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Dive into the research topics where Horacio Kido is active.

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Featured researches published by Horacio Kido.


Analytica Chimica Acta | 2000

Disc-based immunoassay microarrays

Horacio Kido; Angel Maquieira; Bruce D. Hammock

Microarray technology as applied to areas that include genomics, diagnostics, environmental, and drug discovery, is an interesting research topic for which different chip-based devices have been developed. As an alternative, we have explored the principle of compact disc-based microarrays. This new methodology successfully combined high-density microarrays applied via a piezoelectric inkjet applicator with circular indexing on a polycarbonate disc. As a demonstration of the principle, we ran competitive inhibition immunoassays for hydroxyatrazine, carbaryl, and molinate. The resulting microspots were approximately 75 micrometers in diameter and were visualized by using an antibody labeled with a fluorescent tag and a commercially available fluorescence scanner. The results of this work suggest that compact disc-based microarray technology can give qualitative and quantitative results and has potential for simultaneous multianalyte analyses.


Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes | 1996

Rapid assays for environmental and biological monitoring.

Ferenc Szurdoki; Lynn L. Jaeger; Adam S. Harris; Horacio Kido; Ingrid Wengatz; Marvin H. Goodrow; A. Székács; Monika Wortberg; J. Zheng; Donald W. Stoutamire; James R. Sanborn; S. D. Gilman; A. D. Jones; Shirley J. Gee; Prabhakara V. Choudary; Bruce D. Hammock

Rapid, inexpensive, sensitive, and selective enzyme-linked immunosorbent assays (ELISAs) now are utilized in environmental science. In this laboratory, many ELISAs have been developed for pesticides and other toxic substances and also for their metabolites. Compounds for which ELISAs have recently been devised include insecticides (organophosphates, carbaryl, pyrethroids, and fenoxycarb), herbicides (s-triazines, arylureas, triclopyr, and bromacil), fungicides (myclobutanil), TCDD, and metabolites of naphthalene and toluene. New rapid assays have been developed for mercury.


Archive | 1999

Chelate Sensor Method for Mercury

Horacio Kido; Ferenc Szurdoki; Mae Sexauer Gustin; Bruce D. Hammock

Our chelate sensor method for detecting mercury is based on the high affinity of thiol-containing ligands for the mercuric ion. The method combines the simple ELISA-format with the selective complexation of mercuric ions by dithiocarbamate chelators. The first assay uses a sandwich chelate formed by a ligand immobilized on the well of an ELISA plate, mercuric ion of the analyzed sample, and another ligand bound to the reporter enzyme. Our second assay utilizes competition between the binding of mercuric ions and an organomercury-conjugate to a chelating conjugate. Specifically, it involves a chelator doped on the solid surface and a mercury-containing enzyme-tracer. Both assays were further characterized by testing them under several conditions that might be encountered during some practical applications of the assay. Finally, they were tested with river water and human saliva to yield a good correlation with spike levels.


Journal of Agricultural and Food Chemistry | 1997

Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Hydroxytriazines

Horacio Kido; Marvin H. Goodrow; Valerie N. Griffeth; Anne D. Lucas; Shirley J. Gee; Bruce D. Hammock


Bioconjugate Chemistry | 1995

Development of rapid mercury assays. Synthesis of sulfur- and mercury-containing conjugates.

Ferenc Szurdoki; Horacio Kido; Bruce D. Hammock


ACS Symposium Series | 1996

Recent Developments in Immunoassays and Related Methods for the Detection of Xenobiotics

Ingrid Wengatz; Adam S. Harris; S. Douglass Gilman; Monika Wortberg; Horacio Kido; Ferenc Szurdoki; Marvin H. Goodrow; Lynn L. Jaeger; Donald W. Stoutamire; James R. Sanborn; Shirley J. Gee; Bruce D. Hammock


ACS Symposium Series | 1996

Rapid mercury assays

Ferenc Szurdoki; Horacio Kido; Bruce D. Hammock


Archive | 1995

Development of Assay for Analysis of Hg2+Based on Sulfur-Containing Ligands

Ferenc Szurdoki; Horacio Kido; Bruce D. Hammock


Archive | 1994

Enzyme amplified competitive and sandwich chelation assays for metal ions

Bruce D. Hammock; Ferenc Szurdoki; Horacio Kido


Analytical Chemistry | 1998

Books: A delightful read

Bruce D. Hammock; Horacio Kido

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Ferenc Szurdoki

Hungarian Academy of Sciences

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Shirley J. Gee

University of California

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Ferenc Szurdoki

Hungarian Academy of Sciences

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Adam S. Harris

University of California

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Ingrid Wengatz

University of California

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Lynn L. Jaeger

University of California

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