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Dive into the research topics where Hiroshige Itakura is active.

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Featured researches published by Hiroshige Itakura.


Atherosclerosis | 1988

A study of DNA polymorphism in the apolipoprotein B gene in a Japanese population.

Hiroyuki Aburatani; Akiyo Matsumoto; Hiroshi Itoh; Nobuhiro Yamada; Toshio Murase; Fumimaro Takaku; Hiroshige Itakura

A Japanese group comprising 53 hyperlipidemic and 54 normolipidemic subjects was genotyped for DNA restriction fragment length polymorphisms (RFLPs) at the apo B gene locus. The polymorphisms with XbaI and PvuII were present at allelic frequencies of 0.04 (X1 allele) and 0.96 (X2 allele), 0.94 (P1 allele) and 0.06 (P2 allele), respectively. Unlike the previous reported association of the X1 allele with hypercholesterolemia found in Caucasians there was no difference in the frequency of the X1 allele between normolipidemic and hypercholesterolemic Japanese. Among the Japanese, two RFLPs appear to be in linkage equilibrium and can be used in conjunction as a haplotype. There is no strong population association in our patient group between any allele of the RFLPs studied and hyperlipidemia.


Diabetes | 1992

Exercise Training Increases Glucose Transporter Content in Skeletal Muscles More Efficiently From Aged Obese Rats Than Young Lean Rats

Osamu Ezaki; Mitsuru Higuchi; Haruko Nakatsuka; Kentaro Kawanaka; Hiroshige Itakura

Glucose uptake in rat skeletal muscles decreases with age and obesity, but increases with chronic exercise training. The purpose of our study was to determine whether the GLUT4 content in several skeletal muscles from 1-mo-old young, lean rats and 12-mo-old aged, obese rats alters with exercise training. For exercise, a treadmill run of ∼ 1 km/day was made for 4 wk by both groups of rats. The concentration of GLUT4 per protein in membrane fraction from several skeletal muscles was measured by immunoblotting. The amount of GLUT4 in the gastrocnemius and white quadriceps from aged rats slightly but significantly decreased to 73% and 78% of that from young rats, respectively. However, no significant difference in GLUT4 amount in the soleus, plantaris, and red quadriceps was observed between young and aged rats. The exercise training resulted in a larger increase in the amount of GLUT4 in each muscle from aged rats than in muscles from young rats. In aged rats, GLUT4 amount increased significantly with exercise training by 30, 33, 41, and 27% in the soleus, plantaris, gastrocnemius, and red quadriceps, respectively, compared with the sedentary controls. However, in young rats, exercise-induced increase of GLUT4 amount was significant only in the plantaris, and the increase was 17%. In exercised aged, obese rats, decreases of body weight, plasma triglyceride levels, and plasma free fatty acid were also observed. These findings suggest that the amount of GLUT4 in some skeletal muscles decreases slightly in aged, obese rats, exercise training increases the amount of GLUT4 more efficiently in aged rats than in young rats and ameliorates the GLUT4 decrease observed in aged rats, and lipid metabolism may be related to the regulation of GLUT4 protein content in muscles.


Biochemical and Biophysical Research Communications | 1987

Cloning and regulation of rat apolipoprotein B mRNA

Akiyo Matsumoto; Hiroyuki Aburatani; Yoshikazu Shibasaki; Tatsuhiko Kodama; Fumimaro Takaku; Hiroshige Itakura

Recombinant cDNA clones that code for apolipoprotein B(apoB) were isolated from a rat liver cDNA library, using synthetic oligonucleotide probe derived from the sequence of human apoB cDNA. The nucleotide and deduced amino acid sequences of the rat apoB clone pRB5, 1.2 kb in length, showed 83% and 84% homology to those of human apoB. Northern blot analysis revealed that rat apoB cDNA probe cross-reacts with human and rabbit apoB mRNA sequences and the size of those mRNAs, approximately 15 kb long, were not discernibly different. In addition, apoB mRNA was abundant only in the liver and intestine. Finally, cholesterol feeding to rats for six weeks resulted in a several-fold increase in the level of apoB mRNA in the liver.


Biochemical and Biophysical Research Communications | 1989

Co-localization of a glucose transporter and the insulin receptor in microsomes of insulin-treated rat adipocytes.

Osamu Ezaki; Naoko Bono; Hiroshige Itakura; Michihiro Kasahara

Microsomal vesicles prepared from rat adipocytes were immuno-adsorbed to formaldehyde-fixed Staphylococcus aureus cells (Pansorbin) coated with anti-human-erythrocyte-glucose-transporter IgG. More than 75% of the glucose transporter detected was precipitated. The glucose transporter was about 10-fold enriched by the adsorption procedure. On insulin treatment, the insulin receptor in plasma membranes was internalized and the receptor in the microsome fraction increased 5-fold. Thirty-five % of the insulin receptor in the microsome fraction was recovered with the glucose-transporter-containing vesicles. These observations indicate that on insulin treatment a considerable portion of the microsome vesicles containing the insulin receptor fuses or becomes tightly associated with ones containing the glucose transporter.


American Journal of Physiology-endocrinology and Metabolism | 1997

Cholate inhibits high-fat diet-induced hyperglycemia and obesity with acyl-CoA synthetase mRNA decrease

Shinji Ikemoto; Mayumi Takahashi; Nobuyo Tsunoda; Kayo Maruyama; Hiroshige Itakura; Kentaro Kawanaka; Izumi Tabata; Mitsuru Higuchi; Tsuyoshi Tange; Tokuo T. Yamamoto; Osamu Ezaki


Journal of Nutritional Science and Vitaminology | 2003

Analysis of the Comprehensive Effects of Polyunsaturated Fatty Acid on mRNA Expression Using a Gene Chip

Yoko Fujiwara; Masayo Yokoyama; Rumi Sawada; Yousuke Seyama; Masami Ishii; Shunichi Tsutsumi; Hiroyuki Aburatani; Satoko Hanaka; Hiroshige Itakura; Akiyo Matsumoto


Japanese Journal of Medicine | 1988

Deoxyribonucleic Acid (DNA) Polymorphism in the Apolipoprotein AI Gene: A Study in a Japanese Population

Hiroyuki Aburatani; Akiyo Matsumoto; Hiroshi Itoh; Toshio Murase; Fumimaro Takaku; Hiroshige Itakura


Biochemical and Biophysical Research Communications | 1994

Stabilization of Interleukin-2 Receptor-α Chain mRNA by HTLV-1 Rex in Mouse L Cells: Lower Amounts of Rex Do Not Stabilize the mRNA

Hiroshi Kanamori; Tatsuhiko Kodama; Akiyo Matsumoto; Hiroshige Itakura; Yoshio Yazaki


Journal of Atherosclerosis and Thrombosis | 2002

Influence of polyunsaturated fatty acid on gene expression in HepG2 cells.

Yoko Fujiwara; Masayo Yokoyama; Rumi Umeda-Sawada; Yousuke Seyama; Masami Ishii; Shunichi Tsutsumi; Hiroyuki Aburatani; Satoko Hanaka; Hiroshige Itakura; Akiyo Matsumoto


Atherosclerosis | 2000

Exploring new functions of polyunsaturated fatty acids on the gene expression using DNA chip

Akiyo Matsumoto; Yoko Fujiwara; S. Hanaka; Hiroshige Itakura; Masami Ishii; Shunichi Tsutsumi; Hiroyuki Aburatani

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Kentaro Kawanaka

Niigata University of Health and Welfare

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