Huaisheng Wang
Liaocheng University
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Publication
Featured researches published by Huaisheng Wang.
Biosensors and Bioelectronics | 2009
Bin Wang; Jing-Jing Zhang; Zhuang-Ying Pan; Xu-Quan Tao; Huaisheng Wang
The direct electron transfer of immobilized horseradish peroxidase (HRP) on silica-hydroxyapatite (HAp) hybrid film-modified glassy carbon electrode (GCE) and its application as H(2)O(2) biosensors were investigated. On silica/HRP-HAp/GCE, HRP displayed a fast electron transfer process accompanied with one proton participate in. This sensor exhibited an excellent electrocatalytic response to the reduction of H(2)O(2) without the aid of an electron mediator. The proposed biosensor showed good reproducibility and high sensitivity to H(2)O(2) with the detection limit of 0.35 microM. In the range of 1.0-100 microM, the catalytic reduction current of H(2)O(2) was proportional to H(2)O(2) concentration. The apparent Michaelis-Menten constant (k(m)(app)) of the biosensor was calculated to be 21.8 microM, exhibiting a high enzymatic activity and affinity for H(2)O(2).
Biosensors and Bioelectronics | 2011
Yanfen Li; Jianchun Bao; Min Han; Zhihui Dai; Huaisheng Wang
A simple method to amplify the electrochemical signal by an aptamer with 22 bases modified with CdS hollow nanospheres (CdSHNs) was described. Using the thrombin as a model, the interaction between the aptamer and CdSHNs was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and circular dichroism spectroscopy. CdSHNs promoted the electron transfer between the gold electrode and K(3)[Fe(CN)(6)] and facilitated the conformation conversion of the aptamer from hairpin to G-quadruplex after the aptamer interacted with thrombin. Under optimal conditions, the modified electrode could be used for the determination of thrombin from 0 to 33 μg mL(-1) and the sensitivity was 1.34 μA mL μg(-1)cm(-2), while the linear range of the modified electrode without the immobilization of CdSHNs was from 2.75 to 27.5 μg mL(-1) and the sensitivity was 0.062 μA mL μg(-1)cm(-2). This constructed biosensor also had a good stability, specificity, reproducibility and accuracy which could provide a promising platform for fabrication of aptamer based biosensors.
Inorganic Chemistry | 2017
Yan Huang; Xiaoyan Long; Dazhong Shen; Guizheng Zou; Bin Zhang; Huaisheng Wang
Reactive oxygen species (ROS) involved anodic charge transfer and electrochemiluminescence (ECL) of all-inorganic halide perovskite CsPbBr3 nanocrystals (NCs) were investigated in an aqueous medium with hydrogen peroxide (H2O2) as the model. CsPbBr3 NCs could be electrochemically oxidized to positively charged states by injecting holes onto the highest occupied molecular orbitals and could be chemically reduced to negatively charged states by injecting electrons onto the lowest unoccupied molecular orbitals by ROS. The charge transfer between CsPbBr3 NCs of oxidative and reductive states could bring out monochromatic ECL with onset around +0.8 V, maximum emission around 519 nm, and a full width at half-maximum around 20 nm. H2O2 could selectively enhance the anodic ECL of CsPbBr3 NCs, which not only opened a way to design a bioprocess-involved photovoltaic device with CsPbBr3 NCs but also was promising for color-selective ECL biosensing.
Biosensors and Bioelectronics | 2018
Fang Zhang; Yupeng He; Kena Fu; Li Fu; Bin Zhang; Huaisheng Wang; Guizheng Zou
A dual-wavebands-resolved electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was developed for simultaneously detecting alpha fetoprotein antigen (AFP) in greenish waveband with CdSe550 (λmax = 550 nm) nanocrystals (NCs) and carbohydrate antigen 125 (CA125) in near-infrared waveband with CdTe790 (λmax = 790 nm) NCs via one-pot ECL reaction, in which dichroic mirror works as a key part to reflect ECL from CdSe550 to one photomultiplier-tube (PMT) and transmit ECL from CdTe790 to the other PMT for dual-wavebands-resolved assay. The proposed ECL-MIA strategy was capable of simultaneously determining AFP with linearly response from 5 pg/mL to 5 ng/mL and limit of detection at 1 pg/mL, and CA125 with linearly response from 5 mU/mL to 1 U/mL and limit of detection at 1 mU/mL, with desired specificity and without obvious energy-transfer between ECL tags. The dichroic mirror assistant ECL setup is easy-to-assemble and convenient for the popularization of color-resolved multiplexing ECL assay.
Biosensors and Bioelectronics | 2006
Huaisheng Wang; Tian-Hua Li; Wen-Li Jia; Hong-Yan Xu
Electroanalysis | 2010
Yan-Li Mei; Huaisheng Wang; Yan-Fen Li; Zhuang-Ying Pan; Wen-Li Jia
Electrochimica Acta | 2011
Lianyi Xu; Jinsheng Zhao; Chuansheng Cui; Renmin Liu; Jifeng Liu; Huaisheng Wang
Biosensors and Bioelectronics | 2007
Tian-Hua Li; Wen-Li Jia; Huaisheng Wang; Ren-Min Liu
Electrochimica Acta | 2013
Liping Jia; Jifeng Liu; Huaisheng Wang
Electrochimica Acta | 2011
Bin Wang; Jinsheng Zhao; Chuansheng Cui; Renmin Liu; Jifeng Liu; Huaisheng Wang; Houting Liu