Hitoshi Satoh

High resolution chromosome banding in the Norway rat, Rattus norvegicus

High resolution banded chromosomes were prepared from a synchronized culture of rat fibroblasts. A maximum of 457 bands per haploid chromosome set were observed. This represents a two-fold increase when compared to the number of bands visualized in mid-metaphases using standard procedures. By reference to both G- and Q-banded karyotypes, we constructed improved idiograms of rat chromosomes at 300- and 400-band stages, respectively.

Chromosomal mapping of the mouse IL-4 and human IL-5 genes

We mapped the mouse interleukin (IL)-4 gene on chromosome 11 by restriction fragment length polymorphism using recombinant inbred mouse strains. The human IL-5 gene was mapped on chromosome 5q 23.3-31.1 by in situ hybridization. Because the granulocyte macrophage colony-stimulating factor (GM-CSF) and IL-3 genes were previously mapped on mouse chromosome 11 (within a 230-kb region) and human chromosome 5, the IL-4 and IL-5 genes are likely to cluster on the same chromosomes with the GM-CSF and IL-3 genes in both species.

Nucleotide Sequence and Chromosomal Mapping of the Human c‐yes‐2 Gene

We molecularly characterized the second gene, c‐yes‐2, of two copies of yes‐related genes which we previously found to contain in the human genome. First, nucleotide sequence analysis revealed that the c‐yes‐2 gene is a pseudogene of the c‐yes‐1 gene. Second, by using two independent methods, hybridization of both DNAs from sorted chromosomes and metaphase spreads with c‐yes‐2 DNA, we assigned the c‐yes‐2 gene to chromosome 22q11.2. This chromosomal localization is inconsistent with that given in our previous report. The failure of proper mapping in our experiment might have been caused by instability of hybrid cell clones.