Hüseyin Türker

Effects of ultraviolet radiation on the stratum corneum of skin in mole rats

Abstract It is likely that the UV rays will affect the epidermis of mammals. All these negative effects justify the studies on the relation between the UV rays and epidermis. The purpose of this study was to investigate the ultrastructural effects of ultraviolet C radiation (UVC) on the stratum corneum of mole rats epidermis. Mole rats were divided into two as the control and experiment groups. The control group did not receive any radiation while the other groups were irradiated with UV radiation for 52, 112 and 168h. The skin samples were prepared and analyzed by transmission electron microscopy. After the examination, stratum basale, stratum spinosum, stratum granulosum and stratum corneum layers were distinguished. The most drastic effects of UVC were noted in stratum corneum. Lacunae formations and unkeratinized cytoplasmic residues were observed within the horny cells. Depending on dosage and exposal period of the UVC radiation, ultrastructural changes occurred in the stratum corneum on mole rats epidermis.

Ultrastructural effects of ultraviolet C radiation on the stratum basale of mole rats epidermis

Abstract The ultrastructure of the epidermis of mole rats (Spalax leucodon) was studied after irradiation with ultraviolet (UV) light (λmax=254nm; 0.00147Jcm−2s−1; for periods of 52, 112 and 168h) by using transmission electron microscope (TEM). After irradiation, Vacuolation in cytoplasm and mitochondria, and wrinkled nucleus were found in the stratum basal cells. Also, pathological aggregations of tonofilaments are formed in the desmosomes in these cells. These findings clearly demonstrated the harmful effects of ultraviolet C radiation on the stratum basale. The degree of pathological changes occurred depending on exposure time and radiation dosage applied.

The effects of ultraviolet C radiation on the ultrastructure of the liver cells of mole rats.

ABSTRACT The aim of this study was to elucidate the ultrastructural changes in the liver cells of mole rats (Spalax leucodon) exposed to ultraviolet radiation (UVR). Thirteen mole rats used in this study were caught from nature. They were divided into four groups. The first group was separated as a control and was not given any radiation. The rest were exposed to ultraviolet C (UVC) radiation for 7, 14, and 21 days. The electron microscopic examinations revealed that significant ultrastructural changes occurred in the liver tissue. These changes were the reduction in cytoplasmic organelles, dilatation in rough endoplasmic reticulum, impairment of nucleus membrane, and broadened and vacuolated mitochondria in the cytoplasm. Also, UVC radiation caused significant changes in liver enzymes of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and gama-glutamiltransferase values. After long-term exposure to radiation, some excessive ultrastructural changes occurred. These results indicated that longer exposure to UVR would cause more ultrastructural effects on the liver cells and liver enzymes.

The effect of ultraviolet radiation of pancreatic exocrine cells in mole rats: An ultrastructural study

Abstract The purpose of this study was to investigate the ultrastructural effects of ultraviolet C (UVC) radiation on the pancreatic exocrine cells of mole rats. The mole rats were divided into two groups as control and test groups. Control group did not receive any radiation. The other group was irradiated with UV radiation for 14 and 28 days. The pancreatic tissue samples were prepared and then analyzed through transmission electron microscope. Depending on the radiation exposure, it is likely to say that the zymogen granules decreased to more than 70 per cent for the control group and the dilation of rough endoplasmic reticulum and vacuolization of mitochondria increased in the pancreatic exocrine cells at the 14 days of radiation as compared with the control group; however, they were easily observed in the 28 days of radiation exposure. Particularly in the 28 days of radiation, the zymogen granules decreased and vacuolated, and the rough endoplasmic reticulum was frequently shortened and dilated. These findings clearly demonstrated the effects of UVC radiation on pancreatic exocrine cells in an exposure-period dependent manner.

Effects of ultraviolet radiation on mole rats kidney: A histopathologic and ultrastructural study

Abstract The purpose of this study was to realize the ultrastructural effects of ultraviolet radiation on the kidney tissue cells of mole rats (Spalax leucodon). The mole rats of 180–200 g body weight were divided into the control and radiation-trial groups. The control group was not given any radiation. The other groups were irradiated with artificially produced UVC radiation for 14, 28 and 60 days. The kidney tissue samples were prepared at the end of experiments and analyzed by the light and electron microscope. Several effects were observed in the kidney tissues cells analyzed in accordance with the dose magnitude of radiation. These results clearly show the detrimental effects of UVC radiation on kidney tissue cells in exposure periods dependent on radiation dose and exposure time.

Histological and ultrastructural analyses of mole rats lung cells exposed to ultraviolet radiation

Abstract The purpose of this study was to investigate the ultrastructural effects of ultraviolet C radiation on the lung cells of mole rats. Mole rats were divided into controlled and experiment groups. Controlled group did not receive any radiation. The other groups were irradiated with UV radiation for 14 and 28 days. The lung tissue samples were prepared and analyzed by light and transmission electron microscopy. A number of effects were observed in some groups which suggest some magnitude of UVC radiation affected in the lung tissue. The first changes detected in mole rats lung tissue after 14 days UVC radiation consisted of deformation in alveolar cells and in the interstitial space. There became a deformation in the alveolar epithelial cells, endothelial cells and interstitium space of the lung cells. After 28 days of UVC exposure, there happened a complete destruction of a large fraction in the capillaries. These cells showed signs of necrosis, irregularity, and their cytoplasm contained vacuoles with containing fluid-filled substantives. There happened an increase in capillary volume and a destruction in the collagen fibrins. Some destruction occurred concurrently in the alveolar epithelial cells. These findings clearly demonstrated the ultrastructural effects of UVC radiation on lung cells in an exposure-period dependent manner.