Hiroshi Aochi

Distinct effects of daratumumab on indirect and direct antiglobulin tests: a new method employing 0.01 mol/L dithiothreitol for negating the daratumumab interference with preserving K antigenicity (Osaka method): 0.01 MOL/L DTT FOR THE DARA INTERFERENCE

There is an increasing demand for daratumumab (DARA), an immunoglobulin (Ig)G1κ monoclonal antibody (MoAb) that recognizes CD38, to manage relapsed or refractory multiple myeloma (MM) patients. However, DARA leads to positive and panreactive agglutination reactions in indirect antiglobulin tests (IATs) in vitro (the DARA interference). In addition, effects of DARA on red blood cells (RBCs) in vivo remains elusive.

Report on ABO-Incompatible Transfusions in 12 University Hospitals in Kinki Districts.

We investigated the incidence and the causes of ABO-incompatible transfusion in 12 university hospitals in the Kinki district during the 5-year period from 1993 through 1997. Over 5 years, 648, 553 units of red cell components (RBC) and 2, 545, 880 units of fresh frozen plasma (FFP) or platelet concentrate (PC) were transfused, and a total of 26 ABO-incompatible transfusions occurred. Incompatible RBC were transfused to 17 patients and FFP or PC were transfused to the remaining 9 patients. In cases of RBC transfusion, half of errors (8 cases) were due to misidentification of blood units and the other half of errors (8 cases) were due to incorrect typing. Twenty of 26 errors occurred during night shifts and the majority of errors occurred in the ward (17 cases) or operating room (5 cases). Various measures were taken to prevent ABO-incompatible transfusion at the participating universities. We conclude that continued data collection and analysis are necessary to prevent ABO-incompatible transfusion.

A case of immune hemolytic anemia due to "auto" anti-A1 antibody induced by group O homograft in renal transplantation recipient.

A case of severe auto-immune hemolytic anemia after a living, ABO-incompatible renal transplantation is reported.The recipient, a 34 years old man, blood group A1, Rh0 (D) positive receieved a renal transplant from father whose blood group O, Rh0(D) positive donor. Cyclosporine, prednisolone and mizoribine were used for immunosuppresion.The recipient devloped severe immune hemolytic anemia within 2 weeks of renal transplantation. The recipients serum contained anti-A1 IgG autoantibody and anti-B IgG antibody on day 18 post-transplantation. The IgG subclass of these antibodies was IgG3.

ELISA for detection of red cell antibodies using red cell ghosts coated plates. The detection of Rh and other antibodies.

An ELISA using micro-plates coated with red cell ghosts was developed for analysis of red cell antibodies.Antibodies against MNSs, Kell, Duffy and Kidd antigens were detected using this ELISA. Rh antibodies in patients sera could be also detected by this assay, but the elution technique was needed when the titer of antibody was low.We considered that this assay was quantitative, objective and sensitive compared with indirect antiglobulin test, and easy to perform compared with ELISA of tube method using red cell suspensions.