Hiroshi Takamatsu

Condensation heat transfer of binary refrigerant mixtures of R22 and R114 inside a horizontal tube with internal spiral grooves

Abstract An experimental study of the condensation of pure and mixed refrigerants of R22 and R114 inside a spirally grooved horizontal copper tube has been carried out. A double-tube counterflow condenser in the pressure range 3–21 bar and at a mass flow-rate 26–70 kg h −1 was used. The axial distributions of refrigerant, tube wall and cooling water temperatures, wall heat flux density and vapour quality are shown graphically. The variation of tube wall temperature around the circumference of the tube is also shown. The local Nusselt number depends on the molar fraction, whereas the average Nusselt number can be correlated by an equation which is modified from a previously established equation for pure refrigerants inside a horizontal smooth tube. The frictional pressure drop evaluated is correlated well by the Lockhart-Martinelli parameters and is independent of the concentration of the mixture.

A correlation for forced convective boiling heat transfer of pure refrigerants in a horizontal smooth tube

Abstract An experimental study is reported on the boiling heat transfer of HFC134a, HCFC22, CFC114 and CFC12 flowing inside a 7.9 mm ID horizontal smooth tube. Using a water-heated, double-tube type evaporator, the local heat transfer coefficients are measured for both counter and parallel flows. Based on the supposition of Chen that the total heat flux is represented as the sum of forced convective contribution and nucleate boiling contribution, a correlation equation is proposed for the data in the annular-flow regime. The mean deviation between the calculated and measured heat transfer coefficients is 12.2% for the present experimental data and 9.5% for the data available from literature. The proposed correlation shows that the nucleate boiling is not fully suppressed even in the high-quality region in the case of counter flow, while convective evaporation is dominant in the high-quality region with uniform heat flux condition.

Quantitative Examination of a Perfusion Microscope for the Study of Osmotic Response of Cells

The perfusion microscope was developed for the study of the osmotic response of cells. In this microscope, the cells are immobilized in a transparent chamber mounted on the stage and exposed to a variety of milieus by perfusing the chamber with solutions of different concentrations. The concentration of the supplied solution is controlled using two variable-speed syringe pumps, which supply an isotonic solution and a hypertonic solution. Before using this system to characterize the osmotic response of cells, the change in the concentration of NaCl solution flowing through the chamber is examined quantitatively using a laser interferometer and an image processing technique. The NaCl concentration is increased from an isotonic condition to a hypertonic condition abruptly or gradually at a given constant rate, and decreased from a hypertonic condition to an isotonic condition. It is confirmed that the concentration is nearly uniform in the cross direction at the middle of the chamber, and the change in the NaCl concentration is reproducible. The average rate of increase or decrease in the measured concentration agrees fairly well with the given rate when the concentration is changed gradually at a constant rate. The rate of the abrupt change is also determined to be the highest limit achieved by the present method. As the first application of using the perfusion microscope for biological studies, the volume change of cells after exposure to a hypertonic solution is measured. Then, the hydraulic conductivity of the cell membrane is determinedfrom the comparison of the volume change between the experiment and the theoretical estimation for the measured change in the NaCl concentration of the perfused solution.

Condensation of downward-flowing HFC134a in a staggered bundle of horizontal finned tubes: effect of fin geometry

Experimental results are presented that show the effect of fin geometry on condensation of refrigerant HFC134a in a staggered bundle of horizontal finned tubes. Two types of conventional low-fin tubes and three types of three-dimensional fin tubes were tested. The refrigerant mass velocity ranged from 8 to 23 kg/m2s and the condensation temperature difference from 1.5 to 12 K. The effect of condensate inundation was more significant for the three-dimensional fin tubes than for the low-fin tubes. In most cases, the highest performance was obtained by the tube with a three-dimensional structure at the tip of low fins. In the case of high mass velocity and high condensate inundation rate, however, the highest performance was obtained by one of the low-fin tubes. The results were compared with previous results for bundles of smooth tubes and low-fin tubes.

Experimental study of thermal rectification in suspended monolayer graphene

Thermal rectification is a fundamental phenomenon for active heat flow control. Significant thermal rectification is expected to exist in the asymmetric nanostructures, such as nanowires and thin films. As a one-atom-thick membrane, graphene has attracted much attention for realizing thermal rectification as shown by many molecular dynamics simulations. Here, we experimentally demonstrate thermal rectification in various asymmetric monolayer graphene nanostructures. A large thermal rectification factor of 26% is achieved in a defect-engineered monolayer graphene with nanopores on one side. A thermal rectification factor of 10% is achieved in a pristine monolayer graphene with nanoparticles deposited on one side or with a tapered width. The results indicate that the monolayer graphene has great potential to be used for designing high-performance thermal rectifiers for heat flow control and energy harvesting.

In situ harvesting of adhered target cells using thermoresponsive substrate under a microscope: principle and instrumentation.

A novel technique and instrumented device were developed to harvest target cells from multicellular mixture of different cell types under a microscope. The principle of the technique is that cells cultured on a thermoresponsive-substance-coated dish were detached by a region-specific cooling device and simultaneously harvested using a micropipette, both of which were assembled in an inverted microscope. Thermoresponsive coating consists of the mixture of poly(N-isopropylacrylamide) (PNIPAAm) and PNIPAAm-grafted gelatin. The former non-cell-adhesive polymer dissolves below at 32.1 degrees C in water and precipitates over that temperature (called lower critical solution temperature, LCST), and the latter cell-adhesive polymer has LCST of 34.1 degrees C. The appropriate mixing ratio of these thermoresponsive polymers exhibited high cell adhesion at physiological temperature and complete cell detachment at room temperature. A device developed as to cool at only a tiny area of the bottom of the dish, beneath which a cell that was targeted under a microscope, was assembled in a microscope. It was demonstrated that single cell or two cells that adhered to each other was detached from the surface and harvested by a micropipette within approximately 30s.

Experimental measurements for condensation of downward-flowing R123/R134a in a staggered bundle of horizontal low-finned tubes with four fin geometries

Experiments were conducted to obtain row-by-row heat and mass transfer data during condensation of downward-flowing zeotropic mixture R123/R134a in a staggered bundle of horizontal low-finned tubes. The vapor temperature and the mass fraction of R134a at the tube bundle inlet were about 50°C and 14%, respectively. The refrigerant mass velocity ranged from 9 to 34 kg m−2 s−1, and the condensation temperature difference from 1.9 to 12 K. Four kinds of low-finned tubes with different fin geometry were tested. The highest heat transfer coefficient was obtained with a tube which showed the highest performance for R123. However, the diference among the tubes was much smaller for the mixture than for R123. The heat transfer coefficient and the vapor-phase mass transfer coefficient decreased significantly with decreasing mass velocity. The mass transfer coefficient increased with condensation temperature difference, which was due to the effect of suction associated with condensation. On the basis of the analogy between heat and mass transfer, a dimensionless correlation of the mass transfer coefficient was developed for each tube.

In Situ Spectroscopic Quantification of Protein-Ice Interactions

FTIR and confocal Raman microspectroscopy were used to measure interactions between albumin and ice in situ during quasi-equilibrium freezing in dimethyl sulfoxide (DMSO) solutions. At temperatures of -4 and -6 °C, albumin was found to be preferentially excluded from the ice phase during near-equilibrium freezing. This behavior reversed at lower temperatures. Instead, DMSO was preferentially excluded from the ice phase, resulting in an albumin concentration in the freeze-concentrated liquid phase that was lower than predicted. It is hypothesized that this was caused by the albumin in the freeze-concentrated liquid getting adsorbed onto the ice surface or becoming entrapped in the ice phase. It was observed that, under certain freezing protocols, as much as 20% of the albumin in solutions with starting concentrations of 32-53 mg/mL may be adsorbed onto the ice interface or entrapped in the ice phase.

Vapor absorption by LiBr aqueous solution in vertical smooth tubes

Abstract Heat and mass transfer in a falling film vertical in-tube absorber was studied experimentally with LiBr aqueous solution. The presented results include the effect of solution flow rate, solution subcooling and cooling water temperature on the absorption in a smooth copper tube 16.05 mm I.D. and 400 mm long. The experimental data in the previous report for a 1200-mm-long tube was also re-examined and compared. It was demonstrated by the observation of the flow in the tube that the break down of the liquid film into rivulets leads to deterioration of heat and mass transfer at lower film Reynolds number or in longer tubes. An attempt to evaluate physically acceptable heat and mass transfer coefficients that are defined with estimated temperature and concentration at the vapor–liquid interface was also presented.

Survival of biological cells deformed in a narrow gap

Recent studies show that during slow freezing of biological cells, the cells may be also injured by not only chemical damage but also mechanical damage induced by ice crystal compression. A new experimental procedure is developed to quantify cell destruction by deformation with two parallel surfaces. The viability of cells (prostatic carcinoma cells, 17.5 microns in mean diameter) is measured as a function of gap size ranging from 3.5 microns to 16.2 microns at 0 degree C, 23 degrees C and 37 degrees C. The viability at a smaller gap size is significantly lower at 37 degrees C than at 23 degrees C, while the difference between 0 degree C and 23 degrees C is much smaller. This suggests that deformation damage is related to the deformation of the cytoskeleton rather than the mechanical properties of the lipid membrane.