-form) eotaxin level in blood by enzyme-linked immunosorbent assay before and after the initiation of steroid therapy.nnnRESULTSnMost of the infiltrating eosinophils in the affected esophageal tissue showed immunohistochemical staining with anti-eotaxin antibody. In blood samples, the free eotaxin level was slightly elevated before treatment, whereas the total eotaxin level was within the normal range. Unexpectedly, the total eotaxin level increased dramatically after the initiation of steroid therapy, whereas the increase of free eotaxin was modest.nnnCONCLUSIONnInfiltrating eosinophils that express eotaxin and the changes of blood eotaxin levels during steroid therapy suggest that eotaxin may be associated with IEE.

Encapsulation of titanium(IV) oxide particles in hollow silica for size-selective photocatalytic reactions

A core-shell composite of TiO2 particles encapsulated in a hollow silica was fabricated, and the core-shell composite showed size-selective photocatalytic activity for decomposition of organics without reducing the intrinsic activity of the naked TiO2 core.

A case of multicentric Castleman's disease demonstrating severe eosinophilia and enhanced production of interleukin-5.

Abstract: Introduction: Castlemans disease (CD), idiopathic lymph‐node hyperplasia, is a heterogeneous disease of unknown origin. Although the pathophysiology is yet to be elucidated, interleukin (IL)‐6 produced by swollen lymph nodes has been reported to play a crucial role in CD.

Functions of a Co-Transmitter, Calcitonin Gene-Related Peptide, on the Neuromuscular Junction

Calcitonin gene-related peptide (CGRP) is a peptide that is synthesized in nervous tissues by a different processing of the messenger RNA for calcitonin (Amara et al.,1982; Rosenfeld et al.,1983) . A wide, but non-random distribution of CGRP-like immunoreactive structures in the central and peripheral nervous system has been demonstrated by immunohistochemistry and radioimmunoassay (Rosenfeld et al.,1983; Gibson et al.,1984; Kawai et al.,1985). CGRP has been shown to cause physiological responses and to have specific binding sites, suggesting that it is a neurotransmitter or neuromodulator.

A STUDY ON DEVELOPMENT OF VLBI CORRELATION SUBSYSTEM TRIAL PRODUCT

We present the performance test results of VLBI Correlation Subsystem (VCS) trial product which was being developed for 1 year from August 2007. It is a core component of Korea-Japan Joint VLBI Correlator (KJJVC). The aim for developing VCS trial product is to improve the performance of VCS main product to reduce the efforts and cost, and to solve the design problems by performing the preliminary test of the manufactured trial product. The function of VCS trial product is that it is able to process the 2 stations-1 baseline, 8 Gbps/station speed, 1.2 Gbps output speed with FX-type. VCS trial product consists of Read Data Control Board (RDC), Fourier Transform Board (FTB), and Correlation and Accumulation Board (CAB). Almost main functions are integrated in the FTB and CAB board. In order to confirm the performance of VCS trial product functions, the spectral analysis, delay compensation and correlation processing experiments were carried out by using simulation and real observation data. We found that the overflow problem of re-quantization after FFT processing was occurred in the delay compensation experiment. We confirmed that this problem was caused by valid bit-expression of the re-quantized data. To solve this problem, the novel method will be applied to VCS main product. The effectiveness of VCS trial product has been verified through the preliminary experimental results, but the overflow problem was occurred.

VLBI astrometry toward Sgr D HII region with VERA

The Galactic centre region shows outstanding non-circular motion unlike the Galactic disk. As scenarios describing this non-circular motion, resonance orbits formed by the Galactic bar potential or expanding motion by past activity of the central BH are proposed. However, these both scenarios are based on line-of-sight velocities of molecular clouds in this region, and such one-dimension velocity information is insufficient to separate these scenarios. To reveal dynamics of the Galactic centre region, we conducted astromertic observations of 22 GHz water maser sources toward the Galactic centre direction. We conducted astrometric observations toward water maser source associated with Sgr D HII region. As a result, we succeeded to measure the parallax and proper motion of the maser source. The measured distance was 2.36(+0.58/-0.39) kpc. This result clearly indicates that this source is not associated with the Galactic centre, but located on the Galactic disk.

RADIO ASTROMETRIC OBSERVATIONS AND THE GALACTIC CONSTANT AS THE BASIS OF A GALACTIC KINEMATICS STUDY

We made phase-referencing Very Long Baseline Interferometry (VLBI) observations of Galactic 22 GHz H2O maser sources with VLBI Exploration of Radio Astrometry (VERA). We measured the parallax dis-tances of G48.61+0.02, G48.99-0.30, G49.19-0.34, ON1, IRAS 20056+3350, IRAS 20143+3634, ON2N, and IRAS 20126+4104, which are located near the tangent point and the Solar circle. The angular ve-locity of the Galactic rotation at the LSR (i.e. the ratio of the Galactic constants) is derived using the measured parallax distances and proper motions of these sources. The derived value of nΩ 0 = 28.8 ± 1.7 km s -1 kpc -1 is consistent with recent values obtained using VLBI astrometry but 10% larger than the International Astronomical Union (IAU) recommended value of 25.9 km s -1 kpc -1 = (220 km s -1 ) / (8.5 kpc).