Holger Birk
Leica Microsystems
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Publication
Featured researches published by Holger Birk.
Confocal, Multiphoton, and Nonlinear Microscopic Imaging (2003), paper 5139_147 | 2003
Holger Birk; Rafael Storz; Heinrich Ulrich
Due to the tendencies in biological research towards multiple stainings and increased use of newly developed dyes like fluorescent proteins, the application areas of multispectral confocal imaging increase rapidly. We present here the most recent advances in this technology particularily concerning but not limited to the excitation module, the detection module, and the beamsplitter optics of multispectral confocal microscopes.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX | 2002
Holger Birk; Johann Engelhardt; Rafael Storz; Nicole Hartmann; Joachim Bradl; Heinrich Ulrich
In confocal microscopy manifold combinations of dyes are used. The entire wavelength range from near-ultraviolet to near-infrared is used to excite these dyes. Their emission is then detected primarily in the visible range. In addition to the number of dyes, the number of possible excitation laser lines is already large and constantly increasing. Due to the almost unlimited number of possible combinations, programmable devices are required. This is true for excitation-modules, beam-splitters and detection modules. Systems using filters with fixed spectral properties can practically fulfill the requirements for only a very small subset of applications. Programmable devices have been realized for excitation using Acousto Optical Tunable Filters (AOTFs). Freely definable spectral detectors are available for a perfect adaptation to the desired emission bands. The missing link for a completely filter-free design is now introduced using an Acousto Optical Beam Splitter (AOBS).
Archive | 2001
Holger Birk; Rafael Storz
Archive | 2001
Holger Birk; Rafael Storz; Johann Engelhardt
Archive | 2003
Werner Knebel; Holger Birk; Rafael Storz
Archive | 2005
Holger Birk; Rafael Storz
Archive | 2001
Holger Birk; Rafael Storz; Johann Engelhardt; Kyra Moellmann
Archive | 2004
Holger Birk; Rafael Storz; Johann Engelhardt; Kyra Moellmann
Archive | 2001
Holger Birk; Rafael Storz; Johann Engelhardt
Archive | 2012
Holger Birk