Hitoshi Nakagama
Hokkaido University
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Publication
Featured researches published by Hitoshi Nakagama.
Archive | 2006
Mitsuko Masutani; Akemi Gunji; Masahiro Tsutsumi; Kumiko Ogawa; Nobuo Kamada; Tomoyuki Shirai; Kou-ichi Jishage; Hitoshi Nakagama; Takashi Sugimura
Elucidation of the relationship between poly-ADP-ribosylation and carcinogenesis has markedly progressed by the recent development of knockout or transgenic mice models of poly(ADP-ribose) polymerase (Parp)-1, Parp-2, and poly(ADP-ribose) glycohydrolase (Parg). Parp-1 is involved in base excision repair (BER), single- and |double-strand break repair, and chromosomal stability. These multiple functions explain why Parp-1 deficiency enhances carcinogenesis induced by alkylating agents and that in aged animals. Parp-1 is also involved in transcriptional regulation through protein-protein interaction as a coactivator and/or poly-ADP-ribosylation reaction and is possibly involved in epigenetic alteration during carcinogenesis and modulation of tumor phenotypes. Parp-1-dependent cell-death accompanying NAD depletion may be another important issue in carcinogenesis because this process could lead to the selection of Parp-1 deficient cells due to their survival advantage during cancer growth. The relationship of Parp-2, Parp-3, tankyrase and Parg with carcinogenesis is also discussed.
Organic Chemistry Insights | 2009
Takashi Shimokawa; Hideki Ogino; Daisuke Maeda; Hitoshi Nakagama; Takashi Sugimura; Mitsuko Masutani
Poly(ADP-ribose) polymerase (PARP) polyADP-ribosylates proteins involved in various physiological processes. Accumulated evidence suggests not only protein-conjugated poly(ADP-ribose) but also protein-free poly(ADP-ribose) function in various physiological processes. There are increasing occasions that require protein-free poly(ADP-ribose) to study the function and dynamics of poly(ADP-ribose) in cells. However, the availability of poly(ADP-ribose) is still limited because a chemical synthesis method has not been established. Here, we describe an improved method for the preparation of protein-free poly(ADP-ribose), synthesized enzymatically by using a recombinant PARP-1 expression system and purified with an anion-exchange column chromatography. This method will be useful for biochemical and biological investigation of poly(ADP-ribose) functions and dynamics.
Proceedings of the Japan Academy. Ser. B: Physical and Biological Sciences | 2001
Tadashige Nozaki; Hisako Fujihara; Nobuo Kamada; Otoya Ueda; Tsuyoshi Takato; Hitoshi Nakagama; Takashi Sugimura; Hiroshi Suzuki; Mitsuko Masutani
International Journal of Oncology | 2000
Haruki Shirato; Hiroshi Shima; Hitoshi Nakagama; Hirokazu Fukuda; Yuki Watanabe; Katsuhiro Ogawa; Yoichi Matsuda; Kunimi Kikuchi
Archive | 2010
Hitoshi Nakagama; Naoto Tsuchiya; Masashi Izumiya
Archive | 2007
Hitoshi Nakagama; Masaru Tazawa; Naoto Tsuchiya; 斉 中釜; 直人 土屋; 大 田澤
Proceedings of the Japan Academy. Series B Physical and biological sciences | 2003
Fumiaki Watanabe; Mitsuko Masutani; Nobuo Kamada; Hiroshi Suzuki; Hitoshi Nakagama; Takashi Sugimura; Hirobumi Teraoka
Proceedings of the Japan Academy. Series B Physical and biological sciences | 2003
Akemi Gunji; Hisako Fujihara; Nobuo Kamada; Ken Omura; Kou-ichi Jishage; Hitoshi Nakagama; Takashi Sugimura; Mitsuko Masutani
Archive | 2013
Naoto Tsuchiya; Hiroko Ogata; Takuji Okusaka; Hitoshi Nakagama
Proceedings of the Japan Academy. Series B Physical and biological sciences | 2003
Hirokazu Fukuda; Naoto Tsuchiya; Maiko Sato; Akira Yamaguchi; Nanae Tanaka; Minako Nagao; Hitoshi Nakagama
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Obihiro University of Agriculture and Veterinary Medicine
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