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Featured researches published by Honglan Yang.


Frontiers in Plant Science | 2015

Characterization of reference genes for RT-qPCR in the desert moss Syntrichia caninervis in response to abiotic stress and desiccation/rehydration

Xiaoshuang Li; Daoyuan Zhang; Haiyan Li; Bei Gao; Honglan Yang; Yuanming Zhang; Andrew J. Wood

Syntrichia caninervis is the dominant bryophyte of the biological soil crusts found in the Gurbantunggut desert. The extreme desert environment is characterized by prolonged drought, temperature extremes, high radiation and frequent cycles of hydration and dehydration. S. caninervis is an ideal organism for the identification and characterization of genes related to abiotic stress tolerance. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) expression analysis is a powerful analytical technique that requires the use of stable reference genes. Using available S. caninervis transcriptome data, we selected 15 candidate reference genes and analyzed their relative expression stabilities in S. caninervis gametophores exposed to a range of abiotic stresses or a hydration-desiccation-rehydration cycle. The programs geNorm, NormFinder, and RefFinder were used to assess and rank the expression stability of the 15 candidate genes. The stability ranking results of reference genes under each specific experimental condition showed high consistency using different algorithms. For abiotic stress treatments, the combination of two genes (α-TUB2 and CDPK) were sufficient for accurate normalization. For the hydration-desiccation-rehydration process, the combination of two genes (α-TUB1 and CDPK) were sufficient for accurate normalization. 18S was among the least stable genes in all of the experimental sets and was unsuitable as reference gene in S. caninervis. This is the first systematic investigation and comparison of reference gene selection for RT-qPCR work in S. caninervis. This research will facilitate gene expression studies in S. caninervis, related moss species from the Syntrichia complex and other mosses.


Plant Physiology and Biochemistry | 2015

Ectopic overexpression of the aldehyde dehydrogenase ALDH21 from Syntrichia caninervis in tobacco confers salt and drought stress tolerance.

Honglan Yang; Daoyuan Zhang; Haiyan Li; Lingfeng Dong; Haiyan Lan

Aldehyde dehydrogenases are important enzymes that play vital roles in mitigating oxidative/electrophilic stress when plants are exposed to environmental stress. An aldehyde dehydrogenase gene from Syntrichia caninervis, ScALDH21, was introduced into tobacco using Agrobacterium-mediated transformation to generate ScALDH21-overexpressing tobacco plants to investigate its effect on drought and salt resistance. Detached leaves from ScALDH21-overexpressing tobacco plants showed less water loss than those from nontransgenic plants. When subjected to drought and salt stress, transgenic plants displayed higher germination ratios, higher root lengths, greater fresh weight, higher proline accumulation, lower malondialdehyde (MDA) contents and stronger photosynthetic capacities, as well as higher activities of antioxidant enzymes, i.e., superoxide dismutase, catalase and peroxidase, compared with control plants. Therefore, ScALDH21 overexpression in transgenic tobacco plants can enhance drought and salt tolerance and can be used as a candidate gene for the molecular breeding of salt- and drought-tolerant plants.


International Journal of Molecular Sciences | 2012

Reference Gene Selection in the Desert Plant Eremosparton songoricum

Xiaoshuang Li; Honglan Yang; Daoyuan Zhang; Yuan-Ming Zhang; Andrew J. Wood

Eremosparton songoricum (Litv.) Vass. (E. songoricum) is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various E. songoricum plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions. EsEF and Esα-TUB were sufficient for various stress conditions, EsEF and EsACT were suitable for samples of differing germination stages, and EsGAPDHand EsUBQ were most stable across multiple adult tissue samples. The Es18S gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor EsDREB2 verified the utility of E. songoricum reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in E. songoricum, and these data will facilitate future work on gene expression in this species.


Journal of Plant Physiology | 2016

Novel DREB A-5 subgroup transcription factors from desert moss (Syntrichia caninervis) confers multiple abiotic stress tolerance to yeast ☆

Haiyan Li; Daoyuan Zhang; Xiaoshuang Li; Kaiyun Guan; Honglan Yang

Syntrichia caninervis Mitt. is a typical desiccation tolerant moss from a temperate desert which has been a good resource for stress tolerant gene isolation. Dehydration responsive element binding proteins (DREBs) was proven to play an important role in responding to abiotic stress, which has been identified in many plants, and were rarely reported in moss. In this study, we cloned ten DREB genes from S. caninervis, and investigated their abiotic stress response and stress tolerance. The results showed that ten ScDREB proteins belonged to the A-5 sub-group of the DREB sub-family. Six genes, ScDREB1, ScDREB2, ScDREB4, ScDREB6, ScDREB7, and ScDREB8 were involved in the ABA-dependent signal pathway and the desiccation, salt, and cold stress response. ScDREB3 also responded to desiccation, salt, and cold stresses, but was insensitive to ABA treatment. Another gene, ScDREB5, was involved in an ABA-independent cold stress-responsive signal pathway. Two genes, ScDREB9 and ScDREB10, responded slightly or had no response to neither stress factor or ABA treatment. We transformed four typical genes into yeast cells and the stress tolerance ability of transgenic yeast was evaluated. The results showed that ScDREB3 and ScDREB5 enhanced the yeasts cold and salt tolerance. ScDREB8 and ScDREB10 conferred the osmotic, salt, cold, and high temperature stresses tolerance, especially for osmotic and salt stresses. Our results indicated that A-5 sub-group DREB genes in S. caninervis played important roles in abiotic stresses response and enhanced stress tolerance to transgenic yeast. To our knowledge, this is the first report on DREB genes characterization from desiccation tolerant moss, and this study will not only provide insight into the molecular mechanisms of S. caninervis adaptation to environmental stresses, but also provides valuable gene candidates for plant molecular breeding.


Frontiers in Plant Science | 2017

Transcriptome-Wide Identification, Classification, and Characterization of AP2/ERF Family Genes in the Desert Moss Syntrichia caninervis

Xiaoshuang Li; Daoyuan Zhang; Bei Gao; Yuqing Liang; Honglan Yang; Yucheng Wang; Andrew J. Wood

APETALA2/Ethylene Responsive Factor (AP2/ERF) is a large family of plant transcription factors which play important roles in the control of plant metabolism and development as well as responses to various biotic and abiotic stresses. The desert moss Syntrichia caninervis, due to its robust and comprehensive stress tolerance, is a promising organism for the identification of stress-related genes. Using S. caninervis transcriptome data, 80 AP2/ERF unigenes were identified by HMM modeling and BLASTP searching. Based on the number of AP2 domains, multiple sequence alignment, motif analysis, and gene tree construction, ScAP2/ERF genes were classified into three main subfamilies (including 5 AP2 gene members, 72 ERF gene members, and 1 RAV member) and two Soloist members. We found that the ratio for each subfamily was constant between S. caninervis and the model moss Physcomitrella patens, however, as compared to the angiosperm Arabidopsis, the percentage of ERF subfamily members in both moss species were greatly expanded, while the members of the AP2 and RAV subfamilies were reduced accordingly. The amino acid composition of the AP2 domain of ScAP2/ERFs was conserved as compared with Arabidopsis. Interestingly, most of the identified DREB genes in S. caninervis belonged to the A-5 group which play important roles in stress responses and are rarely reported in the literature. Expression profile analysis of ScDREB genes showed different gene expression patterns under dehydration and rehydration; the majority of ScDREB genes demonstrated a stronger response to dehydration relative to rehydration indicating that ScDREB may play an important role in dehydrated moss tissues. To our knowledge, this is the first study to detail the identification and characterization of the AP2/ERF gene family in a desert moss. Further, this study will lay the foundation for further functional analysis of these genes, provide greater insight to the stress tolerance mechanisms in S. caninervis and provide a reference for AP2/ERF gene family classification in other moss species.


Scientific Reports | 2017

Desiccation tolerance in bryophytes: The dehydration and rehydration transcriptomes in the desiccation-tolerant bryophyte Bryum argenteum

Bei Gao; Xiaoshuang Li; Daoyuan Zhang; Yuqing Liang; Honglan Yang; Mo-Xian Chen; Yuanming Zhang; Jianhua Zhang; Andrew J. Wood

The desiccation tolerant bryophyte Bryum argenteum is an important component of desert biological soil crusts (BSCs) and is emerging as a model system for studying vegetative desiccation tolerance. Here we present and analyze the hydration-dehydration-rehydration transcriptomes in B. argenteum to establish a desiccation-tolerance transcriptomic atlas. B. argenteum gametophores representing five different hydration stages (hydrated (H0), dehydrated for 2 h (D2), 24 h (D24), then rehydrated for 2 h (R2) and 48 h (R48)), were sampled for transcriptome analyses. Illumina high throughput RNA-Seq technology was employed and generated more than 488.46 million reads. An in-house de novo transcriptome assembly optimization pipeline based on Trinity assembler was developed to obtain a reference Hydration-Dehydration-Rehydration (H-D-R) transcriptome comprising of 76,206 transcripts, with an N50 of 2,016 bp and average length of 1,222 bp. Comprehensive transcription factor (TF) annotation discovered 978 TFs in 62 families, among which 404 TFs within 40 families were differentially expressed upon dehydration-rehydration. Pfam term enrichment analysis revealed 172 protein families/domains were significantly associated with the H-D-R cycle and confirmed early rehydration (i.e. the R2 stage) as exhibiting the maximum stress-induced changes in gene expression.


Plant Physiology and Biochemistry | 2017

ScDREB8, a novel A-5 type of DREB gene in the desert moss Syntrichia caninervis, confers salt tolerance to Arabidopsis

Yuqing Liang; Xiaoshuang Li; Daoyuan Zhang; Bei Gao; Honglan Yang; Yucheng Wang; Kaiyun Guan; Andrew J. Wood

Salinity is a major limitation factor for crop productivity worldwide. DREB transcription factors broadly participate in plant stress response and have been extensively identified in a wide variety of plants. In this study, we characterized and analyzed the function of a novel A-5 type DREB gene ScDREB8 from the desiccation tolerant moss Syntrichia caninervis. Yeast one-hybrid experiment showed that ScDREB8 had no transactivation activity. Transient expression assay in onion epidermal cells revealed that ScDREB8 is distributed throughout the cell with no apparent specificity. Overexpression of ScDREB8 significantly increased the germination rate of Arabidopsis under salt stress and improved the salt tolerance of Arabidopsis at the seedling stage by up-regulating the expression of downstream stress-related genes and improving ROS scavenging ability. ScDREB8 is a promising candidate gene for improving crop salt stress and will provide greater insight to the molecular mechanism of stress tolerance of A-5 type DREB proteins.


Journal of Bryology | 2018

Differential fragment regeneration in Syntrichia caninervis Mitt. from the Gurbantunggut Desert of China

Lu Zhuo; Yigong Zhang; Xiaoshuang Li; Honglan Yang; Kai-Yun Guan; Andrew J. Wood; Daoyuan Zhang

Fragments of the desert moss Syntrichia caninervis Mitt. were grown on the surface of moistened sand to assess their regeneration capacity. The plant material was collected in two different years (2014 and 2015) and divided into five fragment classifications (stem apices, green leaves, yellow-green leaves, brown leaves and stems). All fragments of the stem apices, green leaves and stems regenerated within 10 days of culture while some fragments of yellow-green leaves (two 2014 fragments and one 2015 fragment) and brown leaves (three 2014 fragments and three 2015 fragments) died. Fragments of stem, stem apices and green leaves regenerated more quickly, produced longer protonemata and more shoots as compared to fragments of yellow-green and brown leaves. These differences were statistically significant but there was no difference in regeneration between the fragments from 2014 and 2015. Differential regeneration and proliferation of different plant fragments has important implications for the clonal propagation of S. caninervis in the Gurbantunggut Desert.


Journal of Arid Land | 2018

Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism

Bei Gao; Daoyuan Zhang; Xiaoshuang Li; Honglan Yang; Yuqing Liang; Mo-Xian Chen; Yuanming Zhang; Jianhua Zhang; Wood Andrew

Bryum argenteum Hedw. is a desiccation tolerant bryophyte and belongs to one of the most important components of the biological soil crusts (BSCs) found in the deserts of Central Asia. Limited information is available on rehydration-responsive proteins in desiccation tolerant plants. As a complement to our previous research analyzing the rehydration transcriptome, we present a parallel quantitative proteomic effort to study rehydration-responsive proteins. Bryophyte gametophores were desiccated (Dry) and rehydrated for 2 h (R2) and 24 h (R24). Proteins from Dry, R2 and R24 gametophores were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) to determine the relative abundance of rehydration-responsive proteins. A total of 5503 non-redundant protein sequences were identified and 4772 (86.7%) protein sequences were annotated using Gene Ontology (GO) terms and Pfam classifications. Upon rehydration 239 proteins were elevated and 461 proteins were reduced as compared to the desiccated protein sample. Differentially up-regulated proteins were classified into a number of categories including reactive oxygen species scavenging enzymes, detoxifying enzymes, Late Embryogenesis Abundant (LEA) proteins, heat shock proteins, proteasome components and proteases, and photosynthesis and translation related proteins. Furthermore, the results of the correlation between transcriptome and proteome revealed the discordant changes in the expression between protein and mRNA.


Molecular Breeding | 2014

Overexpression of Tamarix albiflonum TaMnSOD increases drought tolerance in transgenic cotton

Daoyuan Zhang; Honglan Yang; Xiaoshuang Li; Haiyan Li; Yu-Chen Wang

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Daoyuan Zhang

Chinese Academy of Sciences

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Xiaoshuang Li

Chinese Academy of Sciences

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Andrew J. Wood

Southern Illinois University Carbondale

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Bei Gao

The Chinese University of Hong Kong

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Haiyan Li

Chinese Academy of Sciences

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Yuanming Zhang

Chinese Academy of Sciences

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Yuqing Liang

Chinese Academy of Sciences

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Jiancheng Wang

Chinese Academy of Sciences

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Kaiyun Guan

Chinese Academy of Sciences

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