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Dive into the research topics where Huda Ismail Abdullah is active.

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Featured researches published by Huda Ismail Abdullah.


American Journal of Physiology-renal Physiology | 2008

Calcium-sensing receptor signaling pathways in medullary thick ascending limb cells mediate COX-2-derived PGE2 production: functional significance.

Huda Ismail Abdullah; Paulina L. Pedraza; John C. McGiff; Nicholas R. Ferreri

We determined the functional implications of calcium-sensing receptor (CaR)-dependent, Gq- and Gi-coupled signaling cascades, which work in a coordinated manner to regulate activity of nuclear factor of activated T cells and tumor necrosis factor (TNF)-alpha gene transcription that cause expression of cyclooxygenase (COX)-2-derived prostaglandin E2 (PGE2) synthesis by rat medullary thick ascending limb cells (mTAL). Interruption of Gq, Gi, protein kinase C (PKC), or calcineurin (CaN) activities abolished CaR-mediated COX-2 expression and PGE2 synthesis. We tested the hypothesis that these pathways contribute to the effects of CaR activation on ion transport in mTAL cells. Ouabain-sensitive O2 consumption, an in vitro correlate of ion transport in the mTAL, was inhibited by approximately 70% in cells treated for 6 h with extracellular Ca2+ (1.2 mM), an effect prevented in mTAL cells transiently transfected with a dominant negative CaR overexpression construct (R796W), indicating that the effect was initiated by stimulation of the CaR. Pretreatment with the COX-2-selective inhibitor, NS-398 (1 microM), reversed CaR-activated decreases in ouabain-sensitive O2 consumption by approximately 60%, but did not alter basal levels of ouabain-sensitive O2 consumption. Similarly, inhibition of either Gq, Gi, PKC, or CaN, which are components of the mechanism associated with CaR-stimulated COX-2-derived PGE2 synthesis, reversed the inhibitory effects of CaR on O2 consumption without affecting basal O2 consumption. Our findings identified signaling elements required for CaR-mediated TNF production that are integral components regulating mTAL function via a mechanism involving COX-2 expression and PGE2 production.


Cell Biochemistry and Function | 2017

Effects of propolis, caffeic acid phenethyl ester, and pollen on renal injury in hypertensive rat: An experimental and theoretical approach

Ramin Ekhteiari Salmas; Mehmet Fuat Gulhan; Serdar Durdagi; Engin Sahna; Huda Ismail Abdullah; Zeliha Selamoglu

The objective of this study was to evaluate the antioxidant effects of propolis, caffeic acid phenethyl ester (CAPE; active compound in propolis), and pollen on biochemical oxidative stress biomarkers in rat kidney tissue inhibited by Nω‐nitro‐L‐arginine methyl ester (L‐NAME). The biomarkers evaluated were paraoxonase (PON1), oxidative stress index (OSI), total antioxidant status (TAS), total oxidant status (TOS), asymmetric dimethylarginine (ADMA), and nuclear factor kappa B (NF‐κB). TAS levels and PON1 activity were significantly decreased in kidney tissue samples in the L‐NAME‐treated group (P < 0.05). The levels of TAS and PONI were higher in the L‐NAME plus propolis, CAPE, and pollen groups compared with the L‐NAME‐treated group. TOS, ADMA, and NF‐κB levels were significantly increased in the kidney tissue samples of the L‐NAME‐treated group (P < 0.05). However, these parameters were significantly lower in the L‐NAME plus propolis, CAPE, and pollen groups (P < 0.05) compared with rats administered L‐NAME alone (P < 0.05). Furthermore, the binding energy of CAPE within catalytic domain of glutathione reductase (GR) enzyme as well as its inhibitory mechanism was determined using molecular modeling approaches. In conclusion, experimental and theoretical data suggested that oxidative alterations occurring in the kidney tissue of chronic hypertensive rats may be prevented via active compound of propolis, CAPE administration.


Journal of Biomolecular Structure & Dynamics | 2018

The effects of pollen, propolis, and caffeic acid phenethyl ester on tyrosine hydroxylase activity and total RNA levels in hypertensive rats caused by nitric oxide synthase inhibition: experimental, docking and molecular dynamic studies

Ramin Ekhteiari Salmas; Serdar Durdagi; Mehmet Fuat Gulhan; Merve Duruyurek; Huda Ismail Abdullah; Zeliha Selamoglu

The objective of the present study was to evaluate the effects of propolis, pollen, and caffeic acid phenethyl ester (CAPE) on tyrosine hydroxylase (TH) activity and total RNA levels of Nω-nitro-L-arginine methyl ester (L-NAME) inhibition of nitric oxide synthase in the heart, adrenal medulla, and hypothalamus of hypertensive male Sprague dawley rats. The TH activity in the adrenal medulla, heart, and hypothalamus of the rats was significantly increased in the L-NAME group vs. control (p < 0.05). Treatment with L-NAME led to a significant increase in blood pressure (BP) in the L-NAME group compared to control (p < 0.05). These data suggest that propolis, pollen, and CAPE may mediate diminished TH activity in the heart, adrenal medulla, and hypothalamus in hypertensive rats. The decreased TH activity may be due to the modulation and synthesis of catecholamines and BP effects. In addition, the binding mechanism of CAPE within the catalytic domain of TH was investigated by means of molecular modeling approaches. These data suggest that the amino acid residues, Glu429 and Ser354 of TH may play a pivotal role in the stabilization of CAPE within the active site as evaluated by molecular dynamics (MD) simulations. Gibbs binding free energy (ΔGbinding) of CAPE in complex with TH was also determined by post-processing MD analysis approaches (i.e. Poisson-Boltzmann Surface Area (MM-PBSA) method).


American Journal of Physiology-renal Physiology | 2002

Calcium-sensing receptor-mediated TNF production in medullary thick ascending limb cells.

Dairong Wang; Paulina L. Pedraza; Huda Ismail Abdullah; John C. McGiff; Nicholas R. Ferreri


American Journal of Physiology-renal Physiology | 2006

NFAT regulates calcium-sensing receptor-mediated TNF production

Huda Ismail Abdullah; Paulina L. Pedraza; Shoujin Hao; Karin D. Rodland; John C. McGiff; Nicholas R. Ferreri


American Journal of Physiology-renal Physiology | 2008

CaR activation increases TNF production by mTAL cells via a Gi-dependent mechanism

Huda Ismail Abdullah; Paulina L. Pedraza; John C. McGiff; Nicholas R. Ferreri


Fertility and Sterility | 2007

Effects of the components of hormone therapy on matrix metalloproteinases in breast-cancer cells: an in vitro study

Maher Ali Abdallah; Huda Ismail Abdullah; Steven Kang; Douglas D. Taylor; Steven T. Nakajima; Cicek Gercel-Taylor


Archive | 2010

4 Angiotensin II Regulation of AT1 and D3 Dopamine Receptors in Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats

Nicholas R. Ferreri; Dairong Wang; Paulina L. Pedraza; Huda Ismail Abdullah; John C. McGiff


The FASEB Journal | 2009

Differential effects of TNF receptor 1 and 2 deletion on blood pressure and Ang II type I receptor (AT1R) expression

Chun Cheng Andy Chen; Huda Ismail Abdullah; Paulina L. Pedraza; Shoujin Hao; John C. McGiff; Nicholas R. Ferreri


The FASEB Journal | 2007

Calcium sensing receptor (CaR)-mediated TNF production in the medullary thick ascending limb (mTAL) is Gi-coupled

Huda Ismail Abdullah; John C. McGiff; Nicholas R. Ferreri

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John C. McGiff

New York Medical College

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Shoujin Hao

New York Medical College

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Dairong Wang

New York Medical College

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