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Featured researches published by Hiron M. Harshan.


Animal Reproduction Science | 2009

Identification of PDC-109-like protein(s) in buffalo seminal plasma

Hiron M. Harshan; Surya Sankar; L.P. Singh; Manish Kumar Singh; S. Sudharani; M.R. Ansari; S.K. Singh; A.C. Majumdar; Paritosh Joshi

The FN-2 family of seminal plasma proteins represents the major protein fraction of bovine seminal plasma. These proteins also constitute the major seminal plasma proteins fraction in horse, goat and bison seminal plasma and are present in pig, rat, mouse, hamster and human seminal plasma. BSP-A1 and BSP-A2, the predominant proteins of the FN-2 family, are collectively termed as PDC-109. Fn-2 proteins play an important role in fertilization, including sperm capacitation and formation of oviductal sperm reservoirs. Significantly, BSP proteins were also shown to have negative effects in the context of sperm storage. No conclusive evidence for the presence of buffalo seminal plasma protein(s) similar to PDC-109 exists. Studies with buffalo seminal plasma indicated that isolation and identification of PDC-109-like protein(s) from buffalo seminal plasma by conventional methods might be difficult. Thus, antibodies raised against PDC-109 isolated, and purified from cattle seminal plasma, were used for investigating the presence of PDC-109-like protein(s) in buffalo seminal plasma. Buffalo seminal plasma proteins were resolved on SDS-PAGE, blotted to nitro cellulose membranes and probed for the presence of PDC-109-like protein(s) using the PDC-109 antisera raised in rabbits. A distinct immunoreactive band well below the 20-kDa regions indicated the presence of PDC-109-like protein(s) in buffalo seminal plasma.


Research in Veterinary Science | 2010

Evaluation of a recombinant LigB protein of Leptospira interrogans serovar Canicola in an enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis

Surya Sankar; Hiron M. Harshan; S.R. Somarajan; S. K. Srivastava

A recombinant leptospiral lipoprotein, LigB, was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLigB IgG ELISA). The standard reference test (Microscopic agglutination test, MAT) of 200 serum samples from cattle suspected of leptospirosis showed that 95 (47.5%) samples had positive agglutination titres, which ranged from 100 to 1600. In rLigB IgG ELISA, 49% of the samples were positive. Sensitivity of IgG ELISA for 95 bovine sera, which had MAT titres of greater than or equal to 100, were 100%. ELISA showed a specificity of 97.1% with 105 bovine sera, which were negative at a 1:50 dilution in MAT for Leptospira interrogans serovars. The results of ELISA and MAT correspond very good. When analytical specificity of IgG ELISA was evaluated using bovine serum samples from animals showing the serum antibodies to other pathogens, no cross-reaction was observed. Thus the recombinant LigB IgG ELISA can be used instead of the MAT as an aid to the diagnosis of bovine leptospirosis.


Animal Reproduction Science | 2006

Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa

Hiron M. Harshan; L.P. Singh; A. Arangasamy; M.R. Ansari; Satish Kumar


Animal Reproduction Science | 2007

Effect of egg yolk and seminal plasma heparin binding protein interaction on the freezability of buffalo cauda epididymal spermatozoa

L.P. Singh; Hiron M. Harshan; M.R. Ansari


Animal Reproduction Science | 2008

Seminal plasma non-heparin binding proteins (NHBP) reduce the cryoinjury to buffalo cauda epididymal spermatozoa induced by heparin binding proteins (HBP)

Anuj Kumar; L.P. Singh; Hiron M. Harshan; A.C. Majumdar


Journal of Indian Veterinary Association, Kerala | 2014

Prevalence of common haemoparasitic diseases among cattle of Thrissur district.

Surya Sankar; Sunitha Karunankaran; Hiron M. Harshan; S. Pramod; K. S. Thilakan


The Indian Journal of Veterinary Research | 2010

Evaluation of recombinant LipL32 and LipL41 antigens of Leptospira interrogans serovar Canicola by ELISA for serodiagnosis of bovine leptospirosis

Surya Sankar; Pallab Chaudhury; Rishendra Verma; Hiron M. Harshan; S. K. Srivastava


Indian Journal of Animal Research | 2009

A Comparative Study between Microscopic Agglutination Test and Different Protein Antigens Based Enzyme-Linked Immunosorbent Assay for the Detection of Leptospirosis in Bovine

Surya Sankar; Hiron M. Harshan; Pallab Chaudhary; S.K. Srivastava


Journal of Immunology and Immunopathology | 2008

Cloning and sequencing of LigB gene of Leptospira interrogans serovar Canicola

Surya Sankar; Hiron M. Harshan; E Thankapandian; Pallab Chaudhary; S. K. Srivastava


Journal of Immunology and Immunopathology | 2008

Evaluation of the comparative efficacy of recombinant protein antigens based enzyme-linked immunosorbent assay for the diagnosis of caprine leptospirosis

Surya Sankar; Hiron M. Harshan; E Thankapandian; Pallab Chaudhary; S. K. Srivastava

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Surya Sankar

Indian Veterinary Research Institute

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L.P. Singh

Indian Veterinary Research Institute

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S. K. Srivastava

Indian Veterinary Research Institute

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M.R. Ansari

Indian Veterinary Research Institute

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Pallab Chaudhary

Indian Veterinary Research Institute

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A.C. Majumdar

Indian Veterinary Research Institute

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A. Arangasamy

National Research Centre on Equines

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Anuj Kumar

Indian Veterinary Research Institute

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Manish Kumar Singh

Indian Veterinary Research Institute

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Paritosh Joshi

Indian Veterinary Research Institute

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