Huabin Zou

Progress in Quality Control of Herbal Medicine with IR Fingerprint Spectra

Abstract The efficiencies of herbal medicines depend on the amount of active components in them, which could vary significantly in contents. Therefore, the quality control of herbal medicines is a very important issue. This review summarizes the true and false, producing region identification and quality evaluation of herbal medicines by means of their component overlapping infrared spectra (defined as infrared fingerprint spectra or IR FPS). The true and false identification could be performed with specific peaks and their absorption ratios. The producing region and quality of herbal medicines could be determined by analyzing the IR FPS with mathematical classification methods, such as potential component analysis, cluster analysis, and correlation coefficient analysis.

Study on the co-luminescence system of Tb-Gd-BPMPHD-CTMAB and its analytical application

Abstract The study indicates that the Tb-BPMPHD-CTMAB ion association system has strong fluorescence intensity. In this system, it is found that the fluorescence can be increased by Sm3+, Eu3+, Dy3+, Tm3+ and Yb3+, as well as well as Y3+, Lu3+, Gd3+ and La3+. This is a newly found co-luminescence system. The Tb-Gd-BPMPHD-CTMAB system is studied in detail on the formation conditions; and the affecting factors of the co-luminescence effect and the system is applied to determine the trace amount of Tb3+. The experiments indicate that in the optimum determining conditions, a linear relationship is obtained between the fluorescence intensity and the Tb3+ concentration in the range 2.0 × 10−11 − 8.0 × 10−6 mol 1−1. The detection limit of Tb3+ is 2.0 × 10−12 mol 1−1. The determination of samples give satisfactory results.

Study on the co-luminescence system Sm-Gd-BPMPHD-CTMAB and its analytical application

It has been found that the Sm-BPMPHD-CTMAB ion association system shows a strong fluorescence intensity. In this system some rare earth ions, such as Lu3+, Y3+, Gd3+ and Tb3+, exert an evident fluorescence enhancement effect. The formation conditions of the system and the factors affecting the columinescence effect were studied in detail in order to determine trace amounts of Sm3+. Under optimum conditions a linear relationship was obtained between the fluorescence intensity and the Sm3+ concentration in the range of 5.0×10−8–8.0×10−6 mol·L−1. The detection limit of Sm3+ was 2.0×10−8 mol·L−1. The determination gave satisfactory results.

Study on the co-luminescence effect of Eu-Gd-BM-DPG-Triton X-100 system and its application to the determination of europium

Abstract The Eu-benzoylacetone (BM)-diphenylguanide(DPG)-Triton X-100 system, which has a strong fluorescence intensity, was studied. It was found that the fluorescence intensity can be greatly enhanced by Gd 3+ . The experiment showed that the fluorescence intensity of the system was a maximum under the following conditions: pH = 8.3 and the concentrations of Gd 3+ , BM and DPG were 5.0 × 10 −5 , 1.0 × 10 −3 and 7.5 × 10 −4 mol l −1 , respectively. At the optimum conditions, a linear relationship was obtained between fluorescence intensity and Eu 3+ concentration in the range of 2.0 × 10 −10 −2.0 × 10 −7 mol l -1 . The detection limit was 4.0 × 10 −13 mol l −1 .

Study on the fluorescence system Be-rutin-SDS-Triton X-100 and the determination of rutin

SummaryIt was found that rutin forms a fluorescent complex with Be2+, the fluorescence intensity of which can be increased thirty-one times in a mixed medium of sodium dodecyl sulfonate (SDS) and Triton X-100. A linear relationship is obtained between the fluorescence intensity and the rutin concentration in the range of 1.2×10−6 to 4.8×10−5 mol·l−1. The detection limit is 5.9×10−7 mol·l−1. Rutin traces were determined in drugs using the standard addition method. The luminescence mechanism of the system is discussed.

Study on the fluorescence system of Tb-ATP-Phen for the determination of ATP

Abstract It was found that ATP could form a ternary complex with Tb3+ and Phen, which can emit the characteristic fluorescence of Tb3+. The experiment showed that the fluorescence intensity of the system was maxmium in the following conditions: pH of the solution is 7.2, the concentrations of Tb3+ and phenanthroline (Phen) are 2.0×10-5mol.L-1 and 2.0 × 10-4mol.L-1, respectively. At the optimum conditions, a linear relationship is obtained between the fluorescence intensity and ATP concentration in the range of 1.0×10-6—1.0×10-5mol.L-1. Traces of ATP in drugs (ATP disodium salt tablets) can be determined using the standard addition method. The luminescence mechanism of the system was discussed.

Study of the fluorescence system deoxyguanylic acid-Tb (III) and the determination of deoxyguanylic acid

Abstract It was found that deoxyguanylic acid (dGMP) can form a complex with Tb(III) at pH 6.9–7.5, which then emits the strong characteristic fluorescence of Tb(III). Excitation is at 290 nm and emission wavelengths are 490 nm and 545 nm. A linear relation is obtained between the fluorescence intensity and dGMP concentration in the range 2.0 × 10 −7 to 1.0 × 10 −5 mol l −1 . The detection limit is 3.0 × 10 −8 mol l −1 . This method can determine dGMP selectively in the presence of other nucleotides (not including GMP).

Rare Earth as Fluorescence Probe to Determine 3,4-Dihydroxydroxyphenylalanine

Abstract 3,4-Dihydroxyphenylalanine can complex with Tb (III) at pH 8.0–8.5, with emission of the characteristic fluorescence of Tb(III). This method can be used for selectively determinating 3,4-dihydroxyphenylalanine in the presence of most amino acids. The experiment showed that the fluorescence intensity is a linear function of 3,4-dihydroxyphenylalanine concentration in the range of 1.1×10−8mol/1 to 1.0×10−5mol/1. The detection limit is 5×10−9mol/1. The composition ratio of Tb to 3,4-dihydroxyphenylalanine in the complex is 1:1.

Study on 1HNMR Fingerprint Spectra of Tea by Common and Variation Peak Ratio Dual-Index Sequence Analysis Method

Abstract This paper describes the application of the common and variation peak ratio dual-index sequence analysis methods to evaluate the quality of several tea samples obtained from different species and different areas based on 1HNMR fingerprint spectra (FPS). The method is able to determine the most similar sample groups and identify accurately the varieties and qualities of Blank tea, Longjing tea, and other green tea samples. Longjing tea is a famous brand of local green tea and has its own distinct characteristics. There were obvious differences between Uji tea from Japanese and Chinese green tea. The results showed that the dual index sequent analytical method provides a good approach to accurately describe the resemblance and differences among tea samples. It also can reflect teas integrity and individual difference, and is an effective method to evaluate the quality of tea samples.

Recognition of Radix Paeoiae Alba Herbs(Fam. Ranunculaceae) with HPLC Fingerprint Spectra

Abstract The relative-area difference sequence (ΔSr sequence) analytical method is proposed, with which the most similar herbal samples can be determined according to their contents. Together with common and variation peak ratio dual-index sequence analysis, one can identify the most similar sample group in the two-dimensional sequence accurately; therefore, the method can evaluate the quality of herbs.