Hisami Shiraishi
Nihon University
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Publication
Featured researches published by Hisami Shiraishi.
American Journal of Reproductive Immunology | 1996
Satoshi Hayakawa; Hisami Shiraishi; Shigeru Saitoh; Kazuo Satoh
PROBLEM: Decidual tissue is an interface between the host and fetus, which is regarded as a natural allograft. We have reported the possible presence of extrathymic T cells in human decidua through the expression of recombinase‐activating genes in the decidual CD16− CD56bright large granular lymphocytes.
Immunology and Cell Biology | 1996
Satoshi Hayakawa; Meijin Tochigi; Fumihisa Chishima; Hisami Shiraishi; Noriko Takahashi; Koh Watanabe; Kiyoshi T. Fujii; Kazuo Satoh
The recombinase activation genes, RAG‐1 and RAG‐2, are expressed together in immature T or B lymphocytes and possess activity to induce V(D)J rearrangement in T cell receptor (TCR) and Ig genes. In vertebrates, only Ig and TCR molecules are reported to have recombination in their development using multiple V, D, J component gene segments. Thus, expression of RAG genes are localized only in lymphoid organs and sites of extrathymic T cell differentiation. In this study, we have used RAG‐1 and RAG‐2 genes as markers of possible genetic recombination in developing murine preimplanation embryos, using the highly sensitive reverse transcriptase polymerase chain reaction (RT‐PCR) technique and in situ hybridization. From 40 preimplantation embryos of various developmental stages we extracted RNA. reverse‐transcribed it into cDNA and used it in RT‐PCR studies. A PCR of 35 cycles disclosed expression of RAG‐1 but not RAG‐2 in morulae and blastocysts. Southern blot hybridization using a specific synthetic oligonucleotide probe for RAG‐1 and RT‐PCR with another primer pair identified RAG‐1 expression in developing embryos. In situ hybridization using a cooled CCD camera also revealed localization of RAG‐1 mRNA in blastocysts. We propose possible genetic recombination during late preimplantation murine embryogenesis which may contribute to the loss of totipotency and differentiation of inner cell mass and trophoectoderm.
Journal of Immunology | 1994
Satoshi Hayakawa; Shigeru Saito; Norimichi Nemoto; Fumihisa Chishima; K Akiyama; Hisami Shiraishi; Junko Hayakawa; Miki Karasaki-Suzuki; Kiyoshi T. Fujii; Motohiko Ichijo
Journal of clinical & laboratory immunology | 1996
Hisami Shiraishi; Satoshi Hayakawa; Kazuo Satoh
Proceedings of Annual Meeting of JSIR | 1997
Takashi Kanaeda; Satoshi Hayakawa; Fumihisa Chishima; Hisami Shiraishi; Miki Suzuki; Norimichi Nemoto; Isamu Sakurai
Proceedings of Annual Meeting of JSIR | 1997
Satoshi Hayakawa; Chiharu Hiramine; Hisami Shiraishi; Fumihisa Chisima; Miki Karasaki-Suzuki; Tom Kiyoshi Fujii; Kiyohide Furuya; Kazuo Satoh
Proceedings of Annual Meeting of JSIR | 1997
Hisami Shiraishi; Satoshi Hayakawa; Fumihisa Chishima; Miki Karasaki-Suzuki; Maki Ishii; Nobihisa Nagai; Takashi Kaneeda; Kazuo Satoh
Proceedings of Annual Meeting of JSIR | 1996
Fumihisa Chishima; Satoshi Hayakawa; Hisami Shiraishi; Yoshiyasu Hirata; Nobuhisa Nagai; Takashi Kanaeda; Katsuo Tsubata; Kazuo Satoh
Proceedings of Annual Meeting of JSIR | 1996
Hisami Shiraishi; Satoshi Hayakawa; Toshiaki Nagaoka; Fumihia Chishima; Miki Karasaki; Yoshiaki Miyake; Kiyoshi T. Fujii; Kazuo Satoh
Placenta | 1996
Fumihisa Chishima; Satoshi Hayakawa; Hisami Shiraishi; Kazuo Satoh