Hiroyuki Ueyama | Researchain

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Analyst | 2002

Tetrakis-acridinyl peptide: A novel fluorometric reagent for nucleic acid analysis based on the fluorescence dequenching upon DNA binding

Hiroyuki Ueyama; Makoto Takagi; Shigeori Takenaka

Tetrakis-acridinyl peptide 1 assuming a right-handed helical stacked conformation of the acridinyl units can bind to DNA duplex with a very high affinity of 10(8) (M-bp)(-1) even at a high salt concentration of 0.2 M NaCl, irrespective of the base composition. Peptide 1 gave rise to 1600-times enhancement of its fluorescence upon binding to an AT polymer due to the collapse of this stacked structure, but the fluorescence is not enhanced with a GC polymer, though the binding constants of 1 for both polymers were the same.

Organic and Biomolecular Chemistry | 2005

Fluorescence enhancement of bis-acridine orange peptide, BAO, upon binding to double stranded DNA

Keiji Mizuki; Yutaka Sakakibara; Hiroyuki Ueyama; Takahiko Nojima; Michinori Waki; Shigeori Takenaka

Bis-acridine orange peptides carrying two acridine oranges at the epsilon-amino moieties of both terminal lysines of a tetra(lysine) chain showed a ca. 200-fold fluorescence enhancement upon addition of double stranded DNA.

Analyst | 2004

Bis-intercalation-triggered fluorescence: specific detection of double stranded DNA and AT content estimation.

Hiroyuki Ueyama; Keiji Mizuki; Takahiko Nojima; Shigeori Takenaka

Detection of double stranded DNA and estimation of the AT content in DNA of unknown concentration could be achieved with a bis-acridinyl peptide carrying fluorescein, FKA.

Journal of the American Chemical Society | 2002

A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation.

Hiroyuki Ueyama; Makoto Takagi; Shigeori Takenaka

Analytical and Bioanalytical Chemistry | 2003

Comparison of potassium ion preference of potassium-sensing oligonucleotides, PSO-1 and PSO-2, carrying the human and Oxytricha telomeric sequence, respectively

Shigeori Takenaka; Hiroyuki Ueyama; Takahiko Nojima; Makoto Takagi

Analyst | 2002

Tetrakis-acridinyl peptide: A novel fluorometric reagent for nucleic acid analysis based on the fluorescence dequenching upon DNA bindingElectronic supplementary information (ESI) available: Synthetic method and data for tetrakis-acridinyl peptide 1 and monomer 2 and spectroscopic data (7 figures). See http://www.rsc.org/suppdata/an/b2/b204019k/

Hiroyuki Ueyama; Makoto Takagi; Shigeori Takenaka

Nucleic acids research. Supplement (2001) | 2001

DNA binding behavior of peptides carrying acridinyl units: First example of effective poly-intercalation.

Hiroyuki Ueyama; Makoto Takagi; Michinori Waki; Shigeori Takenaka

Nucleic Acids Symposium Series | 2000

Novel synthesis of a tetra-acridinyl peptide as a new DNA polyintercalator

Hiroyuki Ueyama; Michinori Waki; Makoto Takagi; Shigeori Takenaka

Nucleic acids research. Supplement (2001) | 2003

Fluoreometric behavior of a novel bis-acridine orange bound to double stranded DNA

Shigeori Takenaka; Yutaka Sakakibara; Hiroyuki Ueyama; Takahiko Nojima

Nucleic acids research. Supplement (2001) | 2002

Patassium sensing oligonucleotide, PSO, based on DNA tetraplex formation

Takahiko Nojima; Hiroyuki Ueyama; Makoto Takagi; Shigeori Takenaka

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