Huihong Zhao

The complete mitochondrial genome of the Culter recurviceps (Teleostei, Cyprinidae)

Abstract We present the complete mitochondrial genome of the Culter recurviceps in this study. The mitochondrial genome is 16,622 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene order and composition of C. recurviceps mitochondrial genome was similar to that of most other vertebrates. The nucleotide compositions of the light strand are 31.36% of A, 24.77% of T, 27.77% of C and 16.09% of G. With the exception of ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. Two copies of tandem repeat sequence (24 bp) was observed in the end of the 123 bp in control region.

The complete mitochondrial genome of the Hemibagrus guttatus (Teleostei, Bagridae)

Abstract The complete mitochondrial genome of the Hemibagrus guttatus was presented in this study. The mitochondrial genome is 16,523 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene order and composition of H. guttatus mitochondrial genome was similar to that of most other vertebrates. The nucleotide compositions of the light strand are 31.73% of A, 25.42% of C, 28.02% of T and 14.83% of G. With the exception of the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand.

The complete mitochondrial genome of the Hemibarbus medius (Cypriniformes, Cyprinidae)

Abstract We present the complete mitochondrial genome of the Hemibarbus medius in this study. The mitochondrial genome is 16,611 bp in length and consists of 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a control region. The nucleotide compositions of the light strand are 29.82% of A, 27.22% of C, 25.93% of T, and 17.04% of G. With the exception of ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. All the protein-coding genes begin with an ATG initiation codon except for COXІ with GTG. Four types of termination codons revealed are TAA (ND1, COXІ, ATP8, ND4L, ND5), T (ND2, ND3, COXП, COXШ, CYTB), TA (ND4, ATP6), and TAG (ND6). Most genes are either abutted or overlapped and many features keep consistent with the other vertebrates.

The complete mitochondrial genome of the Hemibagrus wyckioides (Siluriformes, Bagridae)

Abstract We present the complete mitochondrial genome of the Hemibagrus wyckioides in this study. The mitochondrial genome is 16,525 bp in length and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. Its mitochondrial gene order and composition are similar to that of most other vertebrates. The nucleotide compositions of the light strand are 31.47% of A, 26.72 % of C, 26.62% of T, and 15.19% of G. With the exception of ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand.

The complete mitochondrial genome of the Clarias fuscus (Siluriformes, Clariidae)

Abstract We present the complete mitochondrial genome of the Clarias fuscus in this study. The mitochondrial genome is 16,525 bp in length and consists of 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a control region. The nucleotide compositions of the light strand are 31.17% of A, 27.54% of C, 25.34% of T, and 14.95% of G. With the exception of ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. All the protein-coding genes begin with an ATG initiation codon except for COXІ with GTG. Five types of termination codons revealed are TAA (COXІ, ATP8, ND4L), T (ND2, ND3, ND4, COXП, Cytb), AGG (COXШ), TA (ATP6), and TAG (ND1, ND5, ND6). Most genes are either abutted or overlapped and many features keep consistent with the other vertebrates.

The complete mitochondrial genome of the Anabas testudineus (Perciformes, Anabantidae)

Abstract We present the complete mitochondrial genome of the Anabas testudineus in this study. The mitochondrial genome is 16,603 bp in length and consists of 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a control region. The nucleotide compositions of the light strand are 29.40% of C, 28.63% of A, 25.79% of T, and 16.18% of G. With the exception of ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. All the protein-coding genes begin with an ATG initiation codon except for COXІ with GTG. Four types of termination codons revealed are TAA (COXІ, ATP8, ND4L, CYTB), T (COXП, ND3, ND4), TA (ND2, ATP6, COXШ), and TAG (ND1, ND5, ND6). Most genes are either abutted or overlapped and many features keep consistent with the other vertebrates.

The complete mitochondrial genome of the amoy croaker Argyrosomus amoyensis (Perciformes, Sciaenidae).

Abstract We present the complete mitochondrial genome of Argyrosomus amoyensis in this study. The mitochondrial genome is 16,490 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. All of them are encoded on the heavy strand except ND6 and 8 tRNA genes on the light strand. The nucleotide compositions of the light strand are 31.02% of G, 26.96% of T, 25.31% of A and 16.71% of C. All the protein-coding genes begin with ATG initiation codon, and 5 types of inferred termination codons are AGA (COXI), T (COXII, ND3, ND4, Cytb), TA (ND2, COXIII), TAA (ATP8, ATP6, ND4L, ND5, ND6) and TAG (ND1). There are 11 intergenic spacers, 7 gene overlaps and no tandem repeat sequence. Gene arrangement and distribution are consistent with the typical vertebrates.

Molecular cloning of the insulin-like growth factor 3 and difference in the expression of igf genes in orange-spotted grouper (Epinephelus coioides).

Insulin-like growth factor (Igf) is the key regulator for development, growth, and reproduction. In most vertebrate species, the Igf family has two forms: Igf1 and Igf2. A novel form of Igf, termed Igf3, was recently discovered in fish. In the present study, we isolated igf3 from the orange-spotted grouper (Epinephelus coioides). The orange-spotted grouper igf3 consisted of a full-length cDNA of 1014 nucleotides with an open reading frame (ORF) of 597 bp, encoding for proteins of 199 amino acid residues in length. Tissue distribution analysis showed that igf1 widely expressed with the highest expression in the pituitary and liver. igf2 was expressed highly in all the tissues except the olfactory bulb, while igf3 showed the highest expression in the ovary, and moderate expression in brain areas. The expression profiles of three igf genes during the ovarian development and growth hormone (Gh) and human chorionic gonadotropin (hCG) treatment were also investigated. Three igf genes exhibited different expression patterns during the ovarian development, and showed different responses to the Gh and hCG treatments, appearing to play distinct roles in ovarian development. The present study provides further evidence for the existence of an intraovarian Igf system in orange-spotted grouper.

The complete mitochondrial genome of the Plectorhinchus cinctus (Teleostei, Haemulidae)

Abstract We present the complete mitochondrial genome of the Plectorhinchus cinctus in this study. The mitochondrial genome is 16,523 bp in length and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The nucleotide compositions of the light strand are 31.30% of C, 27.66% of A, 24.54% of T, and 16.50% of G. With the exception of ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. All the protein-coding genes begin with an ATG initiation codon except for COX1 with GTG. Five types of termination codons revealed are TAA (ND1, ND2, ATP8, ND4L, ND5), T (COXП, ND3, ND4, CYTB), TA (ATP6, COXШ), AGG (COXІ) and TAG (ND6).

Characterization of the Complete Mitochondrial Genome Sequences of Three Croakers (Perciformes, Sciaenidae) and Novel Insights into the Phylogenetics

The three croakers (Nibea coibor, Protonibea diacanthus and Argyrosomus amoyensis, Perciformes, Sciaenidae) are important commercial species inhabiting the Eastern Indian Ocean and Western Pacific. Molecular data employed in previous research on phylogenetic reconstruction have not been adequate and complete, and systematic and comprehensive phylogenetic relationships for these fish are unresolved. We sequenced the complete mitochondrial genomes of the three croakers using next-generation sequencing for the first time. We analyzed the composition and phylogenies between 19 species in the family Sciaenidae using the mitochondrial protein coding sequences of 204 species in the Series Eupercaria. We present the characterization of the complete mitochondrial genome sequences of the three croakers. Gene arrangement and distribution of the three croakers are canonically identical and consistent with other vertebrates. We found that the family Sciaenidae is an independent branch that is isolated from the order Perciformes and does not belong to any extant classification. Therefore, this family is expected to belong to a new classification at the order level and needs further analysis. The evolution of Sciaenidae has lagged far behind the Perciformes differentiation. This study presents a novel insight into the phylogenetics of the family Sciaenidae from the order Perciformes and facilitates additional studies on the evolution and phylogeny of Series Eupercaria.