Hiroyuki Kamogawa

The possible analgesic effect of soft-laser irradiation on heat nociceptors in the cat tongue.

These effects were studied by recording single-fibre discharges in the lingual nerve. A total of 11 heat nociceptors were tested and of these 60 per cent decreased their firing frequency following soft-laser (GaA1As semiconductor laser) irradiation for 1 min; firing frequency was decreased in all the nociceptors studied following irradiation for 3 to 10 min. The reduction in firing rate was similar between 5 and 10 min after irradiation suggesting the effect reached a plateau within this time. These findings suggest that soft-laser irradiation might have an analgesic effect on tongue pain.

Candidate interneurons mediating peripherally evoked disynaptic inhibition of masseter motoneurons of both sides

Location and axonal projection of interneurons presumed to mediate disynaptic inhibition evoked from the trigeminal sensory nerve in the ipsi- and contralateral masseter motoneurons were studied in pentobarbital anesthetized cats. Neurons monosynaptically excited from the periphery and antidromically activated from the contralateral trigeminal motor nucleus at low current intensity, hence probably terminating there, were found in the supratrigeminal region. Intracellular staining of such cells revealed collaterals terminating in the ipsilateral masseter motor nucleus. It is suggested that both the crossed and uncrossed disynaptic inhibition of masseter motoneurons are at least in part relayed by the same neurons in the supratrigeminal region.

Supra- and juxtatrigeminal inhibitory premotor neurons with bifurcating axons projecting to masseter motoneurons on both sides.

Inhibitory neurons participating in the bilateral disynaptic inhibition of jaw-closing motoneurons by stimulation of unilateral trigeminal sensory branches were searched for in the reticular formation around the trigeminal motor nucleus in cats anaesthetized with pentobarbital. Extracellular recordings were made from neurons which responded orthodromically after a monosynaptic latency to single shock stimulation of the ipsilateral infraorbital and/or inferior alveolar nerves. Direct inhibitory connection with contralateral masseter motoneurons was demonstrated in reticular neurons by the spike-triggered averaging technique, i.e., by averaging the intracellular potentials of a contralateral masseter motoneuron with respect to spontaneously occurring spikes of a reticular interneuron. By intraaxonal injection of neurobiotin, electrophysiologically identified inhibitory premotor reticular neurons were found to project to and to terminate in the trigeminal motor nuclei on both sides. Termination in the contralateral motor nucleus was demonstrated for four neurons that showed the peripheral input pattern stated above. The results provide hard evidence for contralaterally projecting interneurons in the reticular formation, participating in peripherally evoked disynaptic inhibition of masseter motoneurons on the contralateral side. Given the previously reported findings that the supratrigeminal region contains neurons which project to the ipsilateral motor nucleus and mediate disynaptic inhibition of masseter motoneurons, it is suggested that the supratrigeminal region contains bilaterally projecting interneurons, mediating peripherally evoked disynaptic inhibition of masseter motoneurons on both sides.

Morphological analysis of cat masseteric motoneurons after intracellular staining with horseradish peroxidase

Intracellular injection of horseradish peroxidase (HRP) into 58 masseteric motoneurons identified by antidromic activation was performed in cats under pentobarbital anesthesia. Monosynaptic EPSPs were evoked by masseteric nerve stimuli in 52 cells, and were absent in the remaining six cells. The antidromic nature of the evoked spikes was confirmed by IS-SD separation observed at high frequency (50 Hz) stimulation. Motoneurons with monosynaptic excitation from masseter afferents showed IPSPs following stimulation of lingual and inferior alveolar nerves. Motoneurons which did not show monosynaptic excitation from masseter afferents showed no IPSPs from the above nerves. There were no differences in cell size or the number of stem dendrites between motoneurons with and without monosynaptic EPSPs. No recurrent collaterals were observed in any motor axons. Motoneurons with monosynaptic EPSPs were located at all rostrocaudal levels throughout the trigeminal motor nucleus, whereas motoneurons without such EPSPs were encountered only at the middle level. Dendrites of motoneurons with monosynaptic EPSPs did not extend into the medial portion of the nucleus where motoneurons innervating the anterior belly of the digastric muscle were located. In contrast, motoneurons without monosynaptic EPSPs had dendrite branches extending well into the medial part. The results show that there are two subpopulations of masseteric motoneurons that differ in peripheral inputs as well as dendritic morphology.

The location of brainstem neurons with bilateral projections to the motor nuclei of jaw openers in the cat

Symmetrical motor output is the rule in the masticatory system. We examined morphologically how this functional symmetry might be reflected in the organization of premotor neurons that could mediate excitation of jaw-opener motoneurons. Premotor neurons projecting bilaterally to jaw-opener motoneurons by way of axon collaterals were identified by retrograde dual-labeling with cholera toxin B-conjugated fluorescein isothiocyanate (CTb-FITC) and tetramethylrhodamine (TMR). In each cat, CTb-FITC and TMR were injected into the digastric motoneuron pools, respectively, on the left and right sides. In three animals, 69-147 neurons were labeled with both tracers, comprising approximately 44% of all retrogradely labeled cells. Double-labeled cells were located bilaterally in the trigeminal oral nucleus (Vo) and the adjacent reticular formation (RF), the former containing a larger number of cells. Neurons labeled with only one tracer were also distributed bilaterally in the Vo and RF. The results indicated that the bilaterally projecting premoter neurons identified mainly in the Vo and RF represent neuronal substrates for the symmetry that characterizes most jaw movements.

1646 Changes in masticatory movements induced by a lesion in the first somatosensory (SI) or masticatory cortex (MA) in conscious cats

Direct projections from the dorsal (PMd) and ventral (PMv) divisions of the premotor cortex to the subthalamic nucleus (SIN) were investigated in the Japanese monkey (Mucacufuscara) by using double anterograde tracing with WGAHRP and biotinylated dextran amine (BDA). Under the guidance of intracortical microstimulation, WGA-IIRP was injected into the forelimb region of PMd, and BDA was injected into the forelimb region of PMv. Anterogradely-labeled axon terminals from PMd and PMv were distributed mainly in the medial half of STN, and their distribution areas were overlapped with each other. These areas further corresponded well to the terminal field of the corticosubthalamic projection from the supplementary motor area @MA). Some labeled axon terminals from PMd and PMv were also found in the lateral half of STN that received projection fibers from the primary motor cortex. The present results indicate that the medial SIN receives convergent inputs from PMd, PMv and SMA, and support our previous observation that STN has double somatotopical representation in both medial and lateral STN.