Hiroyasu Misumi

The measurement of swimming velocity of Vibrio cholerae and Pseudomonas aeruginosa using the video tracking method

The swimming velocities of two monotrichous flagellated bacteria were measured by a computer‐assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3‐sec path were 75.4 ±9.4 μm/sec in four strains of Vibrio cholerae and 513 ±8.4 μm/sec in five strains of Pseudomonas aeruginosa. These results suggest that V. cholerae swim faster than P. aeruginosa at 30 C in nutrient broth. This method is useful for a detailed analysis of bacterial movement and moving patterns in different environmental conditions.

A Comparative Study of Ofloxacin Twice and Three Times Daily in the Treatment of Respiratory Tract Infections

~-Streptococci 2 4 Staphylococcus 6 2 aureus Haemophilus 2 5 influenzae Pseudomonas 2 3 aeruginosa Other 4 5 Normal flora 12 11 a Mean ± standard deviation. Abbreviations: BID = twice daily; TID = three times daily. In Japan, ofloxacin 100 to 200mg three times daily is the usual dosage regimen in the treatment of RTIs, while in Europe 200mg twice daily is the usual regimen, based on its elimination half-life of about 4 hours. We performed a study comparing ofloxacin 200mg twice daily (BID group) vs 200mg three times daily (TID group), in the treatment of RTIs.