Hiromi Ike

Injury of Microvasculization on Differential Positions in the Rats Tongue Dorsum after Nd: YAG Constant power Laser Irradiation

The purpose of this study was to examine the depth into penetration of the tissue and the circulatory structure of the microvasculature after irradiation the Nd: YAG laser on the lingual dorsum of a 9-week-old male wistar rat. The Nd: YAG laser was irradiated against differential position on the lingual dorsum of a rat under the 3Watt for 1.0 second.Wounds of tongue dorsum were observed macroscopically, microscopically and also examined by SEM.The following results were obtained;Microscopical findings: The penetration of left tongue dorsum anterior group was observed still the musculi longitudinalis superior. The penetration of right margo linguae group was observed still the musculi longitudinalis superior. The penetration of tongue dorsum posterior group was observed still the musculi tranversus linguae superior.SEM findings: left tongue dorsum anterior group disappeared to the circulatory structure of the microvasculature. Right margo linguae group disappeared to the circulatory structure of the microvas culature. Right tongue dorsum posterior group disappeared to the lamina propria arteriolar and venular networks, arterioles and venulea were exposed.Irradiation power of the Nd: YAG laser need to change by differential position on the lingual dorsum of the rat.

2313 Age-related changes of tanycytes in the median eminence of the male rat

SHUJI HIDAKA, EM1 ITATEYAMA, SEIYA KONDO, SEIICHI CHIBA, TETSUYA KAKUYA, MAMORU KUROKAWA, HIRONOBU YOSHIMATSU, TOSHIIE SAKATA Since the cloning of the obese (ob) gene and ob receptor (diabetes, db) genes, evidence has been accumulating that the ob system is a key regulatory pathway associated with mammalian body weight homeostasis. We investigated that hypothalamic histamine content of the Zucker fa/fa rat and db/db mouse, in both of which abnormalities of leptin receptor had been detected, was significantly lower than that in lean littermate. Intracerebroventricular (ICV) administration of 1 pg leptin increased hypothalamic histamine turnover rate in male Wister King A rat. The pretreatment of a-fluoromethylhistidine, a specific suicide inhibitor of a histamine synthesizing decarboxylase enzyme (HDC), attenuated the anorexic effects of leptin ICV administration. In conclusion, our present observations suggest that hypothalamic histamine is one of the important target of leptin action in the brain.