Hongxian He
DuPont
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Publication
Featured researches published by Hongxian He.
Applied and Environmental Microbiology | 2013
John E. Seip; Raymond E. Jackson; Hongxian He; Quinn Qun Zhu; Seung-Pyo Hong
ABSTRACT In the oleaginous yeast Yarrowia lipolytica, de novo lipid synthesis and accumulation are induced under conditions of nitrogen limitation (or a high carbon-to-nitrogen ratio). The regulatory pathway responsible for this induction has not been identified. Here we report that the SNF1 pathway plays a key role in the transition from the growth phase to the oleaginous phase in Y. lipolytica. Strains with a Y. lipolytica snf1 (Ylsnf1) deletion accumulated fatty acids constitutively at levels up to 2.6-fold higher than those of the wild type. When introduced into a Y. lipolytica strain engineered to produce omega-3 eicosapentaenoic acid (EPA), Ylsnf1 deletion led to a 52% increase in EPA titers (7.6% of dry cell weight) over the control. Other components of the Y. lipolytica SNF1 pathway were also identified, and their function in limiting fatty acid accumulation is suggested by gene deletion analyses. Deletion of the gene encoding YlSnf4, YlGal83, or YlSak1 significantly increased lipid accumulation in both growth and oleaginous phases compared to the wild type. Furthermore, microarray and quantitative reverse transcription-PCR (qRT-PCR) analyses of the Ylsnf1 mutant identified significantly differentially expressed genes during de novo lipid synthesis and accumulation in Y. lipolytica. Gene ontology analysis found that these genes were highly enriched with genes involved in lipid metabolism. This work presents a new role for Snf1/AMP-activated protein kinase (AMPK) pathways in lipid accumulation in this oleaginous yeast.
Archive | 2007
Howard Glenn Damude; Hongxian He; Der-Ing Liao; Quinn Qun Zhu
Applied Microbiology and Biotechnology | 2013
Zhixiong Xue; Hongxian He; Dieter Hollerbach; Daniel Joseph Macool; Narendra S. Yadav; Hongxiang Zhang; Bogdan Szostek; Quinn Qun Zhu
Lipids | 2012
Dana M. Walters Pollak; Michael W. Bostick; Hyeryoung Yoon; Jamie Wang; Dieter Hollerbach; Hongxian He; Howard Glenn Damude; Hongxiang Zhang; Narendra S. Yadav; Seung-Pyo Hong; Pamela L. Sharpe; Zhixiong Xue; Quinn Qun Zhu
Archive | 2011
Michael W. Bostick; Melissa D. Daubert; Hongxian He; Seung-Pyo Hong; Dana M. Walters Pollak; Pamela L. Sharpe; Yehong Jamie Wang; Zhixiong Xue; Narendra S. Yadav; Hongxiang Zhang; Qun Zhu
Archive | 2011
Michael W. Bostick; Hongxian He; Yougen Li; Quinn Qun Zhu
Archive | 2017
Hongxian He; Michael W. Bostick; Qun Zhu; Yougen Li
Archive | 2011
Michael W. Bostick; Hongxian He; Yougen Li; Qun Zhu
Archive | 2011
Michael W. Bostick; Melissa D. Daubert; Hongxian He; Seung-Pyo Hong; Dana M. Walters Pollak; Pamela L. Sharpe; Yehong Jamie Wang; Zhixiong Xue; Narendra S. Yadav; Hongxiang Zhang; Qun Zhu
Archive | 2011
Michael W. Bostick; Hongxian He; Yougen Li; Qun Zhu