Hiroshi Shimasue

A Case of SAPHO Syndrome With Diffuse Sclerosing Osteomyelitis of the Mandible Treated Successfully With Prednisolone and Bisphosphonate

*Department of Oral and Maxillofacial Surgery, Division of Cervico Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. †Department of Oral and Maxillofacial Surgery, Division of Cervico Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. ‡Department of Oral and Maxillofacial Surgery, Division of Cervico Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. §Department of Oral and Maxillofacial Surgery, Division of Cervico Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. Department of Oral and Maxillofacial Surgery, Division of Cervico Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. ¶Department of Clinical Immunology and Rheumatology, Hiroshima University Hospital, Hiroshima, Japan. #Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. **Department of Oral and Maxillofacial Surgery, Division of Cervico Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. ††Department of Surgery, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. ‡‡Department of Oral and Maxillofacial Surgery, Division of Cervico Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. Address correspondence and reprint requests to Dr Shigeishi: Department of Oral and Maxillofacial Surgery, Division of CervicoGnathostomatology, Graduate School of Biomedical Sciences, Hirod

Snail promotes Cyr61 secretion to prime collective cell migration and form invasive tumor nests in squamous cell carcinoma

We previously identified genes associated with Snail-mediated squamous cell carcinoma (SCC) invasiveness, in which we observed significant elevation of Cyr61 expression. In this study, SCC cell lines overexpressing Cyr61 exhibited constitutive activation of Rho A and upregulated invasiveness without the disruption of homophilic cell attachment. Humoral Cyr61 enhanced further production of endogenous Cyr61 by SCC cells, which stimulated collective cell migration and the development of an invasive tumor nest. We propose a Cyr61-dependent model for the development of invasive SCC nest, whereby a subset of tumor cells that highly produce Cyr61 may direct other tumor cells to undergo collective cell migration, resulting in a formation of primary SCC mass.

Autocrine galectin-1 promotes collective cell migration of squamous cell carcinoma cells through up-regulation of distinct integrins.

We found that high galectin-1 (Gal-1) mRNA levels were associated with invasive squamous cell carcinoma (SCC) cells that expressed Snail, an epithelial-to-mesenchymal transition (EMT) regulator. Both Gal-1 overexpression and soluble Gal-1 treatment accelerated invasion and collective cell migration, along with activation of cdc42 and Rac. Soluble Gal-1 activated c-Jun N-terminal kinase to increase expression levels of integrins α2 and β5, which were essential for Gal-1 dependent collective cell migration and invasiveness. Soluble Gal-1 also increased the incidence of EMT in Snail-expressing SCC cells; these were a minor population with an EMT phenotype under growing conditions. Our findings indicate that soluble Gal-1 promotes invasiveness through enhancing collective cell migration and increasing the incidence of EMT.

Establishment of mesenchymal cell line derived from human developing odontoma.

OBJECTIVES An odontoma, which shows proliferating odontogenic epithelium and mesenchymal tissue, is one of the most common odontogenic tumors encountered. These are commonly found in tooth-bearing regions, although the etiology remains unknown. There are no previous reports of an established line of immortalized human odontoma cells. METHODS Using odontoma fragments obtained from a girl treated at our department, we established an immortalized human odontoma cell line and investigated cell morphology, dynamic proliferation, the presence of contamination, and karyotype. Moreover, cell characterization was examined using osteogenic and odontogenic markers. RESULTS We successfully established a mesenchymal odontoma cell (mOd cells). The cells were found to be fibroblastic and had a high level of telomerase activity. Cell growth was confirmed after more than 200 population doublings without significant growth retardation. mOd cells expressed mRNA for differentiation markers, including collagen type I (COLI), alkaline phosphatase, bone sialoprotein, osteopontin, osteocalcin, cementum-derived protein (CP-23), dentin sialophosphoprotein (DSPP), and distal-less homeobox 3 (DLX3), as well as bone morphogenetic proteins (BMPs). In addition, they showed a high level of calcified nodule formation activity in vitro. CONCLUSIONS We successfully established a cell line that may be useful for investigating the mechanisms of normal odontogenesis as well as characteristics of odontoma tumors.

Cat Scratch Disease

Abstract Cat scratch disease presenting as an enlarged submental lymph node in a 5-year-old boy is reported. There was clear history of contact with a cat, raised immunoglobulin G antibody titre against Bartonella henselae , and a granulomatous reaction in the excised lymph node.