Hiroto Shima

Inhibitory effect of chlorogenic acid on methylazoxymethanol acetate-induced carcinogenesis in large intestine and liver of hamsters

The effect of dietary chlorogenic acid on methylazoxymethanol (MAM) acetate-induced carcinogenesis was examined in Syrian golden hamsters. The combined incidence of total large intestinal tumors from male and female hamsters, and the combined incidence of large intestinal adenocarcinomas or the incidence of the carcinomas of male or female animals of the group given a single intravenous injection of MAM acetate (20 mg/kg body wt) and then fed the diet containing 0.025% chlorogenic acid for 24 weeks were significantly lower than those of hamsters given MAM acetate alone. The numbers of hyperplastic liver cell foci in male and female hamsters given MAM acetate and chlorogenic acid were also significantly smaller than those of hamsters given MAM acetate alone. These results indicate an inhibitory effect of chlorogenic acid on MAM acetate-induced carcinogenesis in hamsters.

Inhibitory effects of chlorogenic acid, reserpine, polyprenoic acid (E-5166), or coffee on hepatocarcinogenesis in rats and hamsters.

Four different experiments were performed in order to examine the modifying effects of chlorogenic acid (CA), reserpine, polyprenoic acid (E-5166), and coffee on chemical carcinogenesis in rats or hamsters. Experiment 1: The numbers of hyperplastic liver cell foci and the incidence of colon tumors in male and female Syrian golden hamsters given a single intravenous injection of methylazoxymethanol (MAM) acetate and then fed the diet containing 0.025% CA for 24 wk were significantly lower than those of hamsters given MAM acetate alone. Experiment 2: The incidence of altered hepatocellular foci in female ACI/N rats given N-2-fluorenylacetamide (FAA, 0.02% in diet) for 10 wk and reserpine (weekly subcutaneous injections, 1 microgram/g body weight) during or after (17 wk) FAA exposure was significantly lower than that of rats given FAA alone. Experiment 3: The number of hepatocellular foci in male ACI/N rats given 0.02% FAA diet for 13 wk and E-5166 by gavage (40 mg/kg body weight, 3 times/wk) for 16 wk after the end of FAA exposure was significantly smaller than that in rats given FAA diet alone. Experiment 4: Incidences of liver tumors and hepatocellular foci of rats given concurrent dietary administration of aminopyrine (0.01%) and sodium nitrite (0.1%) and coffee solution as a drinking water for 630 da were significantly lower than those of rats given aminopyrine and sodium nitrite. Thus, the tested compounds had inhibitory effects on chemical carcinogenesis in liver or colon.

A case of renal cell carcinoma solitarily metastasized to thyroid 20 years after the resection of primary tumor.

A case of renal cell carcinoma in a 69-year-old woman is reported. The woman developed a thyroid tumor recently. She has a history of nephrectomy done 20 years ago for a renal cell carcinoma. Based on the close resemblances of the histological features and cell nuclear DNA distributions of both tumors, the thyroid tumor was diagnosed as the secondary tumor from the renal cell carcinoma. In the review of the literature, this case of renal cell carcinoma seems to be that with the longest interval of years before solitary metastasis to the thyroid.

Esophageal metastasis from prostate cancer: Diagnostic use of reverse transcriptase-polymerase chain reaction for prostate-specific antigen

An extremely rare case of esophageal metastasis from prostate cancer is reported. A 65-year-old man presented with anorexia and back pain. Upper gastrointestinal X-ray fluoroscopy and endoscopy revealed a shallow longitudinal ulcer, with converging mucosal folds, approximately 5 cm above the esophagogastric junction. The histological diagnosis of the biopsied specimen was adenocarcinoma. Blood biochemistry revealed elevated serum prostatespecific antigen (PSA) and γ-seminoprotein levels. Ultrasonography of the prostate disclosed a hypoechoic lesion in the left lobe, and needle biopsy led to the diagnosis of prostatic adenocarcinoma. Since there was no finding suggestive of a primary lesion, apart from that in the prostate, we conducted reverse transcriptase-polymerase chain reaction (RT-PCR) for PSA. PSA-positive mRNA was demonstrated in the tissue of the esophageal tumor. There are three reports on metastasis to the esophagus from prostate cancer, but this is the first case of esophageal metastasis from prostate cancer without any evidence of metastasis to other organs. The importance of RT-PCR for the diagnosis of primary lesions of metastatic cancer is discussed.

Glassy Cell Carcinoma of the Uterine Cervix

Two cases of glassy cell carcinoma which is considered to be a poorly differentiated mixed adenosquamous cell carcinoma in the uterine cervix are described. Its cytologic and histologic findings are distinctive. The tumor cells had moderately amount and ground-glass cytoplasms, and had large nuclei containing a prominent nucleoli.

Enhancing effect of cholecystectomy on colon carcinogenesis induced by methylazoxymethanol acetate in hamsters

The effect of cholecystectomy on colon carcinogenesis induced by methylazoxymethanol (MAM) acetate was examined in four groups of Syrian golden hamsters. For the sexes combined, the incidences of total large intestinal neoplasms and adenomas in Group 1, which received cholecystectomy and a single intravenous injection of MAM acetate (20 mg/kg body weight), were significantly higher than those of hamsters in Group 2, which were given MAM acetate alone. The combined multiplicities of total large intestinal neoplasms from male and female hamsters, and the multiplicities of those in females of Group 1 were also significantly higher than those in animals in Group 2, respectively. No intestinal tumors were observed in hamsters in Group 3 (cholecystectomy alone) or Group 4 (untreated control). These results indicate an enhancing effect of cholecystectomy on MAM acetate-induced large intestinal carcinogenesis in hamsters

メチシリン耐性黄色ブドウ球菌 (MRSA) の検出: 気道と消化管との関連性

The study was conducted to elucidate the possibility of hospital infection of methicillin-resistant Staphylococcus aureaus (MRSA) through feces. Fecal cultures of 12 inpatients positive for MRSA in the respiratory tract and 11 inpatients negative for MRSA in the respiratory tract were performed from April to October in 1993. Fecal cultures of inpatients positive for MRSA in the respiratory tract were continued until September in 1996, finally a total of 50 cases were enrolled. MRSA was isolated from feces of 7 patients (58.3%) of the 12 patients positive for MRSA in the respiratory tract, while no MRSA was isolated from feces of 11 patients negative for MRSA in the respiratory tract. Twenty-seven patients (54.0%) of 50 patients positive for MRSA in the respiratory tract yielded MRSA in the feces. Twenty-three patients (85.2%) of the 27 patients positive for MRSA in the fecal flora had received H2-blocker, while 11 patients (47.8%) of the 23 patients negative for MRSA in the fecal flora received H2-blocker; these differences were statistically significantly (p < 0.01). These findings suggest the possible role of feces in hospital infection with MRSA and the necessity for careful administration of H2-blocker to patients positive for MRSA in the respiratory tract.

Diagnostic evaluation of multi-parameter analysis in fine needle aspiration biopsy cytology of the thyroid.

各甲状腺疾患について, 細胞診所見に加えて核内細胞質封入体, 細胞核長径, 顕微分光測光法による核DNA量および細胞総蛋白量の計測を行った.核長径は, 乳頭癌11例で, 7.8±1.4μ (mean±SD) から9.9±1.6μ, 濾胞癌で, 8.3±1.3μ および9.4±1.9μ, 未分化癌で, 10.7±2.9μ, 髄様癌で8.5±1.6μ, 9.0±1.8μ といずれも, 腺腫, 腺腫様甲状腺腫に比し増大したが, 慢性甲状腺炎でも, その多形性を反映し増大傾向が示された.核内細胞質封入体は, 良性疾患, 濾胞癌には認められず, 乳頭癌11例中10例, 髄様癌2例中1例, また, 未分化癌はその1例に陽性を示した.乳頭癌において, 癌細胞に占めるその出現頻度は, それぞれ0.4%から2.6%で, 出現頻度に関して他型のそれと有意差はなかった.核DNA量: 細胞総蛋白量は, 乳頭癌で, 計測全例の平均が2.51±0.90AU: 4.01±2.35AU, 濾胞癌で2.72±0.79AU: 2.78±0.99AU, 未分化癌で6.20±4.39AU: 6.70±4.81AUと, 腺腫例の平均2.28±0.65AU: 2.91±1.37AUに比較し, 濾胞癌で細胞総蛋白量の減少を認めた以外はいずれも有意 (P<0.001) に増加した.これらの客観的パラメーターの解析は, 甲状腺腫瘍の鑑別診断に有意義と考えられる.