Horacio Kido
University of California, Davis
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Featured researches published by Horacio Kido.
Analytica Chimica Acta | 2000
Horacio Kido; Angel Maquieira; Bruce D. Hammock
Microarray technology as applied to areas that include genomics, diagnostics, environmental, and drug discovery, is an interesting research topic for which different chip-based devices have been developed. As an alternative, we have explored the principle of compact disc-based microarrays. This new methodology successfully combined high-density microarrays applied via a piezoelectric inkjet applicator with circular indexing on a polycarbonate disc. As a demonstration of the principle, we ran competitive inhibition immunoassays for hydroxyatrazine, carbaryl, and molinate. The resulting microspots were approximately 75 micrometers in diameter and were visualized by using an antibody labeled with a fluorescent tag and a commercially available fluorescence scanner. The results of this work suggest that compact disc-based microarray technology can give qualitative and quantitative results and has potential for simultaneous multianalyte analyses.
Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes | 1996
Ferenc Szurdoki; Lynn L. Jaeger; Adam S. Harris; Horacio Kido; Ingrid Wengatz; Marvin H. Goodrow; A. Székács; Monika Wortberg; J. Zheng; Donald W. Stoutamire; James R. Sanborn; S. D. Gilman; A. D. Jones; Shirley J. Gee; Prabhakara V. Choudary; Bruce D. Hammock
Rapid, inexpensive, sensitive, and selective enzyme-linked immunosorbent assays (ELISAs) now are utilized in environmental science. In this laboratory, many ELISAs have been developed for pesticides and other toxic substances and also for their metabolites. Compounds for which ELISAs have recently been devised include insecticides (organophosphates, carbaryl, pyrethroids, and fenoxycarb), herbicides (s-triazines, arylureas, triclopyr, and bromacil), fungicides (myclobutanil), TCDD, and metabolites of naphthalene and toluene. New rapid assays have been developed for mercury.
Archive | 1999
Horacio Kido; Ferenc Szurdoki; Mae Sexauer Gustin; Bruce D. Hammock
Our chelate sensor method for detecting mercury is based on the high affinity of thiol-containing ligands for the mercuric ion. The method combines the simple ELISA-format with the selective complexation of mercuric ions by dithiocarbamate chelators. The first assay uses a sandwich chelate formed by a ligand immobilized on the well of an ELISA plate, mercuric ion of the analyzed sample, and another ligand bound to the reporter enzyme. Our second assay utilizes competition between the binding of mercuric ions and an organomercury-conjugate to a chelating conjugate. Specifically, it involves a chelator doped on the solid surface and a mercury-containing enzyme-tracer. Both assays were further characterized by testing them under several conditions that might be encountered during some practical applications of the assay. Finally, they were tested with river water and human saliva to yield a good correlation with spike levels.
Journal of Agricultural and Food Chemistry | 1997
Horacio Kido; Marvin H. Goodrow; Valerie N. Griffeth; Anne D. Lucas; Shirley J. Gee; Bruce D. Hammock
Bioconjugate Chemistry | 1995
Ferenc Szurdoki; Horacio Kido; Bruce D. Hammock
ACS Symposium Series | 1996
Ingrid Wengatz; Adam S. Harris; S. Douglass Gilman; Monika Wortberg; Horacio Kido; Ferenc Szurdoki; Marvin H. Goodrow; Lynn L. Jaeger; Donald W. Stoutamire; James R. Sanborn; Shirley J. Gee; Bruce D. Hammock
ACS Symposium Series | 1996
Ferenc Szurdoki; Horacio Kido; Bruce D. Hammock
Archive | 1995
Ferenc Szurdoki; Horacio Kido; Bruce D. Hammock
Archive | 1994
Bruce D. Hammock; Ferenc Szurdoki; Horacio Kido
Analytical Chemistry | 1998
Bruce D. Hammock; Horacio Kido