Hiroyuki Osada

Identification of Protein–Small Molecule Interactions by Chemical Array

Molecular biology techniques have enabled us to understand the structure and function of genes and proteins. However, many phenomena, such as intracellular protein–protein interactions in cells, remain unsolved. If a specific inhibitor of a protein is available, we can describe complex biological systems that have been considered intractable. To identify drug seeds and bioprobes, the RIKEN Natural Products Depository (NPDepo) was developed to use in screens, based on the original chemical library platform, as described here. In this chapter, we introduce a screen for identifying small molecule inhibitors using the newly developed chemical array in NPDepo.