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Dive into the research topics where Ho Sub Lee is active.

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Featured researches published by Ho Sub Lee.


Stroke | 2011

1H-NMR-based metabolomics study of cerebral infarction.

Jee Youn Jung; Ho Sub Lee; Dae-Gill Kang; No Soo Kim; Min Ho Cha; Ok-Sun Bang; Do Hyun Ryu; Geum-Sook Hwang

Background and Purpose— Stroke is one of the leading causes of adult disability and death in developing countries. However, early diagnosis is difficult and no reliable biomarker is currently available. Thus, we applied a 1H-NMR metabolomics approach to investigate the altered metabolic pattern in plasma and urine from patients with cerebral infarctions and sought to identify metabolic biomarkers associated with stroke. Methods— Metabolic profiles of plasma and urine from patients with cerebral infarctions, especially small vessel occlusion, were investigated using 1H-NMR spectroscopy coupled with multivariate statistical analysis, such as principal components analysis and orthogonal partial least-squares discriminant analysis. Results— Multivariate statistical analysis showed a significant separation between patients and healthy individuals. The plasma of stroke patients was characterized by the increased excretion of lactate, pyruvate, glycolate, and formate, and by the decreased excretion of glutamine and methanol; the urine of stroke patients was characterized by decreased levels of citrate, hippurate, and glycine. These metabolites detected from plasma and urine of patients with cerebral infarctions were associated with anaerobic glycolysis, folic acid deficiency, and hyperhomocysteinemia. Furthermore, the presence of cerebral infarction in the external validation model was predicted with high accuracy. Conclusions— These data demonstrate that a metabolomics approach may be useful for the effective diagnosis of cerebral infarction and for the further understanding of stroke pathogenesis.


Journal of Ethnopharmacology | 2003

Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea

Dae Gill Kang; Chi keun Yun; Ho Sub Lee

The hexane, ethylacetate, n-butanol, and water extracts of 10 Korean herbal medicines were screened and compared for their antioxidant activities in a range of lipid peroxidation system using rat brain homogenates, antihemolysis assay of red blood cells, and other in vitro assays to determine their ability to scavenge superoxide and hydroxyl radicals. All of the 10 Korean herbal medicines have potent antioxidant activities. Among the four solvent extracts, the antioxidant activities of more-polar solvent extracts (BuOH and water extracts) were relatively higher than that of non-polar solvent extracts (hexane and EtOAC extracts). These results will be useful to further analyze those herbal medicines that contain the most antioxidant activity in order to identify the active principles.


Experimental and Molecular Medicine | 2006

Comparative effects of curcuminoids on endothelial heme oxygenase-1 expression: ortho-methoxy groups are essential to enhance heme oxygenase activity and protection

Gil-Saeng Jeong; Gi-Su Oh; Hyun-Ock Pae; Sun-Oh Jeong; Youn-Chul Kim; Min-Kyo Shin; Byeong Yun Seo; Sang Youp Han; Ho Sub Lee; Jong-Gil Jeong; Jeong-Soon Koh; Hun-Taeg Chung

Recently, it has been reported that curcumin, which is known as a potent antioxidant, acts as a non-stressful and non-cytotoxic inducer of the cytoprotective heme oxygenase (HO)-1. In this study, naturally occurring curcuminoids, such as pure curcumin, demethoxycurcumin (DMC) and bis-demethoxycurcumin (BDMC), were compared for their potential ability to modulate HO-1 expression and cytoprotective activity in human endothelial cells. All three curcuminoids could induce HO-1 expression and HO activity with differential levels. The rank order of HO activity was curcumin, DMC and BDMC. In comparison with endothelial protection against H2O2-induced cellular injury, cytoprotective capacity was found to be highest with curcumin, followed by DMC and BDMC. Interestingly, cytoprotective effects afforded by curcuminoids were considerably associated with their abilities to enhance HO activity. Considering that the main difference among the three curcuminoids is the number of methoxy groups (none for BDMC, one for DMC, and two for curcumin), the presence of methoxy groups in the ortho position on the aromatic ring was suggested to be essential to enhance HO-1 expression and cytoprotection in human endothelial cells. Our results may be useful in designing more efficacious HO-1 inducers which could be considered as promising pharmacological agents in the development of therapeutic approaches for the prevention or treatment of endothelial diseases caused by oxidative damages.


Circulation Research | 2004

High and Low Gain Switches for Regulation of cAMP Efflux Concentration Distinct Roles for Particulate GC- and Soluble GC-cGMP-PDE3 Signaling in Rabbit Atria

Jin Fu Wen; Xun Cui; Jing Yu Jin; Soo Mi Kim; Sung Zoo Kim; Suhn Hee Kim; Ho Sub Lee; Kyung Woo Cho

Abstract— This study tests the hypothesis that particulate (p) guanylyl cyclase (GC) and soluble (s) GC are involved in the distinct roles for the regulation of cGMP-PDE-cAMP signaling and of mechanical and secretory functions in the heart. Experiments were performed in perfused beating rabbit atria. C-type natriuretic peptide (CNP) and SIN-1, an NO donor, or BAY 41-2272 (BAY), a direct activator for sGC, were used to activate pGC and sGC, respectively. CNP and SIN-1 increased cGMP and cAMP efflux in a concentration-dependent manner. Increase in cAMP was a function of cGMP. The changes in cAMP efflux concentration in terms of cGMP were much more prominent in the atria treated with CNP than in the atria treated with SIN-1. Increase in cAMP efflux concentration was blocked by milrinone but not changed by EHNA. BAY increased cGMP but not cAMP in a concentration-dependent manner. CNP and SIN-1 decreased atrial stroke volume and myocytic ANP release. The decreases in terms of cGMP efflux concentration were much more prominent in the atria treated with CNP than in the atria treated with SIN-1 or BAY. Milrinone accentuated GC agonist–induced decreases in atrial stroke volume and ANP release. In the presence of ODQ, SIN-1 or BAY induced effects were not observed. These data suggest that pGC and sGC activations have distinct roles via cGMP-PDE3-cAMP signaling in the cardiac atrium: high and low gain switches, respectively, for the regulation of cAMP levels and contractile and secretory functions.


Life Sciences | 2002

Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension.

Dae Gill Kang; Tae Young Hur; Geon Mok Lee; Hyuncheol Oh; Tae Oh Kwon; Eun Jin Sohn; Ho Sub Lee

A pharmacological inhibition of nitric oxide synthase (NOS) in rats for 4-6 weeks produces renal vasoconstriction, renal dysfunction, and severe hypertension. The present study was aimed at investigating whether Cudrania tricuspidata (C. tricuspidata) water extract ameliorates N(G)-Nitro-L-arginine methylester (L-NAME)-induced hypertension. Treatment of L-NAME (60 mg/L drinking water, 4 weeks) causes a sustained increase in systolic blood pressure (SBP). The concentration of plasma NO metabolites and NO/cGMP productions in the vascular tissues of the L-NAME-treated group were significantly reduced as compared with those in the control. C. tricuspidata water extract blocked increase of SBP in the L-NAME-treated group and restored SBP to normal level. Futhermore, C. tricuspidata water extract was able to preserve the vascular NO/cGMP production and plasma NO metabolites concentration. However, there are no changes in the expression of ecNOS and iNOS of thoracic aorta among the rats of control, L-NAME-treated group, and L-NAME and C. tricuspidata water extract co-treated group. The urinary sodium level, urine volume, and creatinine clearance were significantly higher in rats co-treated with C. tricuspidata water extract and L-NAME than in L-NAME-treated group. Taken together, these results suggest that C tricuspidata water extract prevents the increase of SBP in the L-NAME-induced hypertension that may have been caused by enhanced generation of vascular NO/cGMP.


Biochemical and Biophysical Research Communications | 2010

Protective role of betulinic acid on TNF-α-induced cell adhesion molecules in vascular endothelial cells

Jung Joo Yoon; Yun Jung Lee; Jin Sook Kim; Dae Gill Kang; Ho Sub Lee

Vascular inflammation is an important event in the development of vascular diseases such as tumor progression and atherosclerosis. In the present study, betulinic acid (BA) treatment was found to show potent inhibitory effect on vascular inflammation process by TNF-alpha in human umbilical vein endothelial cells (HUVEC). Pretreatment of HUVEC with BA was blocked TNF-alpha induced expression level of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), endothelial cell selectin (E-selectin) as well as gelatinase in TNF-alpha-activated HUVEC in a dose-dependent manner. When preincubated with BA, the adhesion of HL-60 cells to TNF-alpha-induced HUVEC was significantly decreased in a concentration-dependent manner. TNF-alpha-induced intracellular ROS was markedly decreased by pretreatment with BA. Furthermore, BA significantly inhibited the translocation and transcriptional activity of NF-kappaB increased by TNF-alpha. In conclusion, these results suggested a vascular protective role of BA via inhibition of ROS and NF-kappaB activation in HUVEC.


Journal of Ethnopharmacology | 2003

Angiotensin converting enzyme inhibitory phenylpropanoid glycosides from Clerodendron trichotomum.

Dae Gill Kang; Yong Sup Lee; Hyoung Ja Kim; Yun Mi Lee; Ho Sub Lee

The stems of Clerodendron trichotomum have been traditionally used for treatment of hypertension in far East Asia including China, Korea, and Japan. Bioassay-guided fractionation and purification of the EtOAc-soluble extract of Clerodendron trichotomum afforded acteoside (1), leucosceptoside A (2), martynoside (3), acteoside isomer (4), and isomartynoside (5). The angiotensin converting enzyme (ACE) activities were significantly inhibited by the addition of these phenylpropanoid glycosides (1-5) in a dose-dependent manner of which IC(50) values were 373+/-9.3 microg/ml, 423+/-18.8 microg/ml, 524+/-28.1 microg/ml, 376+/-15.6 microg/ml, 505+/-26.7 microg/ml, respectively. These results suggest that the antihypertensive effect of Clerodendron trichotomum may be, at least in part, due to ACE inhibitory effect of phenylpropanoid glycosides.


The American Journal of Chinese Medicine | 2011

Hypotensive, Hypolipidemic, and Vascular Protective Effects of Morus alba L. in Rats Fed an Atherogenic Diet

Yun Jung Lee; Deok Ho Choi; Eun Ju Kim; Hye Yoom Kim; Tae Oh Kwon; Dae Gill Kang; Ho Sub Lee

Morus alba L. has been used in traditional Chinese medicine and almost all parts of this plant are useful in cardiovascular, liver and spleen disorders. The present study was designed to investigate the inhibitory effect of a water extract from Morus alba L. (WMA) on vascular dysfunction in rat models fed a high fat and high cholesterol diet. Male rats were fed an atherogenic diet consisting of food with 7.5% cocoa butter and 1.25% cholesterol, with or without 100 or 200 mg/day/kg WMA, for 14 weeks. Chronic treatment with low (100 mg/kg/day) or high (200 mg/day/kg) doses of WMA markedly attenuated hypertension and the impairments of acetylcholine-induced relaxation of aortic rings in rats fed an atherogenic diet. WMA reduced intima/media thickness in rats fed an atherogenic diet. WMA improved plasma levels of triglyceride (TG) and augmented plasma levels of high-density lipoprotein (HDL) and plasma low-density lipoprotein (LDL), but did not affect blood glucose levels. Interestingly, WMA suppressed increased cell adhesion molecules such as E-selectin, vascular cell adhesion molecule-1 (VCAM-1), and intracellular adhesion molecule-1 (ICAM-1) expression in the aorta. Taken together, these results suggested that Morus alba L. could improve an atherogenic diet-induced hypertension, hyperlipidemia, and vascular dysfunction through inhibition of cell adhesion molecules expression and induction of vascular relaxation.


Immunopharmacology and Immunotoxicology | 2011

Vanillic acid inhibits inflammatory mediators by suppressing NF-κB in lipopolysaccharide-stimulated mouse peritoneal macrophages

Min-Cheol Kim; Su-Jin Kim; Dae-Seung Kim; Yong-Deok Jeon; Sung Joo Park; Ho Sub Lee; Jae-Young Um; Seung-Heon Hong

Vanillic acid is a benzoic acid derivative that is used as a flavoring agent. It is an oxidized form of vanillin. At present, the mechanisms by which vanillic acid exerts its anti-inflammatory effects are incompletely understood. In this study, we attempted to determine the effects of vanillic acid on lipopolysaccharide (LPS)-induced inflammatory responses in mouse peritoneal macrophages. Our findings indicate that vanillic acid inhibits LPS-induced production of tumor necrosis factor (TNF)-α and interleukin (IL)-6. During the inflammatory process, the levels of cyclooxygenase (COX)-2 and nitric oxide (NO) increased in mouse peritoneal macrophages, but vanillic acid suppressed both the enhanced levels of COX-2 and the production of prostaglandin E2 and NO. Moreover, vanillic acid suppressed the activation of nuclear factor-kappa B (NF-κB) and caspase-1. These results provide novel insights into the pharmacological actions of vanillic acid and are indicative of the potential use of this molecule in the treatment of inflammatory diseases.


Stress and Health | 2001

Psychoneuroimmunological effects of Qi-therapy : preliminary study on the changes of level of anxiety, mood, cortisol and melatonin and cellular function of neutrophil and natural killer cells

Myeong Soo Lee; Hwa Jeong Huh; Sung-Soo Hong; Hye-Sook Jang; Hoon Ryu; Ho Sub Lee; Hun-Taeg Chung

This preliminary study investigated the psychoneuroimmunological effects of Korean Qi-therapy (QT) on randomly divided placebo group (N = 10) and QT group (N = 10) via measuring the level of anxiety, mood, cortisol and melatonin, and the cellular function of neutrophil and NK cells. Although the basal levels of anxiety and mood were not different between the two groups, there were significant differences in group by time interaction in the anxiety level (5 min after intervention, Post I: changed by −23 per cent in QT group and −10 per cent in placebo; 1 hour after, Post II: −23 per cent, −8 per cent) and mood score (Post I: −34 per cent, −14 per cent; Post II: −55 per cent, −21 per cent). Melatonin levels also changed differently by intervention. In response to QT, melatonin levels increased after treatment but decreased in the control. For neutrophil response to intervention, superoxide generation was increased by QT but decreased by placebo (group by time interaction, p < 0.0001; changed by 36 per cent in the QT group and 8 per cent in the placebo group). There was a significant change in NK cell cytotoxicity in the QT group. The cytotoxicity increased (27 per cent compared to baseline) in the QT group but there were no changes in the placebo group (7 per cent). Our current observations suggest that Korean Qi-therapy may induce psychological stabilization, increase melatonin level and enhance cellular function of neutrophil and NK cell. Therefore Qi-therapy may be an effective complementary method for human health care and in the prevention of disease. Copyright

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