Hirofumi Terai

Anti-tumor polysaccharide from the mycelium of liquid-cultured Agaricus blazei mill

Anti‐tumor active polysaccharide against Sarcoma 180 was isolated by DEAE‐Sepharose CL‐6B and Sepharose 4B column chromatography from the hot‐water soluble fraction of the mycelium of liquid‐cultured Agaricus blazei mill. This polysaccharide did not react with antibodies of anti‐tumor polysaccharides such as lentinan, gliforan, and FIII‐2‐b which is one of anti‐tumor polysaccharides from Agaricus blazei. Moreover, the analyses of 13C‐NMR and GC‐MS suggested that this polysaccharide was preliminarily glucomannan with a main chain of β‐1,2‐linked D‐mannopyranosyl residues and β‐D‐glucopyranosyl‐3‐O‐β‐D‐glucopyranosyl residues as a side chain. This polysaccharide was completely different from the anti‐tumor polysaccharide from fruiting body of Agaricus blazei, β‐1,6‐glucan.