Hiroomi Miyazaki
Kagoshima University
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Featured researches published by Hiroomi Miyazaki.
Journal of Clinical Investigation | 1988
Eiichi Gohda; Hirohito Tsubouchi; Hiroyuki Nakayama; Shuichi Hirono; Osamu Sakiyama; Kozo Takahashi; Hiroomi Miyazaki; Shuli Hashimoto; Yasushi Daikuhara
Human hepatocyte growth factor (hHGF) has been purified approximately 209,000-fold with 18% yield from plasma of a patient with fulminant hepatic failure. The purification involves heat treatment of plasma, ammonium sulfate precipitation, and chromatography on Affi-Gel Blue, heparin-Sepharose, and hydroxylapatite. Purified hHGF shows several bands with molecular weights between 76,000 and 92,000. Each band shows growth-stimulating activity on cultured hepatocytes which is proportional to the intensity of the band. After reduction of the sample with 2-mercaptoethanol, SDS-PAGE yields two chains with molecular weights of 31,500-34,500 and 54,000-65,000. The effect of hHGF on DNA synthesis by hepatocytes is half-maximal at 3.5 ng/ml. hHGF stimulates proliferation of cultured hepatocytes more effectively than human epidermal growth factor (hEGF) or insulin, and the effect of hHGF is additive or synergistic with the maximal effects of hEGF and insulin. These results suggest that hHGF is a new growth factor which is different from hEGF.
Calcified Tissue International | 1985
Osamu Nakamura; Eiichi Gohda; Masayuki Ozawa; Ichiro Senba; Hiroomi Miyazaki; Tadashi Murakami; Yasushi Daikuhara
SummaryA monoclonal antibody was raised against phosphoryn, a unique noncollagenous phosphoprotein in dentin. Mouse myeloma NS-I cells were fused with spleen cells obtained from BALB/c mice immunized with phosphophoryn from fetal calf tooth germs. Mice inoculated with the hybridoma produced ascites fluid containing the antibody and this reacted only with a band of phosphophoryn transblotted from polyacrylamide gel. Immunohistochemical studies with the antibody showed that phosphophoryn was present in odontoblasts, odontoblastic processes and dentin, but not in the matrix of predentin, and that the phosphophoryn content of the dentin layer was high at and around the predentin-dentin junction and gradually decreased toward the enamel layer. The area corresponding to mantle dentin was not stained with the antibody.
Pharmacological Research Communications | 1987
Shuichi Hirono; Eiichi Gohda; Hirohito Tsubouchi; F. Tamada; Hiroyuki Nakayama; Kozo Takahashi; Osamu Sakiyama; Hiroomi Miyazaki; S. Baba; Yasushi Daikuhara; S. Hashimoto
The effects of three of the most widely used histamine H2-receptor antagonists, cimetidine, ranitidine and famotidine, on liver cell growth were studied in vitro using adult rat hepatocytes in primary culture, because these antagonists are commonly given to patients with hepatic cirrhosis or fulminant hepatic failure for protection against peptic ulcers and gastrointestinal hemorrhage. At their clinically effective concentrations in the blood (0.5-5 micrograms/ml cimetidine, 0.25-2.5 micrograms/ml ranitidine and 0.05-0.5 microgram/ml famotidine), these three antagonists did not have any effect on replicative DNA synthesis either in the presence or absence of insulin plus epidermal growth factor (EGF). However, unexpectedly DNA synthesis stimulated by insulin and EGF was found to be enhanced by 0.05-0.5 mg/ml cimetidine, although it was unaffected or inhibited by ranitidine and famotidine at the concentrations tested. Cimetidine caused maximal enhancement of 1.5-2 times the control level of DNA synthesis at a concentration of 0.25 mg/ml. Cimetidine also had an enhancing effect at submaximal concentrations of insulin and EGF, but neither cimetidine nor the other antagonists had any stimulatory effect on DNA synthesis in the absence of insulin plus EGF. This enhancement of DNA synthesis by cimetidine resulted in significant increase in the total DNA content of the hepatocytes in culture. Under the conditions used, cimetidine had the lowest toxicity of these three antagonists and ranitidine the highest, as judged from data on DNA synthesis and the total protein content of cultured hepatocytes, leakage of aminotransferases from the cells and morphological observations.
Hepatology | 1989
Hirohito Tsubouchi; Shuichi Hirono; Eiichi Gohda; Hiroyuki Nakayama; Kozo Takahashi; Osami Sakiyama; Hiroomi Miyazaki; Jun-ichi Sugihara; Eiichi Tomita; Yasutoshi Muto; Yasushi Daikuhara; Shuji Hashimoto
The Lancet | 1992
Hirohito Tsubouchi; Shuichi Kawakami; Shuichi Hirono; Hiroomi Miyazaki; Masayoshi Kimoto; Terukatsu Arima; Kazuhiko Sekiyama; Makoto Yoshiba; Naokatu Arakaki; Yasushi Daikuhara
Hepatology | 1995
Naokatu Arakaki; Shuichi Kawakami; Osamu Nakamura; Tomokazu Ohnishi; Hiroomi Miyazaki; Takehisa Ishii; Hirohito Tsubouchi; Yasushi Daikuhara
Biochemical and Biophysical Research Communications | 1994
Tomokazu Ohnishi; Osamu Nakamura; Naokatu Arakaki; Hiroomi Miyazaki; Yasushi Daikuhara
Internal Medicine | 2001
Shuichi Kawakami; Takushi Arima; Kouji Harada; Hirotaka Miyazono; Makoto Oketani; Hiroomi Miyazaki; Terukatsu Arima
Endocrinology | 1986
Hirohito Tsubouchi; Hiroomi Miyazaki; Eiichi Gohda; Hiroyuki Nakayama; Yoshiyuki Nakazono; Yasushi Daikuhara; Shuji Hashimoto
Endocrinologia Japonica | 1983
Hirohito Tsubouchi; Eiichi Gohda; Hiroomi Miyazaki; Atsuyuki Kamibeppu; Harumi Oka; Juen Nagahama; Shuichi Hirono; Kunio Fujisaki; Osamu Nakamura; Yasushi Daikuhara; Shuji Hashimoto