Hiroyo Deguchi

Nuclear localization of beta-catenin involved in precancerous change in oral leukoplakia

BackgroundOral leukoplakia is a precancerous change developed in the oral mucosa, and the mechanism that oral leukoplakia becomes malignant through atypical epithelium is not known. Here we compared the β-catenin expression detected by immunohistochemical staining in the normal oral epithelium and in the oral leukoplakia with or without dysplasia.ResultsThe normal oral epithelium showed β-catenin expression only in the cell membrane, but not in the nuclei. In the oral leukoplakia without dysplasia, 7 out of 17 samples (41%) showed β-catenin expression in the cell membrane, and 5 samples (29%) showed expression in the nuclei. In the oral leukoplakia with dysplasia, nuclear expression of β-catenin was shown in 11 out of 12 samples (92%). Incidence of nuclear β-catenin expression was significantly different between dysplasia and normal oral epithelium (P < 0.01), and also between oral leukoplakia with dysplasia and those without dysplasia (P < 0.01). Wnt3 expression was detected in the epithelial cell membrane or cytoplasm in oral leukoplakia where nuclear expression of β-catenin was evident, but not in epithelial cells without nuclear expression of β-catenin.ConclusionThe components of canonical Wnt pathway, such as Wnt3, β-catenin, and cyclin D1, were detected, implying that this pathway is potentially involved in the progression of dysplasia in oral leukoplakia.

Lung adenosquamous carcinoma with lingual and cardiac metastases

Abstract Metastatic lingual tumors are very rare, and hematogenous myocardial metastasis is also relatively uncommon. We report a case of lung adenosquamous carcinoma with metastases to the tongue and the myocardium. A 65-year-old man underwent a partial upper lobectomy for a primary lung cancer in June 1997. He developed a local recurrence and received two courses of radiotherapy, in March and September 1998. A follow-up computed tomography (CT) scan showed a myocardial tumor, suggestive of cardiac metastasis. In October 1998, he began to complain of angina-like chest oppression and showed ischemic changes on electrocardiogram (ECG), and he also developed lingual, subcutaneous, and brain metastases. He died of respiratory failure in December 1998, 20 months after the initial diagnosis of primary lung cancer; metastases to the tongue and myocardium were confirmed by autopsy.