Husnain Ahmed

On-Demand Droplet Capture and Release Using Microwell-Assisted Surface Acoustic Waves

We demonstrate an acoustofluidic platform that uses surface acoustic waves (SAWs) for the facile capture of droplets inside microwells and their on-demand release. When the ac signal applied to the device is tuned to modulate the location of the SAW, the SAW-based acoustic radiation force retracts or pushes the droplets into or out of one of three microwells fabricated inside a microchannel to selectively capture or release the droplet.

Acoustic impedance-based manipulation of elastic microspheres using travelling surface acoustic waves

We present a method for size-independent manipulation of elastic polystyrene (PS), poly(methyl methacrylate) (PMMA), and fused silica (FS) microspheres that uses travelling surface acoustic waves (TSAWs). Normally incident TSAWs originating from an interdigitated transducer (IDT) were used to separate similar-sized pairs of PS and PMMA or PS and FS elastic particles by producing distinct lateral deflections across laminar streamlines in a continuous flow microfluidic channel. Elastic particles with similar diameters but different acoustic impedances exhibit significantly different deflection characteristics when exposed to TSAW-based acoustic radiation forces (ARFs). For instance, exposing a mixture of PS and PMMA particles with similar diameters (∼5 μm) to TSAWs with frequencies of 140 MHz and 185 MHz produces larger deflections of the PS and PMMA particles, respectively. This difference arises because of the resonance of the elastic particles with the incoming acoustic waves at certain frequencies. Similar particle deflection characteristics were observed for mixtures of PS and FS particles with comparable diameters (3/3, 4.8/5, 10/10 μm). This difference in deflection distances was used to experimentally characterize the non-linear behavior of the ARFs acting on particles (3–10 μm) exposed to a range of TSAW frequencies (120–205 MHz). Our experimental results can be explained by plotting the acoustic radiation force factor (FF) against the TSAW frequency (fTSAW) and the dimensionless Helmholtz number (1 < κ < 4), which is calculated by using a theoretical model of an elastic microsphere suspended in a fluid.

Vertical Hydrodynamic Focusing and Continuous Acoustofluidic Separation of Particles via Upward Migration

Abstract A particle suspended in a fluid within a microfluidic channel experiences a direct acoustic radiation force (ARF) when traveling surface acoustic waves (TSAWs) couple with the fluid at the Rayleigh angle, thus producing two components of the ARF. Most SAW‐based microfluidic devices rely on the horizontal component of the ARF to migrate prefocused particles laterally across a microchannel width. Although the magnitude of the vertical component of the ARF is more than twice the magnitude of the horizontal component, it is long ignored due to polydimethylsiloxane (PDMS) microchannel fabrication limitations and difficulties in particle focusing along the vertical direction. In the present work, a single‐layered PDMS microfluidic chip is devised for hydrodynamically focusing particles in the vertical plane while explicitly taking advantage of the horizontal ARF component to slow down the selected particles and the stronger vertical ARF component to push the particles in the upward direction to realize continuous particle separation. The proposed particle separation device offers high‐throughput operation with purity >97% and recovery rate >99%. It is simple in its fabrication and versatile due to the single‐layered microchannel design, combined with vertical hydrodynamic focusing and the use of both the horizontal and vertical components of the ARF.

In-droplet microparticle separation using travelling surface acoustic wave

Droplets in microfluidic systems can contain microscale objects such as cells and microparticles. The control of the positions of microscale objects within a microchannel is crucial for practical applications in not only continuous-flow-based but also droplet-based systems. This paper proposes an active method for the separation of microparticles inside moving droplets which uses travelling surface acoustic waves (TSAWs). We demonstrate the preconcentration and separation of 5 and 10 μm polystyrene microparticles in moving water-in-oil droplets through the application of TSAWs with two different frequencies. The microparticles inside the droplets are affected by the acoustic radiation force induced by the TSAWs to move laterally in the direction of the TSAW propagation and are thereby separated according to their size. In-droplet separation is then demonstrated through droplet splitting at a Y-junction. Compared to our previous studies, this acoustic approach offers the label-free and on-demand separation of different-sized micro-objects in moving droplets. The present method has potential uses such as in-droplet sample purification and enrichment.

A Pumpless Acoustofluidic Platform for Size-Selective Concentration and Separation of Microparticles

We have designed a pumpless acoustofluidic device for the concentration and separation of different sized particles inside a single-layered straight polydimethylsiloxane (PDMS) microfluidic channel. The proposed device comprises two parallel interdigitated transducers (IDTs) positioned underneath the PDMS microchannel. The IDTs produce high-frequency surface acoustic waves that generate semipermeable virtual acoustic radiation force field walls that selectively trap and concentrate larger particles at different locations inside the microchannel and allow the smaller particles to pass through the acoustic filter. The performance of the acoustofluidic device was first characterized by injecting into the microchannel a uniform flow of suspended 9.9 μm diameter particles with various initial concentrations (as low as 10 particles/mL) using a syringe pump. The particles were trapped with ∼100% efficiency by a single IDT actuated at 73 MHz. The acoustofluidic platform was used to demonstrate the pumpless separation of 12.0, 4.8, and 2.1 μm microparticles by trapping the 12 and 4.8 μm particles using the two IDTs actuated at 73 and 140 MHz, respectively. However, most of the 2.1 μm particles flowed over the IDTs unaffected. The acoustofluidic device was capable of rapidly processing a large volume of sample fluid pumped through the microchannel using an external syringe pump. A small volume of the sample fluid was processed through the device using a capillary flow and a hydrodynamic pressure difference that did not require an external pumping device.

Acoustic Wave-Driven Functionalized Particles for Aptamer-Based Target Biomolecule Separation

We developed a hybrid microfluidic device that utilized acoustic waves to drive functionalized microparticles inside a continuous flow microchannel and to separate particle-conjugated target proteins from a complex fluid. The acoustofluidic device is composed of an interdigitated transducer that produces high-frequency surface acoustic waves (SAW) and a polydimethylsiloxane (PDMS) microfluidic channel. The SAW interacted with the sample fluid inside the microchannel and deflected particles from their original streamlines to achieve separation. Streptavidin-functionalized polystyrene (PS) microparticles were used to capture aptamer (single-stranded DNA) labeled at one end with a biotin molecule. The free end of the customized aptamer15 (apt15), which was attached to the microparticles via streptavidin-biotin linkage to form the PS-apt15 conjugate, was used to capture the model target protein, thrombin (th), by binding at exosite I to form the PS-apt15-th complex. We demonstrated that the PS-apt15 conjugate selectively captured thrombin molecules in a complex fluid. After the PS-apt15-th complex was formed, the sample fluid was pumped through a PDMS microchannel along with two buffer sheath flows that hydrodynamically focused the sample flow prior to SAW exposure for PS-apt15-th separation from the non-target proteins. We successfully separated thrombin from mCardinal2 and human serum using the proposed acoustofluidic device.

Microfluidic flow switching via localized acoustic streaming controlled by surface acoustic waves

We propose an acoustic flow switching device that utilizes high-frequency surface acoustic waves (SAWs) produced by a slanted-finger interdigitated transducer. As the acoustic field induced by the SAWs was attenuated in the fluid, it produced an acoustic streaming flow in the form of a pair of symmetrical microvortices, which induced flow switching between two fluid streams in a controlled manner. The microfluidic device was composed of a piezoelectric substrate attached to a polydimethylsiloxane (PDMS) microchannel having an H-shaped junction that connected two fluid streams in the middle. The two immiscible fluids, separated by the PDMS wall, flowed in parallel, briefly came in contact at the junction, and separated again into the downstream microchannels. The acoustic streaming flow induced by the SAWs rotated the fluid streams within the microchannel cross-section, thereby altering the respective positions of the two fluids and directing them into the opposite flow paths. The characteristics of the flow switching mechanism were investigated by tuning the input voltage and the flowrates. On-demand acoustic flow switching was successfully achieved without additional moving parts inside the microchannel. This technique may be useful for fundamental studies that integrate complex experimental platforms into a single chip.

Characterization of microchannel anechoic corners formed by surface acoustic waves

Surface acoustic waves (SAWs) generated in a piezoelectric substrate couple with a liquid according to Snells law such that a compressional acoustic wave propagates obliquely at a Rayleigh angle ( θ t) inside the microchannel to form a region devoid of a direct acoustic field, which is termed a microchannel anechoic corner (MAC). In the present study, we used microchannels with various heights and widths to characterize the width of the MAC region formed by a single travelling SAW. The attenuation of high-frequency SAWs produced a strong acoustic streaming flow that moved the particles in and out of the MAC region, whereas reflections of the acoustic waves within the microchannel resulted in standing acoustic waves that trapped particles at acoustic pressure nodes located within or outside of the MAC region. A range of actuation frequencies and particle diameters were used to investigate the effects of the acoustic streaming flow and the direct acoustic radiation forces by the travelling as well as stand...

Sheathless Focusing and Separation of Microparticles Using Tilted-Angle Traveling Surface Acoustic Waves

Sheathless focusing and separation of microparticles is an important preprocessing step in various biochemical assays in which enriched sample isolation is critical. Most of the previous microfluidic particle separation techniques have used sheath flows to achieve efficient sample focusing. The sheath flow dilutes the analyte and requires additional microchannels and accurate flow control. We demonstrated a tilted-angle traveling surface acoustic wave (taTSAW)-based sheathless focusing and separation of particles in a continuous flow. The proposed device consists of a piezoelectric substrate with a pair of interdigitated transducers (IDTs) deposited at two different angles relative to the flow direction. A Y-shaped polydimethylsiloxane (PDMS) microchannel having one inlet and two outlet ports was positioned on top of the IDTs such that the acoustic energy coupling into the fluid was maximized and wave attenuation by the PDMS walls was minimized. The two IDTs independently produced high-frequency taTSAWs, which propagated at ±30° with respect to the flow direction and imparted a direct acoustic radiation force onto the target particles. A sample mixture of 4.8 and 3.2 μm particles was focused and then separated by the actuation of the IDTs at 194 and 136 MHz frequencies, respectively, without using an additional sheath flow. The proposed taTSAW-based particle separation device offered a high purity >99% at the both outlets over a wide range of flow speeds (up to 83.3 mm/s).