Hongjie Guo
Ohio State University
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Featured researches published by Hongjie Guo.
Nature Chemical Biology | 2010
Robert Woodward; Wen Yi; Lei Li; Guohui Zhao; Hironobu Eguchi; Perali Ramu Sridhar; Hongjie Guo; Jing Katherine Song; Edwin Motari; Li Cai; Patrick Kelleher; Xianwei Liu; Weiqing Han; Wenpeng Zhang; Yan Ding; Mei Li; Peng George Wang
Polysaccharides constitute a major component of bacterial cell surfaces and play critical roles in bacteria/host interactions. The biosynthesis of such molecules, however, has mainly been characterized through in vivo genetic studies, thus precluding discernment of the details of this pathway. Accordingly, we present a chemical approach which enabled reconstitution of the E. coli O-polysaccharide biosynthetic pathway in vitro. Starting with chemically prepared N-Acetyl-D-galactosamine-diphospho-undecaprenyl, the E. coli O86 oligosaccharide repeating unit was assembled via sequential enzymatic glycosylation. Successful expression of the putative polymerase Wzy via a chaperone co-expression system then allowed demonstration of polymerization in vitro using this substrate. Analysis of additional substrates revealed a defined mode of recognition for Wzy towards the lipid moiety. Specific polysaccharide chain length modality was furthermore demonstrated to result from the action of Wzz. Collectively, polysaccharide biosynthesis was chemically reconstituted in vitro, providing a well-defined system for further underpinning molecular details of this biosynthetic pathway.
Current Topics in Medicinal Chemistry | 2008
Peng George Wang; Hongjie Guo; Wen Yi; Jing K. Song
The surfaces of almost all microbes are decorated with remarkable variations of polysaccharides such as O-antigen, capsular polysaccharides (CPS), and exopolysaccharides (EPS) in bacteria, lipoarabinomannans (LAM) in mycobacteria and lipophosphoglycan (LPG) in Leishmania. These polysaccharides play important roles in many biological processes, and they can function as the virulence determinants in the pathogens. The basic structures of these polysaccharides are known, but they show species-specificity or stage-specificity. For example, there are 186 O-serotypes and 80 capsular serotypes in E. coli. Despite the variation, the range of strategies used for the biosynthesis and assembly of these microbial polysaccharides is limited. Depending on the assembly and translocation mechanisms, O-antigen biosynthesis is subdivided into three pathways, of which the Wzy-dependent pathway is widely used not only in O-antigen, but also in CPS and EPS.
Applied and Environmental Microbiology | 2005
Hongjie Guo; Wen Yi; Jun Shao; Yuquan Lu; Wenpeng Zhang; Jing Song; Peng George Wang
ABSTRACT Escherichia coli O86:B7 has long been used as a model bacterial strain to study the generation of natural blood group antibody in humans, and it has been shown to possess high human blood B activity. The O-antigen structure of O86:B7 was solved recently in our laboratory. Comparison with the published structure of O86:H2 showed that both O86 subtypes shared the same O unit, yet each of the O antigens is polymerized from a different terminal sugar in a different glycosidic linkage. To determine the genetic basis for the O-antigen differences between the two O86 strains, we report the complete sequence of O86:B7 O-antigen gene cluster between galF and hisI, each gene was identified based on homology to other genes in the GenBank databases. Comparison of the two O86 O-antigen gene clusters revealed that the encoding regions between galF and gnd are identical, including wzy genes. However, deletion of the two wzy genes revealed that wzy in O86:B7 is responsible for the polymerization of the O antigen, while the deletion of wzy in O86:H2 has no effect on O-antigen biosynthesis. Therefore, we proposed that there must be another functional wzy gene outside the O86:H2 O-antigen gene cluster. Wzz proteins determine the degree of polymerization of the O antigen. When separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the lipopolysaccharide (LPS) of O86:B7 exhibited a modal distribution of LPS bands with relatively short O units attached to lipid A-core, which differs from the LPS pattern of O86:H2. We proved that the wzz genes are responsible for the different LPS patterns found in the two O86 subtypes, and we also showed that the very short type of LPS is responsible for the serum sensitivity of the O86:B7 strain.
Journal of the American Chemical Society | 2005
Wen Yi; Jun Shao; Lizhi Zhu; Mei Li; Mamata Singh; Yuquan Lu; Steven Lin; Hanfen Li; Kang Ryu; Jie Shen; Hongjie Guo; Qingjia Yao; and C. Allen Bush; Peng George Wang
Biochemistry | 2007
Kuo-Hsiang Tang; Hongjie Guo; Wen Yi; Ming-Daw Tsai; Peng George Wang
Protein Expression and Purification | 2006
Hongjie Guo; Kaarina Lokko; Yun Zhang; Wen Yi; Zhengrong Wu; Peng George Wang
Biochemistry | 2006
Hongjie Guo; Lei Li; Peng George Wang
Biochemical and Biophysical Research Communications | 2007
Hongjie Guo; Wen Yi; Lei Li; Peng George Wang
Carbohydrate Research | 2006
Wen Yi; Peter Bystricky; Qingjia Yao; Hongjie Guo; Lizhi Zhu; Hanfen Li; Jie Shen; Mei Li; Soumya Ganguly; C. Allen Bush; Peng George Wang
Carbohydrate Research | 2006
Wen Yi; Lizhi Zhu; Hongjie Guo; Mei Li; Jianjun Li; Peng George Wang