Huawei Su

Effect of prepartum maternal energy density on the growth performance, immunity, and antioxidation capability of neonatal calves

This study investigated the effect of prepartum diets differing in energy density on growth performance, immunity, and antioxidation capability of neonatal calves. Thirty Holstein dairy cows were allocated at random into 3 groups: low energy group [L; net energy of lactation (NE(L))=5.25 MJ/kg of dry matter (DM)]; medium energy group (M; NE(L)=5.88 MJ/kg of DM); and high energy group (H; NE(L)=6.48 MJ/kg of DM) at d 21 prepartum. Plasma was sampled for analysis of glucose, total protein, β-hydroxybutyrate, and nonesterified fatty acids at 21, 14, and 7 d before parturition. After calving, birth weight and measurements of the calves in each group were recorded, and blood samples were collected for analysis of CD4, CD8, CD21, IL-2, IL-4, IL-6, total antioxidant capacity, superoxide dismutase, glutathione peroxidase, and maleic dialdehyde. The results indicated that although maternal weight did not differ among L, M, and H groups at 21, 14, and 7 d before parturition, the concentrations of glucose and β-hydroxybutyrate at 14 and 7 d in the L group were decreased compared with that in the H group. In addition, nonesterified fatty acids concentrations increased significantly in the L group at 14 and 7 d before parturition compared with that in the M and H groups. Birth weight, body height, body length, abdominal circumference, thoracic girth, umbilical girth, and levels of CD4, CD4:CD8, IL-2, IL-4, total antioxidant capacity, and superoxide dismutase were decreased in calves of the L group compared with those of the H group. For the M group, CD4, CD4:CD8, and superoxide dismutase were decreased; and in the L group glutathione peroxidase and maleic dialdehyde levels were significantly increased compared with those of the H group. Reducing the maternal energy density during the last 21 d before parturition had a negative effect on growth and development, immunity, and antioxidation capability of neonatal calves.

Effects of energy density in close-up diets and postpartum supplementation of extruded full-fat soybean on lactation performance and metabolic and hormonal status of dairy cows.

This experiment was conducted to investigate the effect of energy density (ED) in the close-up period and supplementation of extruded full-fat soybean (ESB) during the first 4 wk after parturition on intake, body weight (BW), metabolic status, and performance of dairy cows. Fifty-seven Chinese Holstein cows with similar parity, previous 305-d milk yield, and expected calving date were dried off at -60 d relative to parturition and fed the standard herd dry-cow diet until -21 d relative to parturition. Energy density at low (LED, 1.25 Mcal/kg), medium (MED, 1.41 Mcal/kg) or high (HED, 1.55 Mcal/kg) levels of the close-up diets and postpartum supplementation of ESB at 0 kg/d (control, CON) or 1.5 kg/d (TRT) were used in a 3 × 2 factorial arrangement. All cows received the same CON diet from wk 5 to wk 8. As ED increased in the close-up diet, cows had higher dry matter intake and gained more BW and body condition score, and consequently were in improved energy balance status during the prepartum period, but lost more BW and more body condition score during the first 8 wk of lactation. Compared with LED cows, HED cows had higher blood concentrations of insulin and glucose, and lower nonesterified fatty acids (NEFA) prepartum, but had lower insulin concentration, higher leptin concentration and tended to have higher NEFA concentration postpartum. Milk production was not affected by the prepartum ED, although HED cows produced approximately 2 kg/d less milk than MED and LED cows during early lactation. Postpartum ESB supplementation elevated blood glucagon concentration regardless of prepartum ED during the first 4 wk of lactation. Interactive effects between prepartum ED and postpartum ESB supplementation were observed in blood concentrations of insulin, NEFA, bilirubin, total protein, albumin, and globulin. During early lactation, TRT decreased globulin concentrations in MED cows, and reduced NEFA and bilirubin concentrations in HED cows. Compared with CON, TRT cows had higher yields of milk (34.32 vs. 36.53 kg/d), milk lactose (1.63 vs. 1.74 kg/d), and solids-nonfat (2.98 vs. 3.18 kg/d), tended to have a greater yield of milk protein (1.11 vs. 1.17 kg/d), but tended to have lower milk fat percentage (4.18 vs. 3.94%) during the first 4 wk of lactation. In conclusion, compared with feeding the HED diet, feeding the LED diet during the close-up dry period had positive carryover effects on metabolism and production during early lactation, and the MED diet showed no advantage over the LED diet. Extruded full-fat soybean supplementation during the first 4 wk of lactation had positive effects on postpartum metabolic status, especially for those receiving the MED or HED diet prepartum, and resulted in an overall improved milk production during early lactation.

Effect of calcium salt of long-chain fatty acids and alfalfa supplementation on performance of Holstein bulls

The purpose of this study was to assess the effects of calcium salt of long-chain fatty acids (CSFA) and alfalfa on beef cattle in the late fattening. 48 Holstein bulls were selected and randomly divided into 4 groups, feeding four dietary that Leymus chinensis with (LC) or with no (LN) 2.4% CSFA, and alfalfa replaced 50% Leymus chinensis with (AC) or with no (AN) 2.4% CSFA. The results indicated that alfalfa improved the feed conversion rate (P < 0.05). CSFA increased serum low density lipoprotein cholesterol, and reduced the cooking loss of Longissimus muscle (P < 0.05). CSFA and alfalfa reduced Acetate/Propionate. Alfalfa and CASF had significant additive effects on the apparent digestibility of dry matter, crude protein, neutral detergent fiber, acid detergent fiber, organic matter and rumen fermentation for acetate, isobutyrate, butyrate, isovalerate, total volatile fatty acids (P < 0.05). CSFA increased microbial diversity index when compared with alfalfa (P < 0.05), but no significant differences were detected in bacterial genera abundances among diets. The relative abundances of rumen bacterial genera have significant correlation with apparent digestibility of nutrients, rumen fermentation characteristics and serum biochemical parameters (P < 0.05). These results comprehensively evaluated the additive effects of alfalfa and CSFA on the application in Holstein bulls.

Effects of Leymus chinensis replacement with whole-crop wheat hay on blood parameters, fatty acid composition, and microbiomes of Holstein bulls

This study investigated the replacement of Leymus chinensis (LC) with whole-crop wheat hay (WCWH) in the diets of Holstein bulls in the fattening stage and examined the potential effects on blood parameters, fatty acids in rumen fluid and serum, and the rumen microbiomes. In this study, 12 Holstein bulls in the fattening period (body weight = 485.0 ± 40.8 kg) were assigned to 1 of 4 dietary treatments using a 4 × 4 Latin square design. Each experimental period consisted of a 17-d adaptation period and a 5-d collection period. The dietary treatments consisted of 4 proportions of WCWH (0, 33, 67, and 100%) as a substitute for LC (designated as WCWH0, WCWH33, WCWH67, and WCWH100, respectively). On d 18 and 22 of each experiment period, blood and rumen content samples were collected for analysis, respectively. Real-time quantitative PCR was used to analyze the rumen microbiomes. The results from this study revealed no differences in the saturated, monounsaturated, and polyunsaturated long-chain fatty acid proportions of rumen liquid among the treatments. It was observed in the present trial that rumen microbiotal flora were not significantly different in the bulls fed LC compared with the bulls fed WCWH. Additionally, blood sample analysis demonstrated that the concentration of urea nitrogen in the WCWH100 group was higher than that observed in the other groups. Meanwhile, no differences were detected for other serum parameters. There were no differences in the proportions of serum saturated, monounsaturated, and polyunsaturated long-chain fatty acids among the treatments. In conclusion, our data revealed that LC can be replaced with WCWH in the diet of Holstein bulls in the fattening stage with no negative effects on the blood indicators, fatty acids, and microbiomes.

Ecological Restoration of Antibiotic-Disturbed Gastrointestinal Microbiota in Foregut and Hindgut of Cows

Antibiotically disturbed gastrointestinal microbiota needs a long period time to be restored to normal, which may cause a series of problems to the host. The understanding of restoration of the biased microbiota by antibiotics remains largely unknown. Here, we investigated the microbiota shift in foregut (rumen) and hindgut (rectum) of lactating cows after antibiotics exposure as well as after antibiotics withdrawal with (Microbiota transplantation, MT group) or without (Control, CON group) microbiota transplantation. We were able to demonstrate that microbiota in both foregut and hindgut significantly changed after 3 or 14 days of antibiotics exposure, and the changes persisted over long period of time (>18 days) after withdrawing the antibiotics. We further observed a faster restoration of microbiota in both foregut and hindgut of MT group than CON group, microbiota in foregut was mainly benefited from microbiota transplantation by restoring the alpha-diversity as well as within-group similarity, while microbiota in hindgut was primarily benefited from microbiota transplantation by reestablishing the co-occurrence network (nodes number, edges number, density, modularity as well as closeness centrality). These results together expanded our understanding of restoration of the biased microbiota by antibiotics, and may also be instructive to deal with the delayed microbiota restoration at least in cows.

Effect of parenteral administration of glutamine on autophagy of liver cell and immune responses in weaned calves

The objectives of this study were to determine the effects of an increased jugular supply of L-Gln on post-weaning growth, immune responses, intestinal morphology and autophagy of weaned calves. At 35 days of age, 24 Holstein calves (initial 50 ± 1.5 kg; 35 ± 2 day of age) were randomly allocated to four treatments, and each treatment included five male and one female calves. Holstein calves were assigned to treatments of (i) i.v. infusion of 2 l of 0.85% NaCl, Control group [C]; (ii) i.v. infusion of 8 g/day of L-Gln mixed with 2 l of 0.85% NaCl solution [L]; (iii) i.v. infusion of 16 g/day of L-Gln mixed with 2 l of 0.85% NaCl solution [M]; and (iv) i.v. infusion of 32 g/day of L-Gln mixed with 2 l of 0.85% NaCl [H]; The infusion was 2 h/day for each of 14 consecutive days starting on day 1 after weaning. Feed and water were freely available to all calves. All calves were killed on the 14th day post-weaning for measurements of autophagy of liver cell and intestinal morphology. Gln has no effect on dry matter intake (DMI) and average daily gain (ADG). Gln infusion increased quadratically the abundance of CD4+, monocyte and the ratio of CD4+/CD8+. The urea N, Gln and Glucose in plasma increased linearly with increasing Gln loads. Gln infusion increased quadratically villus height and crypt depth of intestine. The autophagy level of liver cell was improved with the Gln infusion dose increased.

Effect of increased dietary crude protein levels on production performance, nitrogen utilisation, blood metabolites and ruminal fermentation of Holstein bulls

Objective This study investigated the effect of dietary crude protein (CP) supplementation on nutrient intake, nitrogen (N) utilisation, blood metabolites, ruminal fermentation and growth performance of young Holstein bulls. Methods Twenty-one young bulls weighing 277±11.2 kg were equally divided into three groups and were offered diets formulated with low CP (LCP; 10.21% CP and 4.22% rumen degradable protein [RDP]), medium CP (MCP; 12.35% CP and 5.17% RDP) and high CP (HCP; 14.24% CP and 6.03% RDP). Yellow corn silage was used as a unique forage source and was mixed with concentrate. This mixed feed was given ad libitum to the young bulls included in the study. Results Results showed that CP intake, blood urea nitrogen, N intake, total N excretion and N balance increased linearly with an increase in dietary CP level (p<0.05). However, no significant difference was observed in nutrient digestibility among the bulls receiving the different diets. Ruminal pH (p<0.05) and ammonia nitrogen (NH3-N) concentration (p<0.01) were significantly higher in the bulls receiving the MCP and HCP diets than in those receiving the LCP diet. The bulls receiving the HCP diet showed significantly higher ruminal bacterial protein level, propionate, acetate and total volatile fatty acid (TVFA) concentrations than bulls receiving the LCP diet (p<0.05). Moreover, dietary CP level exerted a significant positive effect on the final body weight, average daily gain and gain-to-feed ratio of the bulls (p<0.05). Conclusion High dietary CP level is optimal for achieving maximum growth and high profitability without exerting a negative effect on the physiology of growing Holstein bulls.