Hiroshi Kusano

Sepabeads FP series: New packing materials for industrial-scale separation of biopolymers

SummarySepabeads FP series with a variety of anion-exhanger type functional groups have been examined as largescale chromatographic separation media for proteins. The adsorption-desorption behavior of proteins has been found to be dependent mainly on the type and partly on the content of the attached amino ligands. The effect of the attached groups are discussed using batch measurement data. It has been found that the existence of ethanol residue on the nitrogen atom incrases the hydrophilic nature of quaternary ammonium type packings and thus gives a higher protein adsorption capacity. Among weakly basic type anionexchangers, polyethyleneimine-modified packings showed very large protein capacities.

Sepabeads® FP series: new highly porous hydrophilic supports for protein separation

Abstract The use of the new chromatographic packing material, Sepabeads® FP series , has been examined in industrial-scale separations. They showed sufficient stability and have a highly porous hydrophilic nature which makes them suitable for the purification of biopolymers such as proteins. It was found that the protein adsorption capacity strongly depends on the porosity, i.e., mean pore radius, of the packing as well as on the type of attached functional groups. Using Sepabeads FP series, industrial-scale separations of biopolymers in various chromatographic modes have become practical. Basic properties of Sepabeads FP series and some applications to the separation of proteins are briefly described.