Hongwei Gao

A novel loop-mediated isothermal amplification method for detection of the carrot materials in foods

Carrot is an important elicitor of food allergy. In this study, we developed a novel molecular detection assay based on loop-mediated isothermal amplification technology for the detection of carrot in food materials. The assay had sensitivity up to 4xa0pg of the purified carrot DNA and had no cross-reactions with other species, such as fennel, parsley, anise, caraway, cumin, walnut, pecan, peanut, cashew, almond, pistachio, sunflower seed, sesame seed, chestnut, soy, barley, wheat, oat, rye, rice, cabbage, green pepper, onion, celery, cucumber, beef, mutton, pork, chicken and shrimp. The validation study demonstrated high reproducibility and specificity. This assay was proved to be a potential tool for the detection and label management of carrot allergens in foods.

Application of multiple displacement amplification in detection of five events of genetically modified organisms

In this study, multiple displacement amplification (MDA) was used for transgene detection, and proved to effectively improve the sensitivity of most fluorescence polymerase chain (PCR). Five events of genetically modified plants were chosen for detection in different plant species by MDA and real-time PCRs. Individual primer/probe systems based on each transgene target were used. The extracted DNA of each sample was diluted and used as a template for MDA. All extracted DNA and diluted mdaDNA were detected by real-time PCR. The results were evaluated with respect to DNA quantity, DNA purity and difference of Ct values of the isogenetic DNA extract before and after MDA (D-values). The MDA amplifying effects were largely different among genes and plants. Organelle DNA has more copies than genomic DNA by MDA. n n Key words: Multiple displacement amplification, genetically modified organisms, detection, events.