Hüsnü Aydın

The Effects of Local and Systemic Administration of Proline on Wound Healing in Rats

ABSTRACT Purpose: Wound healing consists of a sequence of complex molecular and cellular events. Collagen is composed mainly of proline and hydroxyproline. Proline and hydroxyproline constitute 1/3 of the amino acids in collagen, which makes up approximately 30% of the proteins within the body. The hydroxylation of proline found in collagen determines the stability of the triple helical structure of collagen. In this study, we examined the effects of local and systemic administration of proline on wound healing. Materials and Methods: 24 female Sprague-Dawley rats were used in the study and divided into three groups. Group 1: The defect created in the backs of the subjects was left to secondary healing. Group 2: 200 µl proline per day was administered topically for 30 days on the defect in the backs of the subjects. Group 3: 200 µl per day was administered intraperitoneally for 30 days on the defect in the backs of the subjects. Results: On day 21, there was a statistically significant difference between the groups in terms of the mean re-epithelialization score. On days 7 and 14, there was a statistically significant difference between the groups in terms of the mean granulation score. On days 7, 14, and 21, there was a statistically significant difference between the groups in terms of the mean collagen accumulation score. On day 30, there was a statistically significant difference between Groups 1 and 3 in terms of the mean E-mode score on mechanical tensile test. Conclusion: Our study confirmed that proline has positive effects on wound healing. However, it revealed that systemic administration of proline is more effective than local administration of proline.

The effect of demographic, biochemical and patholojical parameters on survival in colorectal cancer

Aim: Colorectal cancer is one of the most commonly encountered malign tumors. In this study we studied the patients who have been operated for colorectal cancer retrospectively, to determine the parameters if they are prognostic or not. Materials and Methods: 115 patients who had been operated on because of CRC were evaluated. Gender, age, place of tumor, local invasion, cancer history in first-degree relatives, chemotherapy history, histological grade, T stage, tumor size, total number of resected lymph nodes, metastatic lymph node number, positive lymph node ratio (PLNR),CEA, CA 19-9, albumin, CRP, survival, stage of disease were recorded and those parameters were compared with each other in two groups according to survival during follow-up. Results: There were not any statistically difference as regard to age, young patients, gender, tumor location and family history between two groups ( p=0,411 p=0,545 p=0,656 p=0.177 p=0,659). However, statistically significant parameters in patients who died during follow-up were histological grade, T stage, the number of metastatic lymph node, stage, PLNR, CEA, CA 19-9 and CRP (p=0.034 for CA 19-9 and p