Hsiao-Nan Hao

An efficient method for the cryopreservation of fetal human liver hematopoeitic progenitor cells.

The use of human hematopoietic progenitor cells (HPC) for transplantation requires efficient recovery methods and cryopreservation procedures. The purpose of this study was to determine cryopreservation techniques for fetal human liver (FHL) CD34+ cells. We assessed FHL HPC recovery efficiency after freezing and thawing by viability testing, fluorescence‐activated cell sorting analysis, and colony‐forming ability under different conditions. We also determined optimal cell freezing concentrations and the effect of rate‐controlled freezing on cell recovery. Lastly, cell recovery after varying freezing time periods was examined. Our results indicated that optimal cell recovery occurs when: A) cryopreservation medium consists of either 5% dimethylsulphoxide (DMSO) or 10% DMSO in combination with either 20% fetal bovine serum (FBS) or 70% FBS and when Iscoves modified Dulbeccos medium consists of not more than 10% DMSO; B) a rate‐controlled freezing device container is used; C) CD34+ cells are frozen at a concentration of 1 × 106/ml, and D) a thawing temperature of 37°C is used. These observations indicate that cryopreservation of FHL HPC is possible for up to 18 months in optimal conditions without losing hematopoietic activity.

The roles of monocytic heat shock protein 60 and Toll-like receptors in the regional inflammation response to wear debris particles

The biological response to orthopaedic wear debris is central to peri-prosthetic tissue inflammation and osteolysis, through mechanisms that include local inflammatory cytokine production. In particular, interleukin-1 beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha|) are generated in high quantities following monocyte accumulation in periprosthetic inflammatory tissue, and these cytokine combine with other inflammatory mediators to trigger osteolysis. Since the precise mechanisms involved in debris-associated inflammation remain unclear, it is important to understand how wear debris particles initially interact with inflammatory cells. We have previously demonstrated that the severity of the inflammation response is influenced by the size, shape, and quantity of particles accumulated in tissues. The current in vitro and in vivo results indicate that heat-shock protein (Hsp) expression is elevated when monocytes are exposed to wear debris particles. We have also addressed the mechanisms by which heat-shock protein 60 (Hsp60) positively modulates inflammatory cytokines via Toll-like receptor-4 (TLR4) signal transduction pathway on mononuclear cells. Furthermore, down-regulation of TLR4 expression using antisense oligonucleotides targeted to TLR4 mRNA suppressed cytokine production in both exogenous Hsp60 and particles stimulated cultures. Collectively, these data indicate that monocytic Hsp60 is an additional inducible immunoregulatory mediator in response to particle-induced cell stress.

Mechanisms Controling CD40(receptor)-Mediated Neuroimmune Pathways: The Role of Cytokines, Bacterial and Viral Antigens, and Its Cognate Ligand

Mechanisms Controling CD40(receptor)-Mediated Neuroimmune Pathways: The Role of Cytokines, Bacterial and Viral Antigens, and Its Cognate Ligand

Infection of Fetal Human Astrocytes by M-Tropic HIV Is Not Inhibited by Prostaglandin-Mediated CCR5 Downregulation

Infection of Fetal Human Astrocytes by M-Tropic HIV Is Not Inhibited by Prostaglandin-Mediated CCR5 Downregulation

FLT3/FLK2 Receptor Expression by Fetal Human Liver and Cord Blood CD34+ Cells and Ligand-mediated Colony Formation

FLT3/FLK2 Receptor Expression by Fetal Human Liver and Cord Blood CD34+ Cells and Ligand-mediated Colony Formation

Enhancement of HIV Infection of Human Fetal Liver and Cord Blood CD34+ CD4- Cells Is Mediated by Upregulation of Chemokine Receptor Expression on These Cells

Enhancement of HIV Infection of Human Fetal Liver and Cord Blood CD34+ CD4- Cells Is Mediated by Upregulation of Chemokine Receptor Expression on These Cells

Comparison of Fetal Human Liver with Cord Blood Hematopoietic Stem Cell BFU-E Colony Formation in Response to Human Erythropoietin

Comparison of Fetal Human Liver with Cord Blood Hematopoietic Stem Cell BFU-E Colony Formation in Response to Human Erythropoietin

A Human Astrocyte-Associated HIV Glycoprotein Receptor Mediates Toxic Effects of a gp120-Gelonin Conjugate

A Human Astrocyte-Associated HIV Glycoprotein Receptor Mediates Toxic Effects of a gp120-Gelonin Conjugate