Hu Huang
Johns Hopkins University
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Publication
Featured researches published by Hu Huang.
Journal of Ophthalmology | 2015
Jianbo He; Hong Wang; Ying Liu; Wen Li; Dorothy Kim; Hu Huang
Diabetic retinopathy (DR) is a leading cause of blindness in working age adults. The objective of this study is to investigate the effects of vascular endothelial growth factor receptor 1 (VEGFR1) blockade on the complications of DR. Experimental models of diabetes were induced with streptozotocin (STZ) treatment or Insulin2 gene mutation (Akita) in mice. Protein expression and localization were examined by western blots (WB) and immunofluorescence (IF). mRNA expression was quantified by PCR array and real-time PCR. The activity of VEGFR1 signaling was blocked by a neutralizing antibody called MF1. Vascular leakage was evaluated by measuring the leakage of [3H]-mannitol tracer into the retina and the IF staining of albumin. VEGFR1 blockade significantly inhibited diabetes-related vascular leakage, leukocytes-endothelial cell (EC) adhesion (or retinal leukostasis), expression of intercellular adhesion molecule- (ICAM-) 1 protein, abnormal localization and degeneration of the tight junction protein zonula occludens- (ZO-) 1, and the cell adhesion protein vascular endothelial (VE) cadherin. In addition, VEGFR1 blockade interfered with the gene expression of 10 new cytokines and chemokines: cxcl10, il10, ccl8, il1f6, cxcl15, ccl4, il13, ccl6, casp1, and ccr5. These results suggest that VEGFR1 mediates complications of DR and targeting this signaling pathway represents a potential therapeutic strategy for the prevention and treatment of DR.
Investigative Ophthalmology & Visual Science | 2012
Hu Huang; Rachel Parlier; J. Shen; Gerard A. Lutty; Vinores A. Vinores
Investigative Ophthalmology & Visual Science | 2016
Hu Huang; Wen Li; Gerard A. Lutty
Investigative Ophthalmology & Visual Science | 2015
Hu Huang
Investigative Ophthalmology & Visual Science | 2013
Hu Huang; Da'Kuawn Johnson; Dorothy Kim; Gerard A. Lutty
Investigative Ophthalmology & Visual Science | 2012
Samuel C. Yiu; Hu Huang; Guoying Sun; Gerard A. Lutty; Deepak P. Edward
Investigative Ophthalmology & Visual Science | 2011
Stanley A. Vinores; Hu Huang; Sara Van de Veire; Mansi Dalal; Richard D. Semba; Peter Carmeliet
Investigative Ophthalmology & Visual Science | 2011
Hu Huang; Stanley A. Vinores
Investigative Ophthalmology & Visual Science | 2010
Stanley A. Vinores; Hu Huang; J. Gandhi; Xiufeng Zhong; W. Xiao
Investigative Ophthalmology & Visual Science | 2010
D. O'Brien; Hu Huang; V. M. Canto-Soler